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71 Cards in this Set
- Front
- Back
Spallanzani's conclusion about spontaneous generation was challeneged when?
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Lavosier showed oxygen was essential for life
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Pasteur is credited for all of the follow except?
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Specific microbes cause specific disease in hummans
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The ability of a microscop lens to distinguish between two points is based on it's what?
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Resolution
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Bacteria have a net _____ charge on their surface and are stained with _____ dyes
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Negative, basic
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Following the decoloring step in the gram stain, gram positive cells appear _____ and gram negative cells appear _____.
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Purple, colorless
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Which of the following pairs is mismatched?
a) capsule- simple stain plus negative stain b) cell arrangement-simple stain c) cell size- negative stain d) gram stain-bacterial identification e) none of the above |
E
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Neisseria gonorrhoeae is?
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Gram negative cocci
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Which of the following microscopes requires the specimen be placed in a vacuum?
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Transmission electron
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Special stain is used to?
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Color and isolate various structures such as flagella
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The movement of flagellum is best described as?
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propellar like
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Gram positive cells are more sensitive to antibiotics compared to gram negative cells because gram positive cells do not have what?
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Outermembranes
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There is no immunity to syphilis. This is because the bacteria?
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Has an outer sheath
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Individuals with cystic fibrosis would be most concerned about infection by bacteria with what?
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capsules
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Bacterial membranes can be damaged by what?
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alcohol, detergents, and polymixins
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All are true of endospores except
a) dormant structures b) resistant to UV light, heat, and chemicals c) function as energy reserve inclusions d) germinate into vegetative cells |
C
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Which of the following is not a distinguishing characterisitic of procaryotic cells?
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They lack a plasma membrane
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Which of the following pairs is mismatched?
a) glycocalyx-adherence b) pili-reproduction c) cell wall-toxin d) cell wall-protection e) plasma membrane-transport |
B
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You have isolated a motile, gram-positive cell with no visible nucleus. You can assume this cells has what?
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Ribosomes
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Bacteria that would grow on refrigerated foods are most likely?
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Psychrotrophs
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You inoculated a brother tube and observed bacteria growth only in the bottom of the tube. The oxygen requirment of the bacterium is most likely?
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Obligate anaerobe
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The Term Fastidious refer to bacteria that require?
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organic growth factors
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A chemically defined medium would not contain?
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Beef extract
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Mannitol Salts Agar?
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Differentiates betwen pathogenic and non pathogenic Staphylococcus
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Nutritional studies are done using what type of medium?
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Chemicalled defined
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MacConkey agar is best used to grow samples from patient's with what?
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Diarrheae
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Blood agar is best used to grow samples from patient's with what?
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Sore throat?
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Reducing media is used to culture what?
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Clostridium tetani
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Bacterial populations peak at approximately _____ cells per ml.
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1 billion
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The object of pasteurization on milk is to what?
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kill all milk borne vegatative pathogens
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WHich of the following statements concerning chemicals used in disinfection is not true?
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all kill spores
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Which of the following infectious agents is most sensitive to chemical disinfection?
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Enveloped viruses
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Ethanol would not be effective against what?
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Poliovirus
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Which of the following chemical agents would be effective against both ensospores and mycobacteria?
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Glutaraldehyde
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True or flase, G+ bacteria are more susceptible than G- bacteria to chemical disinfection
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True
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The best method to serilize tissue culture flasks packaged in a plastic bag?
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Gamma rediation
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Avian influenze A?
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Bird Flue
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Staphylococcus aureus?
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Endemic in many hospitals since methicillin resistance
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West Nile Encephalitis?
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Transmitted between birds and to humans by mosquitoes
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Cretzfeldt-Jakob disease?
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Disease caused by prions
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Invasive Group A streptococcus?
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so called flesh eating bacteria
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Centrosomes?
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Organizing center for mitotic spindle
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Peroxisomes?
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Oxigation of amino acids and fatty acids
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Mitochondria?
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Enzymes for ceullar respiration
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Lysozyomes?
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Digest various molecules and bacteria
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Cell wall?
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protection from osmotic lysis
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Endospore?
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Resting
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Fimbriae?
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attachment to surfaces
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Flagella?
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Motility
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Glycocalyx?
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Protection from phagocytosis
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Name the catagory of bacteria that lacks a cell wall?
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Mycoplasm
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Antibiotic that prevents peptidoglycan synthgesis?
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Penicillin
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Name for the types of microorganisms that live in extreme conditions?
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Archea
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Acellular microorganism composed of a DNA or RNA core and protein coat?
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Virus
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Type of sterilization that ensures Clostridium botulinium spores are killed?
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Commericial Sterilization
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Gram negative cellular structure within which endotoxines are located?
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Outermembrane
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Space or area in procaryotic cell within which the DNA is found?
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Nucleoid
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Medium that differientiates between alpha and beta hemolytic Streptococci?
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Blood Agar
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Nutritional term means to use organic compounds as a source of carbon?
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Heterotrophic
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Term for absence of significant contamination?
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Aspesis
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Germ?
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Germs are referred to as a constantly dividing microorganism
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Biofilm?
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Biofilms is a structure that houses many species of cells in one organized community
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Procaryotic?
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A cell without a well defined Nucleus
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Disinfection?
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The removal of vegatative Pathogens
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Gram positive cocci, golden colored, in grape list clusters?
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Staphylococcus Aureus
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Gram negative rod, inhabits the large intestine?
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Escherichia coli
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Spirochetes, causes syphilis?
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Treponema Pallidum
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Attaches to mucous membranes in the genital urinary tract and surface of the eye?
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Neisesseria gonorrhea
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Define Antibiotic?
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Microbial Metabolic with Antimicrobial Acitivity
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Any one of three bacterial test organisms used to evaluate disinfectant
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Escercichia Coli
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Define disinfection. How does antisepsis differ from disinfection? Cite one example of a chemical that can be used as both a disinfectant and an antiseptic.
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See Test 1 Long answer
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Define the term virulence factor and explain why bacterial why bacteria capsules are considered virulence factors?
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Long Answers Test #1
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