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128 Cards in this Set
- Front
- Back
large nutrients. Major role in cell structure and metabolism (carbohydrates, fats, protein) |
Macronutrients |
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"Trace elements" smaller. Enzyme function and protein structure (zinc, iron, nickel) |
Micronutrients |
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Must obtain carbon as source in order to be absorbed by the cell |
Heterotroph |
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Makes own nutrients from carbon dioxide through photosynthesis |
Autotroph |
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Use photosynthesis to feed. Sunlight is the energy source |
Phototroph |
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Obtain energy from chemical compounds |
chemotrophs |
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Energy comes from sunlight |
Photoautotroph |
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Breaks down chemical compounds |
Chemoautotroph |
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Produce methane (archea) Hydrogen gas and carbon dioxide. aerobic |
Methanogens |
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Rely on inorganic compounds |
Lithoautotrophs |
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use organic compounds for energy and inorganic compounds as carbon source |
Chemoorganic Autotrophs |
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Feeds on waste from dead oragnisms |
Saprobe |
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"Free Living" Gets nutrients from living host cell |
Parasite |
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"Solvent" From low to high concentration. Movement of water across membrane |
Osmosis |
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"Solute" form high to low concentration. |
Diffusion |
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Molecules that move in random motion |
Simple Diffusion (Passive Transport) |
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Molecules bind to a specific receptor in membrane and carried to the other side |
Facilitated Difussion(Passive Transport) |
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Molecules are pumped in and out of cell |
Carrier Mediated (Active Transport) |
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Molecules move across membrane and converted into a useful substance |
Group Translocation (Active Transport) |
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Measures the amount of microbial growth |
Turbidometry |
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Counts the number of cells |
Direct (total) Cell |
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Measures the size of the cell |
Flow Cytometer |
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All chemical reactions within a cell. small to large macromolecules to utilize energy. Large to small macromolecules to yield ATP |
Metabolism |
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Build macromolecules |
Anabolism |
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Break down macromolecules to release energy |
Catabolism |
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Speeds up the reaction of metabolism |
Enzyme |
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Speeds up the rate of chemical reaction (example: zinc) |
Catalyst |
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Constant amounts |
Constitutive Enzymes |
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Enzymes that can be induced or repressed |
Regulated Enzymes |
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Allostearic site. Does not compete with substrate |
Noncompetitive Inhibition |
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RNA molecules that catalyze reactions on other RNA molecules |
Ribozyme |
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Pure Protein |
Simple Enzyme |
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Protein portion of enzyme |
Apoenzyme |
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Non protein. helps substrate bind to active site |
Cofactor |
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Organic cofactors |
Coenzyme |
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Active enzyme |
Apoenzyme and cofactor |
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Haloenzyme |
Apoenzyme and coenzyme |
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Conjugated enzyme |
Haloenzyme |
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Most common electon carrier |
NAD |
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Oxygen is final electron acceptor |
Aerobic metabolism |
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Inorganic or organic compounds |
Anaerobic metabolism |
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Compound that is a source of another compound. (Example: Acetyl CoA) |
Precursor Molecule |
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RNA, DNA, Pyruvate, Acetyl CoA |
Cellular Building Blocks |
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Series of redox reactions growing during final phase |
Oxidative Phosphorylation |
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Adding inorganic phosphate. Stores ATP. Sunlight driven |
Phosphorylation |
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ATP formed by a transfer of phosphate group |
Substrate Level Phosphorylation |
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Reduce pyruvic acid to lactic acid |
Homolactic |
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Glucose ferment to mixture of lactic acid |
Hetrolatic |
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Gaseous Oxygen |
Aerobe |
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Cannot live without oxygen |
Obligate Aerobe |
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Can grow with or without oxygen |
Facultative Anaerobe |
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Will die if exposed to oxygen |
Anaerobe |
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Uses a small amount of oxygen |
Microaerophile |
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Oxygen doesnt harm growth |
Aerotolerant |
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Utilizes oxygen containing ions |
Anaerobic respiration |
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Free oxygen as final electron acceptor |
Aerobic respiration |
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Grows best at high CO2 |
Capnophile |
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Lives in High sugar environment |
Osmophile |
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Lives in high level salt environment |
Halophile |
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Grows best at high level salt environments |
Facultative Halophile |
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Serve multiple functions |
Amphibolism |
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Converts carbon dioxide into organic compounds using energy from the sun |
Photosynthesis |
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Use energy to synthesize glucose |
Calvin Cycle |
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Sum total of genetic material |
Genome |
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Unit of heredity from parent to offspring |
Gene |
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Genetic Makeup |
Genotype |
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Expresses the genotype that create structures or functions |
Phenotype |
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Basic unit of DNA. Phosphate, deoxyribose sugar, nitrogenous gas |
Nucleotide |
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Factor of DNA synthesis and production |
Leading and lagging strands |
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Incomplete. Said that mutations come from RNA segments instead of genes for proteins |
Central Dogma |
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mRNA, tRNA, rRNA are involved in |
Translation |
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Codes for one protein |
Eukaryotic Transcription |
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Contains information from several genes |
Bacterial Transcription |
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Study of organisms genes and functions |
Genomics |
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Study of proteins and functions |
Proteomics |
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Set of genes that are repressed or induced |
Operons |
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Allostearic absence of lactose repressor, binds to block transcription |
Repressor Protein |
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Any organism that expresses and contains genes that orignated in another organism |
Recombinant |
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Genetic transfer from donor to recipient (Direct) |
Conjugation |
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Acceptance from a bacterial cell of small fragments of DNA from surrounding environment |
Transformation |
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Bacteriophage serve as carrier of DNA from donor to recipient. Must be same species |
Transduction |
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1920 Freddrick Griffith studied streptococcus pnemoniae |
Transformation |
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Any change in a nucleotide sequence |
Mutation |
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When bases are added or deleted |
Frame shift |
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Fixes ultraviolet damage in Frame shift |
Photoreactivation |
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Remove incorrect bases |
Excision |
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Changes normal codon to stop codon |
Nonsense Mutation |
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Alters bases but has no effect |
Silent Mutation |
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Any change to code that replace a different amino acid |
Missense Mutation |
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Free of spores |
Sterile |
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Kills microbes but not spores or prions |
Disinfection |
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Removing micorbes on the body (degermination) |
Antisepsis |
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Lowers micorbial numbers (Sanitization) |
Decontamination |
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Means to kill |
Cidial |
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Means to stop growth |
Static |
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Temps range from 60 to 135 degrees Celsius |
Moist Heat method |
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Temps range from 160 degrees Celsius and higher |
Dry Heat method |
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Shortest length to kill all test microbes at a certain temp |
Thermal Death Time |
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Lowest temp to kill all microbes in a sample in 10 min |
Thermal Death Point |
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Boiling water , pasteurization, nonpressurized steam |
Moist Heat |
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Incinerator, hot air oven, Bunsen Burner |
Dry heat
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Kills some microbes , but some still leak toxic products |
Cold Treatment |
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Removing moisture from foods, but some microbes can withstand and become dormant |
Desiccation |
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Highly effective control method |
Ionizing radiation |
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Not as penetrating |
Nonionizing radiation |
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Removes microbes from air and fluids, alternative for microbes who die in heat |
Filtration |
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Adding high volumes of sugar or salt to create a hypertonic environment |
Osmotic Pressure |
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Florine, Chlorine, bromine, iodine |
Halogen agents |
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Destroy vegatative microbial forms. Too toxic for antisepsis. Ethyl and isopropyl. 70% is more efficient . More effective with enveloped viruses |
Alcohol |
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Cleanse wounds. Acts as anitspetic. Helps disinfect surgical equipment |
Hyrdogen Peroxide |
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Forms water soluble interface. limited microcidal power |
Surfactant |
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Identifies if bacteria is sensitive to a drug |
Kirby Bauer Test |
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Examination for growth |
Tube dilution test |
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Ratio of drugs that are toxic to human |
Therapeutic Index |
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Drug that kills microbes without harming human host |
Penicillin |
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Destroys beta lactam ring. |
Beta lactamases |
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Similar to penicillin |
Cephalosporins (Cephalosporium acremonium) |
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Target DNA and RNA. Stops DNA transcription |
Quinolones |
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Bacteria that is resistant to a antibiotic that they produce themselves |
Intrinsic |
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Bacterial resistance to a drug that they were previously sensitive to |
Acquired |
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Improve intestinal biota |
Probiotics |
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Encourage growth of beneficial microbial growth in intestines |
Prebiotics |
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Penicillin, Vancomycin, isoniazed, cephalosporin |
Cell Wall Inhibitor |
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narrow spectrum drug, target mycolic wax, mycobacterium |
Isoniazed |
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broad spectrum drug, few complications |
Cephalosporin |
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Erythromycin, Streptomycin, tetracycline (70s ribosomes) |
Protein Synthesizers |
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Sulfonamides, P-amino benzoic acid (PABA) |
Folic Acid Synthesizers |
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Drug that creates nucleotides |
P-amino benzoic acid (PABA) |