Lab Report 1: Cell Transport Mechanisms and Permeability Using Physioex 8.0

2035 Words Oct 17th, 2012 9 Pages
Lab Report 1: Cell Transport Mechanisms and Permeability Using PhysioEx 8.0

Introduction The purpose of these experiments is to examine the driving force behind the movement of substances across a selective or semiperpeable plasma membrane. Experiment simulations examine substances that move passively through a semipermeable membrane, and those that require active transport. Those that move passively through the membrane will do so in these simulations by facilitated diffusion and filtration. The plasma membrane’s structure is composed in such a way that it can discriminate as to which substances can pass into the cell. This enables nutrients to enter the cell, while keeping unwanted substances out. Active
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Simulation Runs 2-4 were also done the same way using 50 mmHg of pressure and 5.0 mg/ml of NaCl, urea, glucose, and powdered charcoal dispensed in the top beaker. However, with each run, I changed dialysis membranes. During Run 2, I used the 50 MWCO dialysis membrane, Run 3 used 100 MWCO, and Run 4 used 200 MWCO. After each run the Membrane Residue Analysis Unit was used to detect any residue present on the membrane, and all data was recorded.

Activity 5: Simulating Active Transport In the stimulating active transport experiment, I used the PhysioEx 8.0 Physiology Lab Simulation Program on a computer and the Human Anatomy and Physiology Laboratory Manuel. I used the membrane builder to adjust the sodium-potassium pumps to 500 and the glucose carriers to 500. The membrane was placed between the two beakers. The NaCl concentration in the left beaker was set to 9.00mM and dispensed. KCl concentration in the right beaker was set to 6.00 mM and dispensed. The ATP dispenser on top of the beakers was set to 1.00 MM and dispensed. The timer was set to 60 minutes. I pushed the start button, and watched as solute concentrations of sodium and potassium changed between the two beakers. In Run 2 the same procedures were done again, but this time using an ATP concentration of 3.00 mM. During Run 3, 9.00 mM of NaCl was dispensed in the left beaker and

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