Mditerm2_Gpr

Front 1

GPR - aerobic, spore forming genus?

Back 1

Bacillus genus
a. B. anthracis
b. B. cereus
c. B. subtilis

Front 2

GPR - anaerobic, spore forming genus?

Back 2

Clostridium genus
a. C. tetani
b. C. botulinum
c. C. difficle

Front 3

GPR - aerobic, NON-acid fast, CATALASE (+) genus? (3)

Back 3

1. Corynebacterium genus
2. Coryneform genus
3. Listeria monocytogenes

Front 4

GPR - aerobic, NON-acid fast, CATALASE (-) genus?

Back 4

Erysipelothrix rhusiopathie

Front 5

GPR -anaerobic, weakly ACID-FAST genus?

Back 5

Actinomyces

Front 6

GPR - anaerobic, NON-acid fast genus? (6)

Back 6

1. Lactobacillus genus
2. Bifidobacterium genus (*)
3. Propionibacterium genus (*)
4. Rothia genus
5. Eubacterium genus
6. Mobiluncus genus

Front 7

Corynebacterium diphtheriae characteristics? (9)

Back 7

1. GPR
2. NON-spore forming
3. NON-acid fast
4. Catalase (+)
5. Polymorphic
6. Metachromic gram stain
7. Babes-Ernst volutin granules
8. NO capsule
9. NO flagella

Front 8

What are C. diphtheriae's 'Babes-Ernst volutine granules'?

Back 8

Polymetaphosporic acid = energy source

Front 9

Specific stain for C. diphtheriae?

Back 9

Neisser Stain

Front 10

Cultivation C. diphtheriae? (2)

Back 10

1. Clauberg medium (hemolysed blood + glycerol+K+-tellurite)
2. Loeffler medium (clotted cattle serum)

Front 11

Clauberg medium (C. diphtheriae) colony TYPES? (4)

Back 11

1. Gravis
2. Mitis
3. Intermedius
4. Belfanti

Front 12

Diphtheroids? (2)

Back 12

1. Normaflora of skin/mucosa
2. Resemble diptheriae bacterium but don't produce the diptheriae toxin and thus, are avirulent, only infect immunosupressed individuals

Front 13

Diphtheriae toxin? (4)

Back 13

1. Exotoxin
2. AB toxin
3. A = ADP-ribosyl-transferase (--> of EF2)
4. B = binding subunit

Front 14

What on in the oropharynx in Pharyngeal diphtheriae?

Back 14

Pseudomembrane (fibrin, WBCs, RBCs, necrotic cells)

Front 15

Pharyngeal diphtheriae symptoms? (5)

Back 15

1. Fever
2. Sore throat
3. Huskiness
4. Heavy breathing
5. bull-neck
(can *asphyxia)

Front 16

Systemic effects: C. diphtheriae? (2)

Back 16

1. DCM (dilative cardiomyopathy)
2. Motoric neuropathy (soft palate mm., mimic mm., eye mm.)

Front 17

Where to take sample for C. diphtheriae? (3)

Back 17

1. Pseudomembrane
2. Ulcer
3. Wound

Front 18

Detection tests: Diphtheriae Toxin? (3)

Back 18

1. ELEK test (antitoxin + sample, precip = (+))
2. Roemer's test (inject guinea pigs, w/+w/out anitoxin)
3. PCR (toxin gene)

Front 19

Immunity test: Diphtheriae toxin? (2)

Back 19

1. Shick's test
a. add heat inactivated shick toxin to skin spots
b. red in 24hr, stay 4d + = NO immunity
c. no rxn or pseudorxn = IMMUNITY
2. ELISE (antitoxin titer)

Front 20

Therapy: C. diphtheriae? (3)

Back 20

1. Antitoxin (give imm!)
2. Penicillin (=elim = prevention)
3. DPT VACCINE (diptheriae, acellular bordetella pertussis components, tetanus toxid)

Front 21

Diphtheroids that are MDR + nosocomial pathogens? (2)

Back 21

1. C. jeikeium
2. C. urealyticum

Front 22

Corynebacterium jeikeium causes? (4)

Back 22

1. Wound infections
2. Sepsis
3. Endocarditis
4. Catheter infections

Front 23

Corynebacterium urealyticum causes? (4)

Back 23

1. Wound infection
2. Sepsis
3. Endocarditis
4. UTIs

Front 24

Listeria monocytogenes? (9)

Back 24

1. GPR
2. aerobic
3. NON-spore forming
4. NON-acid fast
5. Catalase (+)
6. Peritrichous flagella
7. NO capsule
8. B-hemolytic (slightly)
9. Salt resistant

Front 25

Listeria monocytogenes can grow at what temperatures? (3)

Back 25

1. @ RT (~22 C)
2. @ 4 C
3. NOT @ 37 C
(shows cold enrichment)

Front 26

Listeria monocytogenes has a natural resistance to what AB?

Back 26

Cephalosporins

Front 27

Virulence factors: Listeria monocytogenes? (6)

Back 27

1. LLO (listeriolysin-O_
2. MPA (monocytosis producing agent)
3. Act-A
4. PLC
5. Internalin
6. Surface molecuels

Front 28

Infection source: Listeria monocytogenes? (3)

Back 28

CONTAMINATED FOOD
1. Animal products
2. Soft cheese
3. Vegetables

Front 29

What species can pass thru the placenta and cause neonatal infections? (2)

Back 29

1. Treponema spp.
2. Listeria spp.

Front 30

Pregnancy Listeriosis? (4)

Back 30

1. Stillbirth
2. Abortion
3. Premature birth
4. Granulomatosis infanti septica

Front 31

1st causative agent of neonatal meningitis?
2nd?
3rd?

Back 31

1. S. agalactiae
2. E. coli
3. Listeria monocytogenes

Front 32

Adult Listeriosis? (4)

Back 32

1. Meningitis
2. Sepsis
3. Endocarditis
4. Arthritis

Front 33

Listeria monocytogenes diagnostics? (5)

Back 33

1. Cultivate + microscopic examination
2. Sample: stool, blood, food
3. Anton rxn (purulent keratoconjuctivitis in rabbit)
4. Latax agglutination (in cause of meningitis, CSF)
5. Slide agglutination (serotyping)

Front 34

Therapy: Listeria monocytogenes?

Back 34

1. Ampicillin + gentamicin

Front 35

Erysipelothrix rhusiopathiae characteristics? (9)

Back 35

1. GPR
2. aerobic
3. NON-spore forming
4. NON-acid fast
5. Catalase (-)
6. NO flagella
7. NO capsule
8. alpha-hemolytic
9. zoonotic infection

Front 36

Erysipeloid entry and characteristics? (2)

Back 36

1. Entry - hand wounds
2. Painful, skin lesion

Front 37

Other than Erysipelas, what else can E. rhusiopahtiae cause?

Back 37

1. Endocarditis
2. Sepsis
3. arthritis (rare)

Front 38

E. rhusiopathiae:
1. Therapy?
2. Prevention?

Back 38

1. Penicillin G
2. Barriers + swine vaccination

Front 39

Lactobacillus genus: Characteristics? (5)

Back 39

1. long GPR
2. NON-spore forming
3. NON-acid fast
4. Microaerophilic (1 or anaerobic)
5. Vaginal normaflora

Front 40

Lactobacillus genus MEDIUM?

Back 40

Rogasa agar (high [glucose], low pH)

Front 41

Glucose fermentation types? (3)

Back 41

1. Homofermentative (lactic acid)
2. Heterofermentative (lactic acid, acetate acid, CO2, ethanol)
3. Facultative heterofermentative (diff fermentation of glucose, gluconic acid)

Front 42

Doderlein-rod? (Lactobacillus genus) (2)

Back 42

1. Lactic acid production in vagina
2. Purity grades I-II

Front 43

Doderlein-rod: PURITY GRADE I? (3)

Back 43

1. Pure Doderlein-rods
2. Few epithelial cells
3. Few WBCS

Front 44

Doderlein-rod: PURITY GRADE II? (4)

Back 44

1. Few Doderlein rods
2. Other bacteria
3. Few epithelial cells
4. Many WBCs

Front 45

Doderlein-rod: PURITY GRADE III? (3)

Back 45

1. NO Doderlein-rods
2. Pathogens
3. MANY WBCs

Front 46

Pre-biotics?

Back 46

Non-digestible chemicals having beneficial effects on the normaflora of the colon

Front 47

Pro-biotics?

Back 47

Products containing beneficial live organisms

Front 48

Bacterial Vaginosis? (3)

Back 48

1. *Vulvo-vaginitis
2. DUE to lack/loss of LACTOBACILLUS SPP.
3. High [obligate/facultative pathogen]

Front 49

What criteria is used to diagnosis BACTERIAL VAGINOSIS?

Back 49

Amsel criteria

Front 50

Amsel criteria? (4)

Back 50

1. Vaginal pH > 4.5
2. Vaginal discharge (thin, white, yellow)
3. Clue cells
4. (+) Amine test (=add 10% KOH -->*fishy odor = (+))

Front 51

Lactobacillus genus causes what in immunosupressed patients? (2)

Back 51

1. Wound infections
2. Endocarditis

Front 52

Therapy: Lactobacillus genus? (3)

Back 52

1. Penicillin G
2. Vancomycin
3. Metronidazole R (20-30%)

Front 53

Pre-biotics + Pro-biotics makes?

Back 53

Syn-biotics

Front 54

What is the normaflora found in NEONATES during breast feeding?

Back 54

Bifidobacterium genus

Front 55

Bifidobacterium genus characteristics? (4)

Back 55

1. GPR
2. NON-spore forming
3. Anaerobic
4. NON-acid fast