This technique answers many questions pertaining to background levels of DNA damage in normal and abnormal cells, variations in repair, regulation of DNA repair, and molecular levels in nucleus. The procedure is fairly simple and can be performed easily by placing a cell on a agrose slide, performing cell lysis, electrophoresis, neutralization, fixation, staining, and microscopy analysis. The comet assay is an important and easy technique, which can be used to determine the about of double stranded and single stranded DNA breaks in a
This technique answers many questions pertaining to background levels of DNA damage in normal and abnormal cells, variations in repair, regulation of DNA repair, and molecular levels in nucleus. The procedure is fairly simple and can be performed easily by placing a cell on a agrose slide, performing cell lysis, electrophoresis, neutralization, fixation, staining, and microscopy analysis. The comet assay is an important and easy technique, which can be used to determine the about of double stranded and single stranded DNA breaks in a