Clbact [1-29-24 Quiz]

Front 1

Appears as partially acid fast

Back 1

Nocardia

Front 2

Appears as a gram ghost after gram staining

Back 2

Mycobacterium

Front 3

Acid fast staining technique bacterias

Back 3

* Mycobacterium

* Cryptosporidium

* Isopora

* Cystospora

* Nocardia

* Legionalla micdadei

* Rhodococcus specie

Front 4

Primary stain (Acid-fast) [AF:NAF]

Back 4

Carbol fuschin [Red:Red]

Front 5

Mordant (Acid-fast) [AF:NAF]

Back 5

Tergitol/Physical steam [Red:Red]

Front 6

Decolorizer (Acid-fast) [AF:NAF]

Back 6

Acid alcohol (0.5-1% or 3%) [Red:Colorless]

Front 7

Secondary stain (Acid-fast) [AF:NAF]

Back 7

Methylene blue / Malcchite green [Red:Blue/Green]

Front 8

Acid fast bacilli stain technique can be fixed by with your?

Back 8

Slide warmer at 65C for 2hours

Front 9

Mordant (ZN&K)

Back 9

Physical heat/steam : Tergitol

Front 10

Decolorization (ZN&K)

Back 10

Acid alcohol (0.05-1% or 3%) : **SAME**

Front 11

Primary stain (ZN&K)

Back 11

Carbol fuschin

Front 12

"Hot method"

Back 12

Ziehl-Neelsen

Front 13

"Cold method"

Back 13

Kinyoun

Front 14

Acid fast organism in tissues

Back 14

Kinyoun

Front 15

Uses HNO3-alcohol or H2SO4-alcohol as decolorizer

Back 15

Modified kinyoun

Front 16

Differentiates Mycobacterium smegmatis(-) from Mycobacterium tuberculosis(+)

Back 16

Pappenheim's Method

Front 17

Differentiates Mycobacterium leprae (+) from Mycobacterium tuberculosis (-)

Back 17

Baumgarten Method

Front 18

Selective for the cell wall of AFB

Back 18

Auramine-rhodamine Method

Front 19

Special Stains

Back 19

1. Capsule

2. Flagella

3. Metachromatic Granules

4. Spores

5. Glycogen vacuoles

6. Mitochondria

7. Spirochetes

Front 20

Give some stains under Capsule stain

Back 20

1. Hiss stain

2. Tyler capsule

3. Welch's capsule staling

4. Grin's stain

5. Anthony's stain

Front 21

Give some stains under Flagella

Back 21

1. Leifson stain,

2. Gray's method

3. Caesares Gil method

4. Loeffler's method

5. Van Ermenger Method

6. Fisher and Cohn method

Front 22

Give some stains under Metachromatic granules

Back 22

1. Albert

2. Neisser

3. Ponder

4. Ljubensky method

5. Loeffler's alkaline methylenee blue (LAMB)

Front 23

Give some stains under Spores

Back 23

1. Schaeffer-Fulton

2. Heartr and acetic acid method

3. Dorner's spore stain

4. Wirtz method

Front 24

Give some stains under Glycogen values

Back 24

Iodine or periodic acid schiff (PAS)

Front 25

Give some stains under mitochondria

Back 25

Janus Green B

Front 26

Give some stain under Spirochetes

Back 26

Fontana-Tribondeau

Front 27

Motility

Back 27

1. Vibrio

2. Listeria

3. Mycoplasma

4. Proteus mirabilis & Protees vulgaris

Front 28

Motile bacteria

Back 28

1. Alcaligenes

2. Bacillus cereus

3. Bacillus subtilis

4. Campylobacter

5. Corynebacterium

6. Clostridium botulinum

7. Clostridium tetani

8. Escherichia coli

9. Listeria

10. Proteus

11. Providencia

12. Pseudomonas

13. Salmonella

14. Vibrio

Front 29

Non-motile bacteria

Back 29

1. Bacillus antrax

2. Bordetella

3. Brucella

4. Clostridium perfringens

5. Corynbacterium diphtheriae

6. Erysepelothrix

7. Hemophilus

8. Klebsiella pneumoniae

9. Mycobacterium tuberculosis

10. Neisseria

11. Pasturella

12. Shigella

13. Staphylococci

14. Streptococci

Front 30

With inclusion bodies bacteria

Back 30

1. Corynebacterium

2. Mycobacterium

3. Nocardia and Actinomyces

4. Pasteurella & Bordetella

Front 31

Baterias with capsule

Back 31

1. Bacillus anthracis

2. Klebsialla pneumoniae

3. Pasturella

4. Streptococcus pneumoniae

Front 32

Bacterias with spore

Back 32

1. Bacillus

2. Clostridium

Front 33

Bacterias with pili

Back 33

1. Neisseria

2. Pseudonomas

Front 34

Indirect approaches

Back 34

1. Electrophoretic mobility

2. Antibodies

3. Immunologic

4. Enzymes

Front 35

Determine amino acid sequence of proteins with the same fuction

Back 35

Direct approach

Front 36

Relationships at species and subspecies level

Back 36

Electrophoretic mobility

Front 37

Can discriminate between very similar proteins

Back 37

Antibodies

Front 38

Compare proteins from different microorganinsms

Back 38

Immunologic

Front 39

Reflects amino acid sequence

Back 39

Enzymes

Front 40

Darting motility

Back 40

Vibrio

Front 41

Tumbling motility

Back 41

Listeria

Front 42

Gliding motility

Back 42

Mycoplasma

Front 43

Swarming motility

Back 43

Proteus mirabilis & Proteus vulgaris

Front 44

reflects the base sequence

Back 44

G+C content

Front 45

Determined from melting temperature

Back 45

G+C content

Front 46

The greater the G+C content, the higher the ____?

Back 46

Melting point

Front 47

The use of _____ for Nucleic acid base with a wavelength of ____?

Back 47

Spectrophotometer : UV light with 260nm

Front 48

Why is 70% alcohol more efficient in killing bacteria than 90%

Back 48

Because it contains 30% water (water kill proteins present in the bacteria)

Front 49

If a mixture of single-stranded DNA formed by heating dsDNA is cooled and held at a temperature about 25C below Tm

Back 49

Nucleic acid hybridization

Front 50

While non-compementary strands will remain ____

Back 50

single

Front 51

are the most ideal for studies of microbial evolution and relatedness

Back 51

rRNA

Front 52

Replication of genetic information

Back 52

1. Unwinding

2. Unzipping

3. Synthesis of new DNA

4. Termination of Replication

Front 53

for enzymes and cofactors to act on the DNA molecules

Back 53

unwinding

Front 54

DNA polymerase opens the replication fork

Back 54

Unzipping

Front 55

Each parents strand serves as a template from which a complementary strand is produced

Back 55

Sythesis of the new DNA

Front 56

Occurs when 2 replication forks meet

Back 56

Termination of replication

Front 57

Okazaki fragments

Back 57

Termination of replication (3' to 5')