Ascp Practice Test #3

Front 1

If an Rh group (DCe/dce) man marries an Rh group (dce/dce) woman, what is the probablitiy that their first child will be D-negative?

Back 1

50%

Front 2

What is the most common technique that is used by clinical laboratories for therapeutic drug monitoring?

Back 2

Immunoassay

Front 3

What would be considered a normal cerebrospinal fluid glucose if the serum glucose is 70 mg/dl?

Back 3

45 mg/dL

Front 4

Antigen processing is primarily accomplished by which cell type?

Back 4

Macrophages

Front 5

The temperature range for maintaining red blood cell units (or whole blood units) during shipping is what?

Back 5

1 - 10 ºC

Front 6

What should the diastolic pressure be in a potential blood donor?

Back 6

No higher than 100 mm Hg.

Front 7

Which parasite migrates through the circulation (blood) before maturing in the portal venous system?

Back 7

Schistosoma mansoni

Front 8

Squamous epithelial cells

Back 8

Large, flat, irregular cells with a low nuclear to cytoplasmic ratio are generally found in the urine of females as a result of vaginal contamination

Front 9

Factor VII

Back 9

Stable factor

Front 10

Factor VIII

Back 10

Anti-hemophiliac factor

Front 11

Factor IX

Back 11

Christmas factor

Front 12

Factor X

Back 12

Stuart factor

Front 13

Rocky Mountain spotted fever is transmitted by the bite of a tick infected with:

Back 13

Rickettsia rickettsii

Front 14

Acute phase proteins generally fall into which category?

Back 14

Glycoproteins

Front 15

Oxidase-negative

Non-lactose fermenting

Nonmotile

Gram-negative rod

Back 15

Shigella species

Front 16

What shapes can be used to describe cocci?

Back 16

Round

Lancet

Ovoid

Front 17

Traditional coagulation assays are based almost solely on what technique?

Back 17

Clot-based assays

Front 18

What should be done with a box of 10 Red Blood Cell units that was received with a measured temperature inside the box at 15ºC.

Back 18

Quarantine and destroy the products.

Front 19

Coarse basophilic stippling can be found in which conditions?

Back 19

Megaloblastic anemias

Thalassemias

Lead poisoning

Front 20

What are markers of myocardial necrosis but are not widely measured in evaluating chest pain and ACS due to the labor-intensive method that is needed to measure them?

Back 20

CK-MB isoforms

Front 21

Lactose fermenters

Back 21

Will produce a pink-colored pigment on MacConkey agar

Front 22

The antibody MOST frequently present in systemic lupus erythematosus (SLE) is directed against what?

Back 22

Nuclear antigen

Front 23

Beer's law

Back 23

Based on the fact that absorbance is directly proportional to the concentration of a solution. Therefore, stray light can alter the absorbance results in this type of assay

Front 24

What is true about assays for the antigenic detection of both C. difficile toxins A and B?

Back 24

The assays are rapidly and easily performed & can detect both Toxin A+B- and Toxin A-B+ strains

Front 25

For what reason would a molecular method be used to determine a pregnant woman's Rh type?

Back 25

To differentiate between weak D or partial D

Front 26

In the hemoglobin methodology using potassium ferricyanide what measurable reaction occurs?

Back 26

Ferrous iron is oxidized to ferric iron to form methemoglobin

Front 27

The size of the zone of suppressed growth on a sensitivity plate using sensitivity disks is referred to as:

Back 27

The zone of inhibition

Front 28

Chediak-Hegashi syndrome

Back 28

Abnormal fusion of cytoplasmic lysozymes in granulocytes and occasionally monocytes

Associated with albino characteristics, photophobia, and frequent infections

Front 29

Which chromosome demonstrates a partial or full gene loci deletion in various forms of beta thalassemia?

Back 29

Chromosome 11

Front 30

What is successful molecular identification of methicillin-resistant Staphylococcus aureus (MRSA) based upon?

Back 30

SIMULTANEOUS detection of the S. aureus orfX gene and SEVERAL variants of the mecA gene.

Front 31

Which type of lipoprotein transports the MAJORITY of cholesterol to cells for steroid hormone synthesis or cell membrane incorporation?

Back 31

Low density lipoproteins

Front 32

Which enzyme, produced by Staphylococcus aureus is responsible for preventing penicillin antibiotics from being effective against a Staph infection?

Back 32

Beta-lactamase

Front 33

What is the primary target of HIV?

Back 33

Helper T-cell lymphocytes

Front 34

If a test is said to have a sensitivity of 95%, it will:

Back 34

Miss 5 out of 100 true positives

Front 35

What laboratory test result is commonly used to monitor oral anticoagulant therapy?

Back 35

Prothrombin time (PT)/INR

Front 36

Which immune elements are involved in a POSITIVE TB skin test?

Back 36

T cells and macrophages

Front 37

Oliguria is usually correlated with what?

Back 37

Acute glomerulonephritis

Front 38

Chylomicrons are primarily composed of what?

Back 38

Triglycerides

Front 39

What is the reaction which differentiates crystals from starch?

Back 39

Starch stains blue black with iodine

Front 40

Identification of an isolate as Yersinia enterocolitica

Back 40

K/A on Kligler's iron agar

Lysine-negative

Front 41

The risk of HbS polymerization is enhanced by:

Back 41

Low (acid) pH

Dehydration

Increased levels of 2,3-DPG

Front 42

Characteristics that help to identify malignant cells:

Back 42

Giant cells

Cellular crowding

High nuclear to cytoplasm (N/C) ratio

Non-uniform nuclei

Irregular nuclear membranes

Multinucleation

Vacuolation

Front 43

Which Lewis blood group system phenotypes usually produces anti-Lea?

Back 43

Le(a-b-)

Front 44

When given during pregnancy, RhIg may...

Back 44

Cross the placenta and sensitize fetal D-positive RBCs.

Front 45

Blastomyces dermatitidis

Back 45

Yeast form is a large, 10-15 um in diameter, thick-walled cell that characteristically produces a single bud attached by a broad base.

Front 46

Paracoccidioides brasiliensis

Back 46

Produces large yeast cells approximately the same size as those of Blastomyces dermatitidis, except multiple buds are attached by narrow necks, giving the appearance of a "mariner's wheel"

Front 47

Sporothrix schenckii

Back 47

Yeast forms are elongated cells that have been called "cigar bodies"

Front 48

Coccidioides immitis

Back 48

Does not produce a yeast form in laboratory culture. It is identified in stained tissue sections by the production of varying sized spherules, ranging from 10 um to over 100 um in diameter. The larger, more mature spherules contain spherical endospores ranging from 2-4 um in diameter.

Front 49

Examples of acute phase proteins

Back 49

Alpha-1 Antitrypsin

C-Reactive Protein

Fibrinogen

Haptoglobin

Front 50

What substance is conjugated with the “indirect” bilirubin to form “direct” bilirubin?

Back 50

Glucuronic acid

Front 51

A phlebotomist at a local hospital recently had an accidental needle stick while drawing blood from a patient being treated for acute hepatitis B. Which serological marker from the patient would be of most value to the physician evaluating the phlebotomist's possible infection status?

Back 51

Hepatitis B e Antigen (HBeAg)

Front 52

Gram positive organisms

Back 52

Resist acetone-alcohol decolorization

Front 53

When performing a transfusion reaction investigation, what is the clerical check used to detect?

Back 53

Errors that may cause an ABO-incompatibility due to patient or donor unit mix up

Front 54

Which assay is performed to diagnose congestive heart failure (CHF)?

Back 54

BNP

Front 55

Principle of competitive radioimmunoassay procedures?

Back 55

Antibody will react equally with labeled and unlabeled antigen

Front 56

The LAP stain

Back 56

Used to determine if an increase of cells is due to chronic myelogenous leukemia or a noncancerous reaction such as an infection. Cells from a noncancerous reaction stain positive with many intense blue granules, while cells from chronic myelogenous leukemia have few blue granules.

Front 57

A patient has a fasting glucose of 130 mg/dl 2-hour & a post prandial glucose of 210 mg/dl...

Back 57

The patient may be diagnosed as having diabetes mellitus

Front 58

What could cause a false positive bilirubin result on a urine chemical reagent strip?

Back 58

Large doses of chloropromazine

Front 59

Howell-Jolly bodies

Back 59

Intra-erythrocyte inclusions represent DNA nuclear fragments which are composed of chromosome-material separated from their mitotic spindles.

Front 60

The reticulocyte count is used to assess what?

Back 60

RBC production by the bone marrow

Front 61

(Urine Creatinine / Serum Creatinine) x Urine Volume (mL) / [ time (hr) x 60 ] =

Back 61

Creatinine Clearance (mL / min)

Front 62

Where should an organizational chart exist?

Back 62

Hospital

Department of Pathology

Each clinical laboratory

Position within a laboratory section

Front 63

What is the purpose of using alcohol in the gram stain procedure?

Back 63

Decolorize all gram negative organisms

Front 64

What is the most suitable specimen for the isolation of Bordetella pertussis?

Back 64

Nasopharyngeal swab

Front 65

Which method remains the “gold standard” for ANA detection?

Back 65

Slide-based immunofluorescent assay (IFA) or Colorzyme®

Front 66

Which blood component would be MOST appropriate for a 9-yr old girl, with a low hemoglobin and low platelet count, who has bleeding gums?

Back 66

Platelet concentrate

Front 67

When AHG or Coombs serum is used to demonstrate that red cells are antibody coated in vivo, the procedure is termed:

Back 67

Direct (DAT) technique

Front 68

The immunoassay procedure for serum hCG utilizes antisera against which subunit of hCG?

Back 68

Beta

Front 69

What is an autoantibody that binds to TSH receptor sites on thyroid cell membranes preventing thyroid-stimulating hormone binding?

Back 69

Thyroid-stimulating immunoglobulin

Front 70

Which phase of the bacterial growth curve demonstrates the greatest growth activity?

Back 70

Log phase

Front 71

Normal distribution curve

Back 71

Data are more likely to fall closer to the mean.

Front 72

Vitamin K-dependent clotting factors

Back 72

II, VII, IX and X

Front 73

Which non-HbS hemoglobins can also sickle and show a positive solubility test?

Back 73

HbC Harlem

Front 74

The nitrite portion of the test strip can be used to:

Back 74

Monitor antibiotic therapy

Detect a bladder infection in an early stage in pregnant women

Screen individuals with recurrent infections

Detect presence of cystitis

Front 75

Granular casts

Back 75

Usually indicative of renal disease

Composed of a protein matrix with degraded cellular material.

Front 76

What MINIMUM level of a particular factor will cause the aPTT test to become prolonged?

Back 76

Less than 40%

Front 77

What are the possible ABO genotypes of offspring of parents whose genotype is AA and BB?

Back 77

AB

Front 78

Siderocytes

Back 78

Red blood cells containing iron granules in their cytoplasm. The granules appear blue/green with Prussian blue stain

Front 79

A patient’s BUN value is 15 mg/dl and his creatinine is 5 mg/dl. If this patient is not undergoing dialysis, what conclusion would you draw from these results?

Back 79

One of the values is in error

Front 80

A 2 month old infant presented with history of chronic diarrhea and failure to thrive. The child's mother also revealed that the baby has suffered from thrush (Candida infections) since birth. Preliminary findings showed lymphopenia (<2,000), hypogammaglobulinemia, and physical findings showed a small thymus, no tonsils or lymph nodes. Which of the following immune deficiencies DOES the patient present?

Back 80

Severe combined immunodeficiency syndrome

Front 81

An increase in CEA levels is most closely associated with which organ?

Back 81

Colon

Front 82

Hapten

Back 82

Incomplete antigen

Front 83

What is the white blood cell that can appear atypical or reactive on a Wright-stained smear?

Back 83

Lymphocyte

Front 84

The heterozygous state of HbS that is referred to as sickle cell trait contains which hemoglobin in combination with S?

Back 84

HbA

Front 85

A CYP2D6 ultrarapid metabolizer (UM) would require ___________ dose of an active drug (non-prodrug) that is metabolized by CYP2D6 than a CYP2D6 extensive metabolizer (EM).

Back 85

A higher

Front 86

Lithium

Back 86

Commonly used in the treatment of manic depressive patients. Lithium exerts a dual effect on receptors for the neurotransmitter glutamate; acting to keep the amount of glutamate active between cells at a stable, healthy level, neither too much nor too little. This allows for a mood stabilization for these patients.

Front 87

Which method is MOST reliable for determining the appropriate dosage of Rh immune globulin to give to an identified Rh immune globulin candidate after delivery?

Back 87

Flow cytometry

Front 88

Polychromatophilic red cells

Back 88

Stain blue/gray with Wright stain and are larger than the normal mature red cells in the field. Another term used to describe these cells is recticulocytes

Front 89

A process by which bacteria or other biological material are preserved through freeze drying under vacuum is termed:

Back 89

Lyophilization

Front 90

What is the primary target of the Hepatitis B virus?

Back 90

Liver

Front 91

Bacterial contamination of a urine specimen from a normal healthy individual could originate from:

Back 91

Contaminated urine container

Vagina

Vulva

Front 92

Examples of media containing ePHI:

Back 92

Electronic medical records on the hospital server hard drive

Recent email containing PHI which is stored on your computer

A CD-ROM disc containing an archive of patient billing information

A nurse's hand held computer containing names of patients with appointment times

Front 93

DONE

Back 93

DONE