Parasitology Lab Practical Hemoflagellates

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cell types in order trypanosomatida
1. flagellum, anterior
2. nucleus
3. kinetoplast, posterior
A. amastigote
B. Promastigote
C. epimastigote
D. trypomastigote

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what type of reproduction is this?

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Leishmania, asexual reproduction
1. amastigote
2. macrophage
3. promastigote

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Leishmania, promastigotes
1. nucleus
2. flagellum
3. kinetoplast

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Leishmania, promastigotes

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Cutaneous leishmaniasis, film from edge of ulcer
1. macrophage nucleus
2. amastigote

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Cutaneous leishmaniasis

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Cutaneous leishmaniasis, healed lesion

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Cutaneous leishmaniasis, ear lesion with erosion

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Cutaneous leishmaniasis, shin lesion

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Muco-cutaneous Leishmaniasis 1yr

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Muco-cutaneous Leishmaniasis 3yr

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Muco-cutaneous Leishmaniasis parrot beak

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Hepatosplenomegaly in visceral leishmaniasis

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Amastigotes of L. donovani in spleen
1. amastigote
2. macrophage nucleus

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Leishmania donovani, promastigotes in culture

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Trypanosoma cruzi Romana sign, periorbital edemal related to chagoma

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Trypanosoma cruzi life cycle
1. metacyclic trypomastigote
2. amastigote intracellular
3. trypomastigote extracellular
4. epimastigote

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Trypanosoma cruzi, epimastigotes from tissue culture
1. nucleus
2. kinetoplast
3. flagellum

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Trypanosoma cruzi, trypomastigote in blood film
1. nucleus and undulating membrane
2. kinetoplast

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Trypanosoma cruzi, trypomastigote in blood film during acute infection(right)
T. rangeli, non-pathogenic TM in wildlife and humans in S. america (left)

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Trypanosoma cruzi, amastigotes in cardiac muscle
1. amastigotes
2. cardiac muscle nuclei

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Trypanosoma cruzi, myocardial pathology

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Trypanosoma cruzi, chronic infection with megacolon

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Trypanosoma brucei gambiense life cycle
1. metacyclic trypomastigote
2. extracellular replication at bite site
3. trypomastigote
4. trypomastigotes replicate in fly gut, then migrate to salivary glands and transform into epimastigotes

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Trypanosoma brucei, trypomastigotes in blood film.
1. small kinetoplast
2. binary fission in progress

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Trypanosoma brucei, blood film. Note minute kinetoplast and rounded posterior of the TM

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Trypanosoma lewisi, non-pathogenic TM in rats. Useful model for immunologic research. Natural transmission by fleas but transmitted in the laboratory by blood inoculation.