Essay on Evaluating The Function Of Genes

862 Words Dec 6th, 2016 4 Pages
Genome targeting and editing has provided the ability to better understand and determine the function of genes in many model organisms. Genomes are composed of genes which are the basic unit of heredity that determine the traits organisms express. With the ability to target specific genes and edit them, genes and their locations in the genome can be studied and determine their significance and function, in addition to gaining an understanding to human disease and possible routes to eliminate them. The most effective methods of genome editing are those of the zinc-finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and the clustered regulatory interspaced short palindromic repeats/CRISPR-associated systems (CRISPR/Cas).
Prior to the discovery and the development of the ZFN, TALE and CRISPR/Cas methods, genome targeting and editing seemed to have more negative effects than positive due to the amount of time and effort it required and the high rate of error. The method employed prior to the ZFN, TALE and CRISPR systems was a targeting construct paired with homologous recombination which was primarily limited to the alteration of embryonic stem cells due to the pluripotency of the cells.
Zinc-finger proteins are artificially synthesized proteins created to edit DNA strands at specific sites which allowed for the process to become one of the most types of DNA bonding due to the high rate of specificity. The fact that it is created from arrays that have…

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