Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
20 Cards in this Set
- Front
- Back
|
Antigen is particulate (cell or particle) |
Agglutination Aglutenogen is the antigen Aglutinin is the antibody |
|
|
Slide agglutination |
Qualitative test Serological identification |
|
|
Agglutination tube |
Quantitive test Serological diagnosis |
|
|
Pregnancy test |
Passive aglutimation Anti HCG e' latex Mixed e' drop of urine may contain HCG |
|
|
Detection of c reactive ptn |
Passive agglutination C reactive ptn antibody with latex binds to c reactive ptn in serum |
|
|
Rhematic artherites |
Passive agglutination Latex e' IgG binds e'IgM in patients serum |
|
|
Rhematic fever |
Passive agglutination Latex on streptolysin O antigen binds to ASO antibodies in patient's serum يفضل ان ال test ينعمل quantitive لان اغلب الناس عندها ASO antibodies ناتج عن strept pyogenes infection فبنحط standered هو ال 200 unite لل ASO antibody لو اعلى من هيك بشخص. |
|
|
Direct coombs test |
(Anti human globulin ) binds to (anti RH antibody on fetus RBCs) Lead to agglutination كان ال antigen هو الanti RH antibody وال known antibody هو ال anti human globulin والشيال هي ال fetus RBCs |
|
|
Indirect coombs test |
+ve RH RBCs are added to the mother's serum then anti human globulin is added Agglutination means presence of anti RH antibodies in mother serum No agglutination means abcense of anto RH antibodies in mothers serum |
|
|
Heamagglutination inhibition test |
Serum is taken from the patient We add the virus to the serum
Then we add blood No agglutination ▶️ binding of hemaglutinins of patient's serum with the virus
Agglutination denote denotes abcense of hemaglutinins |
|
|
Agar gel |
بستخدمه عشان يحصل presepetation لان لو استخدمت slide او tube فيه soluble antigen وحطتله soluble antibody وارد جدا يحصل zone phenomenon ويكونو soluble immune complexes بدل presepetation |
|
|
Elek's test |
Double diffusion test Qualitative test *Colonies of diphtheria are izolated in straight line *filter paper contain antitoxin against diphtherial toxins is putted to form + shape with the diphtherial colony in the cultre *antitoxin diffuse and the diphtherial toxin will diffuse (double diffusion) To meet in optimum consuntration and presepetate (+ve elek's test) *no presepetation denotes abcense of diphtherial toxins (-ve eleks test) |
|
|
Single radial immunodiffusion |
Quantitative test *agar in its liquid state is mixed with antibody for C3 and leave it to pour in the plate *serum is punched in tge agar gel *if C3 is present in the serum it will diffuse till it reach a obtimum consuntartion of anti C3 antibody The make a radial presepetation *it is quantitative test as we use standers of known quantity of C3 and the we compare it with the ring of the patient's serum C3
نفس الكلام بعمله مع ال IgG بالحاله هاي IgG حيكون هو ال antigen وحستخدم Anti human glubulin |
|
|
Anti human globiulin 🧠T |
Direct coomps test Indirect coomps test Single radial immunodiffusion Indirect immunoflurescence Indirect ELISA Weatren blot |
|
|
Complement fixation test |
Qualititive test مثلا ال syphilis ٥ حيوانات بحط بال tube الآتي ١- بجيب serum من الحيوان الي عنده syphilis هو ال antibody ٢- بجيب antigen من ال heart of beef ٣-بجيب complement من ال ginea pig *بحط indicator system عباره عن ٤-ال RBCs وال antigen تبعها من sheep ٥- ال antibody الي ضد ال RBCS بجيبه من rabbit serum
لو ال RBCs تكسرت يعني ال complement راح بال antigen antibody rection يعني ال test +ve
طبعا ال complement بكون محطوط ب standered يكفي reaction واحد |
|
|
Direct immunoflorescent |
As in rabies virus ١-بجيب الكلب ب section in brain كمصدر لل rabies virus ٢-بحط عليه antibody مربوط ب florecin ٣-بعمل WASH ٤- بضوي ب UV لو نور معناته ال rabies موجود عند الكلب |
|
|
Indirect immunoflurecence |
بدي اشوف لو في عند العيان antibody ضد ال treponema ١-بحط ال treponema على slide ٢-بجيب serum العيان وبحطها عليها ٣-بجيب anti human glubulin محطوط عليه ال florecen ٤- بعمل WASH ٥-بحطه تحت ال uv light لو ضوى معناته انو ال antibody موجود فهو عنده sephilis |
|
|
Direct ELISA |
Quantitive test مثلا بدي احدد كمية ال T3 ١-بجيب antibody ضد ال T3 بقسم الكميه نصين نص بحطه ب tube بروح لازق ال Fc portion عال tube والايدين تبعته جاهزه لاستقبال ال T3 النص التاني بشبكه مع enzyme وبحطه بالتلاجه ٢-بجيب الserum تاع العيان وبحطه بالانبوبه ٣-بعمل WASH 4-بجيب النص التاني من ال antibody الي بالتلاجه بحطه بالانبوبه وبستنى ١٠ دقايق يلزقو بال T3 ٥- بعمل WASH ٦-بحط substrate للانزيم كل ما اللون طلع غامق اكتر كل ما ال T3 الي كانت شابكه اكتر |
|
|
Indirect ELISA |
Quantitive test مثلا بدي اشوف لو العيان عنده antibody ضد ال treponema ١-بجيب ال treponema بالانبوبه ٢- بحط عليها ال serum ٣-بعمل WASH ٤- بحط anti human globulin بلزق فيه enzyme ٥- بعمل WASH ٦-بحط ال substrate كل ما اللون اغمق كل ما كان عند العيان antibody اكتر |
|
|
Radioimmunoessay RIA |
Quantitive test مثلا بدي اقيس كمية ال T3 ١-بجيب ال antibody ضد ال T3 ٢-بحط ال serum ٣- بعمل WASH ٤- بحط ال radioactive T3 كل ما تخفف اللون كل ما يعني ان ال radioactive T3 قلت كل ما يعني ان العيان عنده T3 اكتر |
