Physio-Ex Blood Analysis: Computer Simulation Essay

2913 Words May 22nd, 2013 12 Pages
Physio-EX Blood Analysis: Computer Simulation
Introduction
In this lab five blood tests will be performed: hematocrit determination, erythrocyte sedimentation rate, hemoglobin determination, blood typing, and total cholesterol determination. A “hematocrit refers to the percentage of red blood cells (RBC’s) in a sample of whole blood. Since the function of red blood cells is the transport of oxygen to the cells of the body, the higher the hematocrit, the more red blood cells are available to carry oxygen.” In a normal, healthy male the value for males is 47% and in a normal, healthy female it is 42%. A person with a lower than normal hematocrit would indicate they are anemic, and a higher than normal hematocrit would indicate
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Hemoglobin is a protein that is found in red blood cells, this protein is essential for the transportation of oxygen from the lungs to the rest of the cells in the body. “Anemia results when insufficient oxygen is carried in the blood. A quantitative hemoglobin determination is useful for determining the classification and possible causes of anemia and gives useful information on some other disease conditions.” Blood in a healthy individual contains approximately 12-18 grams of hemoglobin per 100 milliliters. “A healthy male has 13.5 to 18 g/100 ml; a healthy female has 12 to 16 g/100 ml.” In a person with polycythemia, congestive heart failure, or someone living in a high altitude location, will have increased hemoglobin. To figure the hemoglobin content, a sample of blood will be stirred with a wooden stick which will rupture, or lyse, the cells. “The intensity of the color of the lysed blood is a result of the amount of hemoglobin present.” To determine the hemoglobin content of a sample, a hemoglobinometer will compare the samples color to standard values. “The hemoglobinometer transmits green light though the hemolyzed blood sample. The amount of light that passes through the sample is compared to standard color intensities.” For this experiment, five samples will be evaluated, a healthy male, healthy female, a female with iron-deficiency anemia, a male with polycythemia, and a female Olympic

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