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11 Cards in this Set
- Front
- Back
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How do you calculate the total magnification of a specimen
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Objective lens X Ocular lens = total magnification
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How much is the magnification of the ocular lens?
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10
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Darkfield Microscopy
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Unstained Specimens in liquid
Examinations of the internal structures of living microbes |
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Differential Interference Contrast Microscopy (DIC)
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Examination living microbes, the image is colored and appears in 3D
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Transmission electron Microscopy TEM
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Good for observing thin sections of organisms and tissues.
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Scanning electron microscopy
SEM |
For viewing Surface structures of microbes in 3D
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What is the Mordant
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Iodine
It improves bonding between the stain and the specimen. |
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Gram Staining
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Most important staining techniques in medical microbiology
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What can you find out from Gram Staining
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Whether it is Gram + or -
The differential staining of the two types of bacteria is a reflection for the differences in the basic composition of their cell wall and their ability to retain Crystal violet-iodine. |
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Gram Positive Bacteria
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Thick Peptidoglycan layer in cell wall and no lipopolysaccharide LPS
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Gram Negative bacteria
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Thin peptidoglycan layer
Lipopolysaccharide Cell wall: Out membrane, periplasm, inner membrane |
