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6 Cards in this Set
- Front
- Back
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How do uncatalyzed chemical rxns look like on rate vs [substrate] plots?
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linear
-starting rate is linearly proportional to [subsrate] -a 2-fold increase in [subsrate] increases the rate 2-fold |
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How do catalyzed chemical rxns look like on rate vs [subsrate] plots?
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-hyperbolic
-starting rate reaches a max velocity at high [s] -a 2-fold change in [s] does not lead to 2-fold change in rate -enzymes speed up rxns & thus yield more complicated kinetic plots -high [s] the enzyme becomes saturated & a asymptote Vmax is approached |
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What would happen to the asymptote (max rate of rxn) if amount of enzyme present in rxn is cut in 1/2?
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rate is cut in 1/2
-1/2 Vmax |
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What's the equation rate of uncatalyzed rxn?
What's the equation rate of catalyzed rxn? |
rate = k[S]
Michaelis-Menten V=Vmax [S]/([S]+Km) |
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According to Mechaelis-Menten equation, what will happen at high [S]?
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higher [S] = closer to V= closer to 1
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What is V equal to if the [S] is equal to Km?
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1/2Vmax
Km = [S] at which initial velocity V = 1/2 Vmax |
