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9 Cards in this Set

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Carbohydrate Fermentation Test


(purpose, medium, substrate, enzyme, end


product, pH indicator, results)

Purpose: to determine the ability of a


microorganism to degrade and ferment carbohydrates with the presence




Medium: Carbohydrate fermentation broth with Durham tube




Substrates: lactose, sucrose dextrose




Enzyme: N/A




End products: lactic acid or lactic acid & gas




pH indicator: phenol red in medium




Neg. result: no color change (red), no gas




Pos. result: color change to yellow/gas





Indole Production Test (IMViC)


(purpose, medium, substrate, enzyme, end


product, pH indicator/reagent, results)

Purpose: to determine the ability of


microorganisms to degrade tryptophan




Medium: SIM agar deep




Substrate: tryptophan




enzyme: tryptophanase




end product: Indole




pH indicator: Kovac's 10 drops




Neg. result: brown reagent layer




Pos. result: red reagent layer

Methyl Red Test


(purpose, medium, substrate, enzyme, end


product, pH indicator/reagent, results)

Purpose: to determine the ability of


microorganisms to ferment glucose with the production and stabilization of high


concentrations of acid end production.




Medium: MR-VP broth




Substrate: Glucose




Enzyme: N/A




End Product: Acid




pH indicator/reagent: Methyl Red 10 drops




Neg. result: yellow




Pos. result: red


Vogues-Proskauer Test


(purpose, medium, substrate, enzyme, end


product, pH indicator/reagent, results)

Purpose: differentiate further among enteric organisms (E.coli, E.aerogenes, K.pneumoniae)




Medium: MR-VP broth




Substrate: Glucose




Enzyme: N/A




End Product: neutral/non acidic




pH indicator/reagent: VP-A & VP-B 10 drops




Neg. result: clear, no color change




Pos. result: Red

Citrate Utilization Test


(purpose, medium, substrate, enzyme, end


product, pH indicator/reagent, results)

Purpose: differentiate among enteric


organisms on the basis of ability to ferment citrate as a sole source of carbon.




Medium: Simmon's citrate agar slant




Substrate: Citrate




Enzyme: Citrate Permease and Citrase




End Product: Sodium Carbonate (alkaline)




pH indicator/reagent: Bromythmol blue




Neg. result: no color change, green




Pos. result: Royal blue color

Hydrogen Sulfide Test


(purpose, medium, substrate, enzyme, end


product, pH indicator/reagent, results)

Purpose: Motility and ability of


microorganisms to produce hydrogen sulfide from sulfur-containing amino acids or


inorganic sulfur compounds




Medium: SIM agar deep




Substrate: Cysteine, Thiosulfide




Enzyme: Cysteine Desulferase, Thiosulfate


Reductase




End Product: Hydrogen Sulfide gas+ Ferrous Ions = FeS




pH indicator/reagent: N/A




Neg. result: No black, no motility




Pos. result: Black, motility= growth away from stab line

Urease Test


(purpose, medium, substrate, enzyme, end


product, pH indicator/reagent, results)

Purpose: determine ability of microorganism to degrade urea by means of enzyme urease




Medium: Urea broth




Substrate: Urea




Enzyme: Urease




End Product: Ammonia




pH indicator/reagent: Phenol red




Neg. result: no color change




Pos. result: hot pink color

Nitrate Reduction Test


(purpose, medium, substrate, enzyme, end


product, pH indicator/reagent, results)

Purpose: determine ability of microorganisms to reduce nitrates to nitrites or beyond the


nitrite stage.




Medium: Nitrate reduction broth w/ Durham tube




Substrate: Nitrate, Nitrite




Enzyme: Nitrate Reductase




End Product: Nitrite, N2, or NH3




pH indicator/reagent: Nitrate A & B, Zinc




Neg. result: No color change, red




Pos. result: red, unchanged

Catalase Test


(purpose, medium, substrate, enzyme, end


product, pH indicator/reagent, results)

Purpose: determine ability of microorganisms to degrade hydrogen peroxide by producing catalase.




Medium: TSA slant




Substrate: Hydrogen peroxide




Enzyme: Catalase




End Product: Oxygen




pH indicator/reagent: Hydrogen Peroxide, 3 drops




Neg. result: no bubbles




Pos. result: bubbles