Review of Stacking Interactions of Nucleobases: NMR Investigations by H. Sapper and W. Lohmann
Introduction
Nuclear magnetic resonance (NMR) spectroscopy is often a very useful tool to identify organic molecules based on the atomic nuclei interactions with their environment. Recent advances have made use of the technology in protein, nucleic acid, and complex natural products structure identification. [1, 2, 3] Its origins lie in the works of Felix Bloch and Edward Purcell, who used the discoveries of Isidor Rabi’s extension on Stern-Gerlach experiment, and were awarded the Nobel Prize in 1952 for their use of NMR on solids and liquids. Packard notably observes this phenomena of nuclear resonance when a magnetic field was applied to an organic …show more content…
At the time of the paper’s publication, the CW NMR was not the conventional norm for NMR spectra measurements. Fourier Transfer (FT) NMR was the alternative NMR machine that was available. FT-NMRs offeed better resolution and all nuclei were excited at the same time instead of measuring the absorption of individual nuclei in a sweeping fashion. However, the CW NMR offered an alternative in the way of easy maintenance and low operating costs, but are only used for low resolution 1H NMRs. This requirement was applicable to this experiment, and was understood to be the reason that the CW NMR was used. Currently, the trade-off between resolutions versus operating cost has improved with the development of technology that enable the prevalence of FT-NMR in labs …show more content…
The accuracy of this measured data is important as we are dealing with direct ppm ratios of 6-methylpurine that make a large difference even with tiny changes of the NMR samples or the NMR machine parameters. Comparatively to traditional methods where NMR is used for structural determination, NMR has been used for intermolecular interaction with multiple organic molecules of interest[11] and used today to study the interaction between substrates and enzymatic sites.[12] However, the use of NMR for this particular intermolecular determination is especially unique as it offers a clever way to determine a self-association based on several reasonable assumptions and not simply an intermolecular interaction between two different
Introduction
Nuclear magnetic resonance (NMR) spectroscopy is often a very useful tool to identify organic molecules based on the atomic nuclei interactions with their environment. Recent advances have made use of the technology in protein, nucleic acid, and complex natural products structure identification. [1, 2, 3] Its origins lie in the works of Felix Bloch and Edward Purcell, who used the discoveries of Isidor Rabi’s extension on Stern-Gerlach experiment, and were awarded the Nobel Prize in 1952 for their use of NMR on solids and liquids. Packard notably observes this phenomena of nuclear resonance when a magnetic field was applied to an organic …show more content…
At the time of the paper’s publication, the CW NMR was not the conventional norm for NMR spectra measurements. Fourier Transfer (FT) NMR was the alternative NMR machine that was available. FT-NMRs offeed better resolution and all nuclei were excited at the same time instead of measuring the absorption of individual nuclei in a sweeping fashion. However, the CW NMR offered an alternative in the way of easy maintenance and low operating costs, but are only used for low resolution 1H NMRs. This requirement was applicable to this experiment, and was understood to be the reason that the CW NMR was used. Currently, the trade-off between resolutions versus operating cost has improved with the development of technology that enable the prevalence of FT-NMR in labs …show more content…
The accuracy of this measured data is important as we are dealing with direct ppm ratios of 6-methylpurine that make a large difference even with tiny changes of the NMR samples or the NMR machine parameters. Comparatively to traditional methods where NMR is used for structural determination, NMR has been used for intermolecular interaction with multiple organic molecules of interest[11] and used today to study the interaction between substrates and enzymatic sites.[12] However, the use of NMR for this particular intermolecular determination is especially unique as it offers a clever way to determine a self-association based on several reasonable assumptions and not simply an intermolecular interaction between two different