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17 Cards in this Set

  • Front
  • Back
Microbial Growth
-binary fission
-growth cycle
-environmental effects on microbial growth
Generation Time (Doubling Time)
the time required for one cell to divide into two cells (one generation)
Process of Binary Fission
-cell elongation
-septum formation
-completion of septum; formation of walls; cell separation
Divisome
responsible for the synthesis of new cytoplasmic membrane and cell wall material
FtsI
needed for peptidoglycan synthesis and its activity is blocked penicillin
Fts Proteins
-key to the processes of cell division & chromosome replication
-interact to form a division apparatus in the cell called the divisome
80 MIN
time it takes for a cell to undergo binary fission
Min
these proteins facilitate FtsZ location of the cell midpoint
MreB Proteins
forms a simple cytoskeleton in cells of bacteria and defines the shape of the cell
-coccoid cells lack this type of gene
New Cell Wall
synthesized during bacterial growth by inserting new glycan units into preexisting wall material
Autolysin
activity creates small openings in the cell wall; new peptidoglycan is synthesized
Bactoprenol
transports peptidoglycan precursors across the cytoplasmic membrane
Transpeptidation
-reaction that leads to the final crossing of two peptidoglycan chains
-inhibited by the antibiotic penicillin
-synthesis of new cell wall stops and the continued action of autolysins leads to cell lysis
-occurs on the outside of cytoplasmic membrane, where ATP is unavailable
Plate Counts (Cell Counts)
measure only the living, reproducing populations
Petroff-Hausser Counting Chamber
method not suitable for counting suspensions of low density or motile cells
-total cell counts measure the total number of cells in a populations
Formation of Biofilms
-attachment: adhesion of a few cells to a suitable solid surface
-colonization: intercellular communication, growth & polysaccharide formation
-development: more growth & polysaccharide
Continuous Culture Device (Chemostat)
population density is controlled by the concentration of limiting nutrient in the reservoir; and the growth rate is controlled by the flow rate
-high dilution rate: cells can't grow fast enough and are washed out