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18 Cards in this Set

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Agar

provides solid matrix to support bacterial growth. contains nutrient mixture of carbohydrates, amino acids, nucleotides, salts, and vitamins

Antibiotic Selection

use of an antibiotic to select bacteria containing the DNA of interest. pGLO plasmid DNA contains the gene for beta-lactamase that provides resistance to the antibiotic ampicillin.

arabinose

a carbohydrate isolated from plants that is normally used as source of food by bacteria

Beta-lacatmase

a protein that provides resistance to the antibiotic ampicillin. The protein is produced and secreted by bacteria that have been transformed with a plasmid containing the gene for beta-lactamase

biotechnology

applying biology in the real world by the manipulation of living organisms, especially at the genetic level, to produce potentially beneficial products

cloning

process of generating virtually endless copies or clones of an organism or segment of DNA (produces a population that has an identical genetic makeup)

colony

clump of genetically identical bacterial cells growing on an agar plate. Because all of the cells in a single colony are genetically identical, they are called clones

culture media

the liquid and solid media referred to as LB, broth and agar are made from an extract of yeast and an enzymatic digest of meat byproducts, all essential nutrients for bacterial growth

genetic engineering

manipulation of an organism's DNA by introducing or eliminating specific genes

gene regulation

gene expression in all organisms is carefully regulated to allow for differing conditions and to prevent wasteful overproduction of unseeded proteins

green fluorescent protein

originally isolated from bioluminescent jellyfish. The gene for GFP has been cloned. The unique three dimensional conformation of GFP causes it to resonate when exposed to UV light and give off energy in the form of visible green light

plasmid

a circular DNA molecule, capable of self replicating, carrying one or more genes for antibiotic resistance proteins and a cloned foreign gene such as GFP

pGLO

plasmid containing the GFP sequence and ampicillin resistance gene, which codes for beta-lacamase

recombinant DNA technology

process of cutting and recombining DNA fragments as a means to isolate genes or to alter their structure and function

screening

process of identifying wanted bacteria from a bacterial library

sterile technique

minimizing the possibility of outside bacterial contamination during an experiment through observance of cleanliness and using careful lab techniques

streaking

process of passing an inoculating loop with bacteria on it across an agar plate

vector

an autonomously replicating DNA molecule, such as a plasmid, into which foreign DNA fragments are inserted and then propagated in a host cell