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128 Cards in this Set

  • Front
  • Back

large nutrients. Major role in cell structure and metabolism (carbohydrates, fats, protein)

Macronutrients

"Trace elements" smaller. Enzyme function and protein structure (zinc, iron, nickel)

Micronutrients

Must obtain carbon as source in order to be absorbed by the cell

Heterotroph

Makes own nutrients from carbon dioxide through photosynthesis

Autotroph

Use photosynthesis to feed. Sunlight is the energy source

Phototroph

Obtain energy from chemical compounds

chemotrophs

Energy comes from sunlight

Photoautotroph

Breaks down chemical compounds

Chemoautotroph

Produce methane (archea) Hydrogen gas and carbon dioxide. aerobic

Methanogens

Rely on inorganic compounds

Lithoautotrophs

use organic compounds for energy and inorganic compounds as carbon source

Chemoorganic Autotrophs

Feeds on waste from dead oragnisms

Saprobe

"Free Living" Gets nutrients from living host cell

Parasite

"Solvent" From low to high concentration. Movement of water across membrane

Osmosis

"Solute" form high to low concentration.

Diffusion

Molecules that move in random motion

Simple Diffusion (Passive Transport)

Molecules bind to a specific receptor in membrane and carried to the other side

Facilitated Difussion(Passive Transport)

Molecules are pumped in and out of cell

Carrier Mediated (Active Transport)

Molecules move across membrane and converted into a useful substance

Group Translocation (Active Transport)

Measures the amount of microbial growth

Turbidometry

Counts the number of cells

Direct (total) Cell

Measures the size of the cell

Flow Cytometer

All chemical reactions within a cell. small to large macromolecules to utilize energy. Large to small macromolecules to yield ATP

Metabolism

Build macromolecules

Anabolism

Break down macromolecules to release energy

Catabolism

Speeds up the reaction of metabolism

Enzyme

Speeds up the rate of chemical reaction (example: zinc)

Catalyst

Constant amounts

Constitutive Enzymes

Enzymes that can be induced or repressed

Regulated Enzymes

Allostearic site. Does not compete with substrate

Noncompetitive Inhibition

RNA molecules that catalyze reactions on other RNA molecules

Ribozyme

Pure Protein

Simple Enzyme

Protein portion of enzyme

Apoenzyme

Non protein. helps substrate bind to active site

Cofactor

Organic cofactors

Coenzyme

Active enzyme

Apoenzyme and cofactor

Haloenzyme

Apoenzyme and coenzyme

Conjugated enzyme

Haloenzyme

Most common electon carrier

NAD

Oxygen is final electron acceptor

Aerobic metabolism

Inorganic or organic compounds

Anaerobic metabolism

Compound that is a source of another compound. (Example: Acetyl CoA)

Precursor Molecule

RNA, DNA, Pyruvate, Acetyl CoA

Cellular Building Blocks

Series of redox reactions growing during final phase

Oxidative Phosphorylation

Adding inorganic phosphate. Stores ATP. Sunlight driven

Phosphorylation

ATP formed by a transfer of phosphate group

Substrate Level Phosphorylation

Reduce pyruvic acid to lactic acid

Homolactic

Glucose ferment to mixture of lactic acid

Hetrolatic

Gaseous Oxygen

Aerobe

Cannot live without oxygen

Obligate Aerobe

Can grow with or without oxygen

Facultative Anaerobe

Will die if exposed to oxygen

Anaerobe

Uses a small amount of oxygen

Microaerophile

Oxygen doesnt harm growth

Aerotolerant

Utilizes oxygen containing ions

Anaerobic respiration

Free oxygen as final electron acceptor

Aerobic respiration

Grows best at high CO2

Capnophile

Lives in High sugar environment

Osmophile

Lives in high level salt environment

Halophile

Grows best at high level salt environments

Facultative Halophile

Serve multiple functions

Amphibolism

Converts carbon dioxide into organic compounds using energy from the sun

Photosynthesis

Use energy to synthesize glucose

Calvin Cycle

Sum total of genetic material

Genome

Unit of heredity from parent to offspring

Gene

Genetic Makeup

Genotype

Expresses the genotype that create structures or functions

Phenotype

Basic unit of DNA. Phosphate, deoxyribose sugar, nitrogenous gas

Nucleotide

Factor of DNA synthesis and production

Leading and lagging strands

Incomplete. Said that mutations come from RNA segments instead of genes for proteins

Central Dogma

mRNA, tRNA, rRNA are involved in

Translation

Codes for one protein

Eukaryotic Transcription

Contains information from several genes

Bacterial Transcription

Study of organisms genes and functions

Genomics

Study of proteins and functions

Proteomics

Set of genes that are repressed or induced

Operons

Allostearic absence of lactose repressor, binds to block transcription

Repressor Protein

Any organism that expresses and contains genes that orignated in another organism

Recombinant

Genetic transfer from donor to recipient (Direct)

Conjugation

Acceptance from a bacterial cell of small fragments of DNA from surrounding environment

Transformation

Bacteriophage serve as carrier of DNA from donor to recipient. Must be same species

Transduction

1920 Freddrick Griffith studied streptococcus pnemoniae

Transformation

Any change in a nucleotide sequence

Mutation

When bases are added or deleted

Frame shift

Fixes ultraviolet damage in Frame shift

Photoreactivation

Remove incorrect bases

Excision

Changes normal codon to stop codon

Nonsense Mutation

Alters bases but has no effect

Silent Mutation

Any change to code that replace a different amino acid

Missense Mutation

Free of spores

Sterile

Kills microbes but not spores or prions

Disinfection

Removing micorbes on the body (degermination)

Antisepsis

Lowers micorbial numbers (Sanitization)

Decontamination

Means to kill

Cidial

Means to stop growth

Static

Temps range from 60 to 135 degrees Celsius

Moist Heat method

Temps range from 160 degrees Celsius and higher

Dry Heat method

Shortest length to kill all test microbes at a certain temp

Thermal Death Time

Lowest temp to kill all microbes in a sample in 10 min

Thermal Death Point

Boiling water , pasteurization, nonpressurized steam

Moist Heat

Incinerator, hot air oven, Bunsen Burner

Dry heat


Kills some microbes , but some still leak toxic products

Cold Treatment

Removing moisture from foods, but some microbes can withstand and become dormant

Desiccation

Highly effective control method

Ionizing radiation

Not as penetrating

Nonionizing radiation

Removes microbes from air and fluids, alternative for microbes who die in heat

Filtration

Adding high volumes of sugar or salt to create a hypertonic environment

Osmotic Pressure

Florine, Chlorine, bromine, iodine

Halogen agents

Destroy vegatative microbial forms. Too toxic for antisepsis. Ethyl and isopropyl. 70% is more efficient . More effective with enveloped viruses

Alcohol

Cleanse wounds. Acts as anitspetic. Helps disinfect surgical equipment

Hyrdogen Peroxide

Forms water soluble interface. limited microcidal power

Surfactant

Identifies if bacteria is sensitive to a drug

Kirby Bauer Test

Examination for growth

Tube dilution test

Ratio of drugs that are toxic to human

Therapeutic Index

Drug that kills microbes without harming human host

Penicillin

Destroys beta lactam ring.

Beta lactamases

Similar to penicillin

Cephalosporins (Cephalosporium acremonium)

Target DNA and RNA. Stops DNA transcription

Quinolones

Bacteria that is resistant to a antibiotic that they produce themselves

Intrinsic

Bacterial resistance to a drug that they were previously sensitive to

Acquired

Improve intestinal biota

Probiotics

Encourage growth of beneficial microbial growth in intestines

Prebiotics

Penicillin, Vancomycin, isoniazed, cephalosporin

Cell Wall Inhibitor

narrow spectrum drug, target mycolic wax, mycobacterium

Isoniazed

broad spectrum drug, few complications

Cephalosporin

Erythromycin, Streptomycin, tetracycline (70s ribosomes)

Protein Synthesizers

Sulfonamides, P-amino benzoic acid (PABA)

Folic Acid Synthesizers

Drug that creates nucleotides

P-amino benzoic acid (PABA)