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5 Cards in this Set
- Front
- Back
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what did you use as a blank for this experiment
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cubette for this reaction (30 ml buffer, 10 ml .05 h202 4ml d h20
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what is the substance being measured in this experiment
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HgCl2
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why can you use the absorbance as the rate of the reaction in your lineweaver-burke plots intease of measruing rate as millimoles product per minute
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bc of beers law, absorption is directly proportial to concentration
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would you predict that inhibition of the potato peroxidase enzme by mercuric chloride would be a competitive or non competitive mechanism/ eplain
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non-competitve, bc no structural similarity
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in theory double reciprocal plots of reaction rate vs. substrate concentration with and without inhibitor should cross at either y or x axis.if these lines do ont cross at an axis, what could be a reason
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there is no enzyme competion on the active site
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