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9 Cards in this Set
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Carbohydrate Fermentation Test (purpose, medium, substrate, enzyme, end product, pH indicator, results) |
Purpose: to determine the ability of a microorganism to degrade and ferment carbohydrates with the presence Medium: Carbohydrate fermentation broth with Durham tube Substrates: lactose, sucrose dextrose Enzyme: N/A End products: lactic acid or lactic acid & gas pH indicator: phenol red in medium Neg. result: no color change (red), no gas Pos. result: color change to yellow/gas |
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Indole Production Test (IMViC) (purpose, medium, substrate, enzyme, end product, pH indicator/reagent, results) |
Purpose: to determine the ability of microorganisms to degrade tryptophan Medium: SIM agar deep Substrate: tryptophan enzyme: tryptophanase end product: Indole pH indicator: Kovac's 10 drops Neg. result: brown reagent layer Pos. result: red reagent layer |
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Methyl Red Test (purpose, medium, substrate, enzyme, end product, pH indicator/reagent, results) |
Purpose: to determine the ability of microorganisms to ferment glucose with the production and stabilization of high concentrations of acid end production. Medium: MR-VP broth Substrate: Glucose Enzyme: N/A End Product: Acid pH indicator/reagent: Methyl Red 10 drops Neg. result: yellow Pos. result: red
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Vogues-Proskauer Test (purpose, medium, substrate, enzyme, end product, pH indicator/reagent, results) |
Purpose: differentiate further among enteric organisms (E.coli, E.aerogenes, K.pneumoniae) Medium: MR-VP broth Substrate: Glucose Enzyme: N/A End Product: neutral/non acidic pH indicator/reagent: VP-A & VP-B 10 drops Neg. result: clear, no color change Pos. result: Red |
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Citrate Utilization Test (purpose, medium, substrate, enzyme, end product, pH indicator/reagent, results) |
Purpose: differentiate among enteric organisms on the basis of ability to ferment citrate as a sole source of carbon. Medium: Simmon's citrate agar slant Substrate: Citrate Enzyme: Citrate Permease and Citrase End Product: Sodium Carbonate (alkaline) pH indicator/reagent: Bromythmol blue Neg. result: no color change, green Pos. result: Royal blue color |
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Hydrogen Sulfide Test (purpose, medium, substrate, enzyme, end product, pH indicator/reagent, results) |
Purpose: Motility and ability of microorganisms to produce hydrogen sulfide from sulfur-containing amino acids or inorganic sulfur compounds Medium: SIM agar deep Substrate: Cysteine, Thiosulfide Enzyme: Cysteine Desulferase, Thiosulfate Reductase End Product: Hydrogen Sulfide gas+ Ferrous Ions = FeS pH indicator/reagent: N/A Neg. result: No black, no motility Pos. result: Black, motility= growth away from stab line |
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Urease Test (purpose, medium, substrate, enzyme, end product, pH indicator/reagent, results) |
Purpose: determine ability of microorganism to degrade urea by means of enzyme urease Medium: Urea broth Substrate: Urea Enzyme: Urease End Product: Ammonia pH indicator/reagent: Phenol red Neg. result: no color change Pos. result: hot pink color |
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Nitrate Reduction Test (purpose, medium, substrate, enzyme, end product, pH indicator/reagent, results) |
Purpose: determine ability of microorganisms to reduce nitrates to nitrites or beyond the nitrite stage. Medium: Nitrate reduction broth w/ Durham tube Substrate: Nitrate, Nitrite Enzyme: Nitrate Reductase End Product: Nitrite, N2, or NH3 pH indicator/reagent: Nitrate A & B, Zinc Neg. result: No color change, red Pos. result: red, unchanged |
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Catalase Test (purpose, medium, substrate, enzyme, end product, pH indicator/reagent, results) |
Purpose: determine ability of microorganisms to degrade hydrogen peroxide by producing catalase. Medium: TSA slant Substrate: Hydrogen peroxide Enzyme: Catalase End Product: Oxygen pH indicator/reagent: Hydrogen Peroxide, 3 drops Neg. result: no bubbles Pos. result: bubbles |
