Transgenesis
Transgenesis is the process in which an exogeneous gene (transgene) is added to an organism genome, and this results in expression of the gene which can then be passed down to its offspring. I have chosen to research how the Enviropig has been manipulated using transgenic techniques. The transgene inserted into the Enviropigs genome is Escherichia coli K12’s phytase gene, this gene gives the Enviropigs the ability to produce phytase in their salivary glands, and it gets infused in their saliva. The enzyme of phytase is capable of effectively breaking down the usually indigestible form of phosphorus called phytate in acidic conditions of the stomach, it therefore enables the pigs to digest phytate. Manure of regular pigs ' contains …show more content…
The recognition site is a palindromic sequence; a type of symmetry where the top strand from 3’end to 5’end is the same as the bottom strand from 3’end to 5’end. Each restriction enzyme has different palindromic sequence (recognition site), that it cleaves at. So, a specific restriction enzyme was chosen to make cuts in the mouse PSP promoter sequence, E-coli phytase transgene and the mouse PSP terminator sequence to create sticky ends; makes the ends complementary as they restriction enzyme trims to make them match. This enables the construct to be stuck together by the enzyme DNA ligase. Ligase makes a permanent bond between the segments of the gene construct and also strengthens the sugar phosphate bonding. Only a small number of gene construct is made/attached in the right …show more content…
I have chosen to research how the northern white rhinos may be cloned. The northern white rhinos are a critically endangered species that awaiting extinction; the species only has three lone survivors that are incapable of breeding naturally. Of the three individuals, only one of them is male and this male is 42 years old, so his is in the final few years of his live and has a very low sperm count. Of the two females, one of them has leg injuries therefore this individual isn 't capable of bearing her weight during pregnancy, and the other has a uterus disorder that prevents the individual from mating and from embryo implantation. If nothing is done the northern white rhinos will become extinct in next 40 years. Therefore, the purpose of cloning of the northern white rhinos is to bring this species out of its path to extinction. The process that will most probably be used to achieve this is the somatic cell nuclear transfer, as scientist have frozen tissues from several dead northern white rhinos and the three living rhinos.
The northern white rhinos most probably will be cloned using the somatic cell nuclear transfer (SCNT) method. The following steps will be taken to clone the northern white
Transgenesis is the process in which an exogeneous gene (transgene) is added to an organism genome, and this results in expression of the gene which can then be passed down to its offspring. I have chosen to research how the Enviropig has been manipulated using transgenic techniques. The transgene inserted into the Enviropigs genome is Escherichia coli K12’s phytase gene, this gene gives the Enviropigs the ability to produce phytase in their salivary glands, and it gets infused in their saliva. The enzyme of phytase is capable of effectively breaking down the usually indigestible form of phosphorus called phytate in acidic conditions of the stomach, it therefore enables the pigs to digest phytate. Manure of regular pigs ' contains …show more content…
The recognition site is a palindromic sequence; a type of symmetry where the top strand from 3’end to 5’end is the same as the bottom strand from 3’end to 5’end. Each restriction enzyme has different palindromic sequence (recognition site), that it cleaves at. So, a specific restriction enzyme was chosen to make cuts in the mouse PSP promoter sequence, E-coli phytase transgene and the mouse PSP terminator sequence to create sticky ends; makes the ends complementary as they restriction enzyme trims to make them match. This enables the construct to be stuck together by the enzyme DNA ligase. Ligase makes a permanent bond between the segments of the gene construct and also strengthens the sugar phosphate bonding. Only a small number of gene construct is made/attached in the right …show more content…
I have chosen to research how the northern white rhinos may be cloned. The northern white rhinos are a critically endangered species that awaiting extinction; the species only has three lone survivors that are incapable of breeding naturally. Of the three individuals, only one of them is male and this male is 42 years old, so his is in the final few years of his live and has a very low sperm count. Of the two females, one of them has leg injuries therefore this individual isn 't capable of bearing her weight during pregnancy, and the other has a uterus disorder that prevents the individual from mating and from embryo implantation. If nothing is done the northern white rhinos will become extinct in next 40 years. Therefore, the purpose of cloning of the northern white rhinos is to bring this species out of its path to extinction. The process that will most probably be used to achieve this is the somatic cell nuclear transfer, as scientist have frozen tissues from several dead northern white rhinos and the three living rhinos.
The northern white rhinos most probably will be cloned using the somatic cell nuclear transfer (SCNT) method. The following steps will be taken to clone the northern white