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3 Cards in this Set
- Front
- Back
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Bacterial Morphology + Cell Wall Struct |
SIZE + SHAPE Prokaryotes 0.2-> 700µm diameter, eukaryotes 10->200µm Shapes - coccus, rod, spirillium, spirochete, appendages, filamentous shape determining factors e.g. MreB, Crescetin CELL WALL STRUCT Gram +ve - 90% peptidoglycan, teichoic acids Gram -ve - 10% peptidoglycan, porins, periplam (gel like consistency 15nm wide), LPS layer (contains polysaccharides) Peptidoglycan-rigid -> provides strength
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Protein Translocation |
what? Proteins crossing cyto membrane either by Sec or Tat pathway SEC PATHWAY (general secretion) Signal Seq: n, h and c region tripartite struct ~20 aminos long SecYEG - spans membrane + stimulates Sec A ->Drives translocation Sec B - secretion spec chaperone maintains Sec substrates in unfolded state while delivering them to Sec A for translocation SRP - signal recognition particle ->binds signal seq -> targets FtsY for translocation at SecA TAT PATHWAY (Twin arginine translocase) Signal seq: n,h,c regions tripartite struct variable length TatABC complex - A (forms pore), B (signal recognition), C (recruits + binds Tat A, translocation motor) Proofreading - by TMAO reductase + hydrogenase SlyD+DnaK = chaperones -> prevent degredation + mistargerting to seq! |
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Bacterial Motility |
Flagella Construction: Basal bod + rings - anchor it to envelope hook complex - connects basal bod to filament Motor - drives flagellar rotation secretion system - exports subunits ATPase energises system Flagellar Synth Regu: Early genes - control expression of regulation middle genes - control expression of struct components Late genes - filament, motor force generators Swimming Motility: Peritrichious prokaryotes - move slow in straight line polar prokaryotes - rapid + spinning Chemotaxis Regu molecules: -CheA, Y, Z, R, B, W Orgaisms that move w/o flagella: -myococcus, neiseria, pseudomonas, cytophoga, flavobacterium - by slime excretion, pili, sliding specific proteins! Requires surface contact!!! |
