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15 Cards in this Set
- Front
- Back
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cultured cells endycytose solutes and particles from their extracellular medium at high rates
know two example of such cells |
fibroblasts and macrophages
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what is the general endocytic pathway
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early endosome late endosome lysosomes
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what is pinocytosis or cell drinking?
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the mechanism by which a cell incorporates fluids or particle less than 5 microns, from the external environment inside the cell through INVAGINATION OF THE CELL MEMBRANE
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the process of pinocytosis requires energy from _____
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adenosine triphosphate
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pinocytosis is used primarly for absorption of ____, and it generates _____
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extracellular fluids ECF and it generates very small vesicles
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what is the primary different of pinocytosis and phagocytosis ?
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pinocytosis is non specific in the substances that it transports. the cell takes in surrounding fluids, including all solutes present
phagocytosis is specific in the substances it transports |
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phagocytosis actually engulfs _____, which are later broken down by _____ and finally _____
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whole particles
enzymes such as lysosomes absorbed into the cells |
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pinocytosis is when a cell engulfs already _____ or ____ food
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dissolved or broken down
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what is horseradish peroxidase?
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an enzyme used to label antigens and their antibodies
belongs to peroxidase groups of enzymes extracted from horse radish plant |
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what is a peroxidase?
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any group of enzymes that catalyze the oxidation of a compound by a peroxide
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the objective of the lab was to label _____ with a soluble marker ___ to observe ___
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labeling the endocytic compartment with soluble marker (HRP) to observe the rate of pinocytosis
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what is the purpose of enzyme assay?
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to determine the internalized marker HRP to observe the rate of pinocytosis
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A1 fluid phase marker HRP = uptake in ____ cells
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fibroblast cells
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name the seven materials we used
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macrophage cell monolayers in plate
alpha minimal essential medium bovine serum albumin (BSA) phosphate buffered saline PBS cell scrapper triton X |
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what is PBS used for
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help to maintain a constant pH. The osmolarity and ion concentrations of the solution usually match those of the human body (isotonic).
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