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22 Cards in this Set

  • Front
  • Back

desriptive embryology?

Describe what is happening inside and outside



developing fate maps, detailing the destiny of each cell



catalouging gene expression, when and where genes are being expressed



no or little interferrring with the process

experimental embryology?

work out how and why everything is happening



experiments to find how development occurs



includes transplant studies to find whether cells or tissues obtain their fates from neighbours or determinants

what is the common labelling method

blue= ectoderm ( outside of cell)



red= mesoderm (formed during gastrulation)



yellow = endoderm (inside of cell)

What are thought to be the methods that the fate of cells are determined?

1) Inheritance of cell fate determinants



( Aysmmetrical inheritance of cytoplasmic determinants)



2) Cell-cell communication / induction

How did dropsphilia embryos indicate mosaic development?

their egg cells are already assymetrical



studies showed how determinants in portions of the cytoplasm determine cell fate

how does one find out how much of a role cell-fate determinants play in dertmining how cells develop?

compare a fate map to a specification map



fate maps are produced from following cells and their daughters fates etc



specification maps are developed by removing cells from the embryo, this isolates them from their environment,



what the isolated cells develop into provides a specification map

general differences between mosaic and regulatory devlopment

mosaic development, if cell is removed, what that cells fate would have been is removed (cell determinants)



regulatory development, can adapt from being removed ( cell-cell communication)

how could you prove regulatory devlopment?

cells that

stages of frog development?

blastula



gastrula: gastrulation



neurula: neural tube forming



tailbud



tadpole/ prelarval

what are the cells of blastula called?

blastopore

morphogenesis?

process by which form is generated, involves coordinated cell movements

gastrulation?

morphogentetic process in which the endoderm, mesoderm and ectoderm layers reach their final positions in the embryo

neuralation?

morphogenetic process in which the nervous system begins to develop, formation of neural tube

fate map?

assesement of fate of a cell or group of cells based on lineage labelling



part of distriptive embryology, as it does not involve interupting normal functioning,

specification map?

assesment of what a cell or group of cells will form if removed from their embryonic environment

what is it where a cell is 'determined,'?

a cell or tissue that is determined if, when it is removed or transplanted it will still develop accordingly



example dorsal lip of blastopore (organiser) will still determined to become a notochord even if it is transplanted to ventral side of embryo

what is it where cell speciation map is not same as fate map?

indicates that some cells must have receive signals from neighbours (cell-to-cell) at a later stage of development that determine their fate

competence?

competence is the range of cell fates, which can be achieved by a cell or a group of cells in a given set of conditions



Induction?

the process in which cell or group of cells emit signals to its neighbouring cells, thereby changing its fate



example is the induction of neural ectoderm by dorsal mesoderm in organizer exp

breifly state what happens in the organizer experiment?

induction of muscle (somites) and neural tissue by the transplanted dorsal mesoderm



the dorsal mesoderm that was transplanted onto the ventral side of the embryo is already determined by early gastrula stage



the fact that the ventral ectoderm and mesoderm can develop into neural and muscle tissue means they are competent to become then

what is the organiser region of the gastrula?

it is the dorsal mesoderm at the gastrula stage of the embryo



it induces overlying ectoderm to become neural



and neighbouring mesoderm to become muscle

what is the nieuwook centre?

region of the early blastula stage which induces the organiser