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15 Cards in this Set
- Front
- Back
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Unique features of RNA
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1. Single stranded
2. Ribose (so OH at 2'position) 3. Form stem/loop, hairpins 4. U/G base pairs allowed |
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Svedberg
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rRNA is named by sizes or units
Larger the S value, the higher the MW protein-like, exhibits catalytic activities |
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Parts of tRNA
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D loop (dihydrouracil)
Anticodon loop Variable loop Pseudouracil loop Acceptor stem |
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Prokaryotic mRNA
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Has multiple ORFs Polycistronic
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Eukaryotic mRNA
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Has only one ORF specificing one polypeptide
Also has 5'cap and 3' poly A tail |
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Compare class of RNA with abundance & diversity
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rRNA-most abundant, least diverse
tRNA mRNA-least abundant, most diverse As abundance decreases, diversity increases |
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Unique features of RNA synthesis
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-Requires template, ribonucleotide triP, RNA pol., no primer, no need for proofreading
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Is RNA synthesis conservative, semi-conservative?
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Conservative because DNA duplex reforms
DNA replication is semi-conservative |
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Structure of RNA Pol
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1. Core Pol
2. Holoenzyme + sigma factor (which directs start) |
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During initiation, promoter region where?
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Usually upstream from start
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What does the sigma factor recognize and allows RNA pol to bind?
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Boxes
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What unwinds the DNA strand?
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RNA pol which has helicase activity
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How does termination occur?
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Sees G-C rich hairpin, followed by a string of U's so that the RNA-DNA duplex falls apart
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Antinomycin D
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Blocks unwinding of DNA by RNA pol.
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Rifampicin
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Inhibits RNA pol activity
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