Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
28 Cards in this Set
- Front
- Back
|
What are the different domains and kingdoms? |
Back (Definition) |
|
|
What is a hypha? |
In fungi, it’s an individual strand or filament that consists of many cells. |
|
|
What type of fungi is unicellular? |
Yeasts |
|
|
Which kingdom are heterotrophic decomposers? |
Fungi |
|
|
Are any fungi photosynthetic? |
Very few |
|
|
What is a distinguishing characteristic of domain archaea? |
Extremophilic |
|
|
Which type of Protista has chloroplasts and carry on photosynthesis? |
Algae |
|
|
What are the four phylums? How are they characterized? |
Phylum Chytridiomycota - they basically lack motility, but the gametes of the chytrids have flagella. Phylum Zygomycota - sporangiosphores Phylum Ascomycota - ascospores and condiospores Phylum Basidiomycota - basidia and basidiospores |
|
|
What are the three requirements to grow bacteria in the lab? |
1. A culture medium that provides all the essential requirements for growth 2. Techniques for preventing contamination 3. Techniques for isolating the different species of bacteria |
|
|
Why is agar the ideal solidifying agent? |
1. It is readily rehydrated 2. It is easily sterilized by heating 3. It is not digested by most species of bacteria 4. It can be incubated at a wide range of temperatures |
|
|
Why is agar the ideal solidifying agent? |
1. It is readily rehydrated 2. It is easily sterilized by heating 3. It is not digested by most species of bacteria 4. It can be incubated at a wide range of temperatures |
|
|
What are the main rules in aseptic technique? |
All materials and equipment involved must be sterile After sterilization, avoid contaminating the media with the outside environment. |
|
|
What are the possible results in a blood agar? |
Alpha hemolysis (incomplete) = agar around colonies turns green Beta hemolysis (complete) = agar turns colorless Gamma hemolytic (non hemolytic) = no changes to agar |
|
|
Why would you use a mconkeys agar? |
Selective for gram negative organisms because it inhibits the growth of gram positive. |
|
|
Why would you use a mconkeys agar? |
Selective for gram negative organisms because it inhibits the growth of gram positive. |
|
|
What are the possible results in mconkeys agar? |
Lactose fermenting colonies can turn pink and non lactose fermenting colonies remain colorless but the agar plate turns yellow. |
|
|
Why would you use a mconkeys agar? |
Selective for gram negative organisms because it inhibits the growth of gram positive. |
|
|
What are the possible results in mconkeys agar? |
Lactose fermenting colonies can turn pink and non lactose fermenting colonies remain colorless but the agar plate turns yellow. |
|
|
Why would you use the DNA test agar? |
Differential medium. |
|
|
Why would you use a mconkeys agar? |
Selective for gram negative organisms because it inhibits the growth of gram positive. |
|
|
What are the possible results in mconkeys agar? |
Lactose fermenting colonies can turn pink and non lactose fermenting colonies remain colorless but the agar plate turns yellow. |
|
|
Why would you use the DNA test agar? |
Differential medium. |
|
|
Possible results of DNA test agar? |
Positive test: agar around colonies turns pink (DNA has broken down) Negative test: no change in agar color around colonies |
|
|
Why is macconkeys agar both selective and differential? |
It’s selective for gram negative organisms and differential for lactose metabolism. |
|
|
What are the 4 direct methods of measuring microbial growth? |
1. Filtration (pass water through a filter so the filter traps the microbes) 2. The most probably number method (inoculation of tubes of lactose broth with the sample) 3. Direct microscopic count (use the microscope and a special counting chamber) 4. The standard plate count (count the numbers of colonies in a plates after incubation) |
|
|
What are the 4 direct methods of measuring microbial growth? |
1. Filtration (pass water through a filter so the filter traps the microbes) 2. The most probably number method (inoculation of tubes of lactose broth with the sample) 3. Direct microscopic count (use the microscope and a special counting chamber) 4. The standard plate count (count the numbers of colonies in a plates after incubation) |
|
|
What are the indirect methods of measuring microbial growth? |
1. Turbidity (light that shines through the cloudiness in broth culture) 2. Metabolic activity (measure the consumption of a substance required for growth like oxygen) 3. Dry weight (removal of water and weighing the dry material) |
|
|
What are the rules when doing a plate count? |
1. Only plates containing between 30 and 300 colonies can be counted 2. All colonies must be counted |
