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92 Cards in this Set

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Nutrition

Process by which chemical substances called nutrients are acquired from environment and used in cellular activities

Essential nutrient

Element/compound that must be provided to an organism macro

Macronutrient

Required in large quantities

Micronutrient

Required in smaller amounts (iron, zinc, etc)

Organic nutrients

Molecules that contain carbon and hydrogen-- product of living things

Inorganic nutrients

Composed of elements other than carbon or hydrogen-- mineral deposits in the soil, water, or atmosphere

Heterotroph

Organism that obtains carbon in organic form-- dependent on other life forms

Autotroph

Used inorganic carbon dioxide as its carbon source-- converts carbon dioxide into organic compounds-- not dependent on other life forms

Growth factor

Organic compound that cannot be synthesized by an organism and must be provided as a nutrient (cells require 20 amino acids but can't make all so some are obtained through food)

What are the main determinants of a microbe's nutritional type?

It's sources of carbon and energy

Phototrophs

Photosynthesize to get energy

Chemotrophs

Gain energy from chemical compounds

Photoautotrophs

Photosynthetic; converts light energy into chemical energy

Oxygenic photosynthesis

Oxygen-producing (plants, algae, cyanobacteria)

Anoxygenic photosynthesis

No oxygen produced

Chemoautotrophs

Bacteria/archaea that survive on inorganic substances such as minerals and gases

What drives the synthetic process of the cell?

Chemoautotrophs remove electrons from inorganic substrates (hydrogen gas, hydrogen sulfide, sulfur, iron) and combine them with other inorganic substances (carbon dioxide, oxygen, hydrogen) which releases simple organic molecules and energy

Methanogens

Archaea found in extreme habitats-- metabolism adapted by producing methane gas

Chemoorganotrophs

Derive both carbon and energy from organic compounds (majority of heterotrophs)

Chemoheterotrophs

Require organic carbon source

Saprobes

Free living microbes that feed on organic matter released by dead organisms

Symbionts

Derive organic nutrients from bodies of living organisms

Saprobe characteristics

Decomposers of plant litter, animal matter and dead microbes-- can't engulf food, releases enzymes to extracellular environment to digest food particles into smaller molecules that can be transported into cell (bacteria, fungi)

Facultative parasite

Doesn't rely on host for completion of life cycle, but CAN be parasitic

Parasites

Invades the body of a host and uses it as a habitat and source of nutrients and harms the host

Pathogens

Parasitic microbes that grow inside sterile tissues and cause damage/death

Ectoparasites

Live on the body

Endoparasites

Live in organs and tissues

Obligate parasites

So dependent on the host that they can't survive/grow on their own

Diffusion

Net movement of molecules down concentration gradient by random thermal motion-- molecules become dispersed from area of high concentration to area of low concentration which makes them evenly dispersed in solution

Increase in what makes molecules move faster?

Kinetic energy

Passive transport

Does not require energy-- diffuses across the membrane

Osmosis

Diffusion of water through selectively permeable membrane

Isotonic conditions

Environment is equal in solute concentration to cell's internal environment-- diffusion of water proceeds at same rate in both directions so there is no net change in cell volume

Hypotonic conditions

Solute concentration of external environment is low than that of the cell's internal environment-- cells without walls can swell and burst when exposed to this condition

Hypertonic environment

Exposed to solution with higher solute concentration than it's cytoplasm-- forces water to diffuse outside of cell

Plasmolysis

Shrinkage of protoplast in cells without a cell wall

Turbid

Swollen/congested cytoplasmic membrane

Amoeba

Has no cell wall so it must expend extra energy to deal with influx of water-- accomplished with a water vacuole that pushes excess water out of cell

Facilitated diffusion

Transport mechanism that uses carrier protein in membrane that will bind to a specific substance-- changes conformation of carrier protein in a way that facilitates movement of substance across membrane

When is facilitated diffusion used?

When the substance is less permeable and diffusion is unable to transport it

Specificy

Carrier proteins that bind and transport only a single type of molecule

Aquaporins

Water channels; facilitate transport of water molecules following existing osmotic gradient-- regulate volume and osmotic pressure

What must a microbe do to ensure constant supply of nutrients?

Capture nutrients in low concentrations and actively transport them into the cell

Carrier-mediated active transport

Functions with specific membrane proteins that bind ATP and molecules to be transported-- release of ATP drives molecule through protein carrier

Group translocation

Couples transport of a nutrient with its conversion to a substance that's immediately useful inside the cell

Endocytosis

Cell encloses substance in membrane, forming a vacuole and engulfing it

Phagocytosis

Amoebas and certain white blood cells ingest whole cells or large solid matter

Pinocytosis

How liquids enter the cell (oils: molecules in solution)

Minimum temperature

Lowest temp that permits a microbe's growth and metabolism

Maximum temperature

Highest temp that permits growth and metabolism-- any higher can cause denaturation and death

Optimum temperature

Between minimum and maximum temps that promotes fastest rate of growth and metabolism

Psychrophile

Microorganism with optimum temp below 15 degrees C but can grow at 0-- can't grow above 20 degrees C

Mesophiles

Organisms that grow at intermediate temps-- optimum growth temp is 20-40 degrees C-- inhabit animals, plants, soil and water (guardia, bacillus,clostridium)

Thermophile

Microbe that grows optimally at temps greater than 45 degrees C-- live in soil and water

Hyperthermophiles

Grow between 80-121 degrees C

Atmospheric gases that most influence microbial growth

Oxygen and carbon dioxide

Singlet oxygen (1O2)

Produced by phagocytes to kill invading bacteria-- buildup of singlet oxygen can damage and destroy cell

Capnophiles

Grow best at higher carbon dioxide tensions than are normally present in the atmosphere

Neutrophiles

Microorganisms living in soil, fresh water or bodies of plants/animals that live within the range of pH 5.5-8

Acidophiles

Include Eugenia mutabilis and Thermoplasma-- grows in acid pools between 0-1 pH

Alkalinophiles

Live in pools/soils that contain high levels of basic minerals

Syntrophy/cross feeding

Community feeding of organisms sharing a habitat-- products given off by one organism are usable to another

Amensalism

One microbe causes adverse effect in another-- involves competition and occurs where microbes share space/nutrients

Antibiosis

Release of natural chemicals/antibiotics to kill other microbes (fungi and bacteria use this)

Biofilms

Used as adaptive strategy-- favors persistence in habitats and offers greater access to life sustaining conditions

Quorum sensing

Swimming microbes (planktonic) attracted to surface and settle down-- stimulates cells to secrete slimy/adhesive matrix that binds them to substrate, and once attached cells release inducer molecules

Inducer molecules

Accumulate as cell population grows (can monitor size of their own population)-- stimulates expression of particular gene and synthesis of protein product

Binary fission

How bacteria cells replicate-- parent cell splits into 2 daughter cells

Generation time

Time required for complete fission cycle-- average time for bacteria is 30-60 minutes

Chemostat

Continuous culture system-- admits steady stream of new nutrients off used media and old bacterial cells to stabilize growth rate and prevent culture from entering death phase

Cell content

70% water


Proteins


96% CHNOPS

Halophiles

Require high concentration of salt

Osmotolerant

Do not require salt but can tolerate it

Lag phase

Flat period of adjustment, little growth

Exponential phase

Maximum growth

Stationary phase

Nutrients depleted and oxygen gone-- rate of cell growth equal to rate of cell death

Death phase

Cells die as factors intensify

Aerobe

Uses oxygen and can detoxify it

Obligate aerobe

Cannot grow without oxygen

Facultative aerobe

Utilizes oxygen, but can grow without it

Microaerophilic

Require small amount of oxygen

Anaerobic

Does not utilize oxygen

Obligate anaerobe

Lacks enzymes to detoxify oxygen so it can't survive in oxygen environment

Aerotolerant anaerobes

Does not utilize oxygen but can grow in its presence

Symbiotic

Organisms live in close nutritional relationships--required by one or both members

Nonsymbiotic

Relationships not required for survival

Mutualism

Both members benefit

Commensalism

Other member doesn't benefit but not harmed

Parasitism

Parasite dependent and benefits-- host harmed

Synergism

Members cooperate and share nutrients

Antagonism

Some members inhibited/destroyed by others