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34 Cards in this Set

  • Front
  • Back

Catabolism

- Breaks down complex molecules; provides energy and building blocks for anabolism


- Exergonic


Ex: Glucose to CO2 and H2O

Anabolism

- Uses energy and building blocks to build complex molecules


- Endergonic


Ex: Amino acids to Proteins

Enzymes

- Act as biological catalysts

Active Site

- Area where the enzymes associates directly with the substrate

Induced Fit

- Moment of conformational change when substrates binding to the active site causes the enzyme-substrate complex to undergo a slight change in shape to accomodate the substrate

Energy of Activation

- Energy and/or biological catalysts allow reacts that normally don't place to activate by lowering its ___________________________________.

Cofactors

- Nonprotein (non-organic) activator


- Almost always vitamins

Coenzymes

- Protein, Organic activators for enzymes

Temperature


pH


Substrate Concentration


Inhibitors

4 Things that Influence Enzyme Activity

Chemotherapy


Antibiotics

2 Medical Examples of Inhibitors

Oxidation




Reduction

- Removal of electrons




- Gain of electrons

Redox Reaction

- Oxidation reaction paired with a reduction reaction

Chemiosmosis

- Electrons (from NADH) pass down the electron transport chain while protons H+ are pumped across the membrane


- Established proton H+ gradient (for motive force) so they can diffuse through ATP synthase and release energy to synthesize ATP

Plasma Membrane




Mitochondria Outer membrane

Where is the electron transport chain in bacteria?




In eukarya?

Fermentation

- Releases energy from the oxidation of organic molecules


- Does not require O2, or use Krebs Cycle or ETC


- Uses organic molecule as final electron acceptor instead of O2


- Produces small amounts of ATP




- Depending on what they produce, helps identify bacteria

Lactic Acid Fermentation - Lactic Acid




Alcohol Fermentation - Ethanol + CO2

Name 2 Types of Fermentation and what they produce

Acetic Acid Bacteria

- Bacteria that require oxygen to oxidise alcohol to its end product




Ex: Clostridium, enterobacter, escherichia, Salmonella

Fermentation Test

- Bacteria that catabolize carbohydrate or protein produce acid, causing the pH indicator to change color


- If gas is present, bubble will appear in the inverted tube




What does this describe?

Biochemical Test

- How to identify bacteria by detecting enzymes (e.g., those involved in decarboxylation and dehydrogenation)


- Such as the amino acid catabolizing enzyme

Peptone iron agar turns


Black


In the presence of


H2S

Example of Biochemical Test

Carbon Source


Mechanisms of Carbohydrate catabolism


ATP generation

3 Ways to sort Metabolic Diversity among Organisms

Photoautotrophs




- Oxygenic: Produces O2


- Anoxygenic: Does not produce O2

- Organisms that use energy in the Calvin-Benson cycle to fix atmospheric CO2 to sugar


- Similar to plants




- Give 2 classifications



Photoheterotrophs

- Organisms that uses energy from light and organic compounds as sources of carbon


- Anoxygenic

Chemoautotrophs

- Organisms that use energy from inorganic chemicals


- CO2 is its carbon source


- Energy is used in the Calvin-Benson cycle to fix CO2

Chemoheterotrophs

- Organisms that use energy and carbon from organic chemicals


- Medically and economically important


- Ex: Humans

Photoautotrophs


Photoheterotrophs




Chemoautotroph




Chemoheterotroph

- What nutritional types uses light as its energy source?




- What nutritional type uses inorganic chemicals as its energy source?




- What nutritional type uses chemicals as its energy source?

Photoautotroph


Chemoautotroph




Photoheterotroph


Chemoheterotroph

- Nutritional types that use CO2 as its carbon source




- Nutritional types that use organic compounds as its carbon source

- Phototroph


- Carbon Source: CO2


- Uses H2O to reduce CO2

Explain the nutritional classification of oxygenic photosynthetic organisms




(Chemo/Phototroph?, Carbon Source? Does it reduce CO2 with water?)

- Phototroph

- Carbon Source: CO2


- Does not use H2O to reduce CO2

Explain the nutritional classification of anoxygenic photosynthetic organisms




(Chemo/Phototroph?, Carbon Source? Does it reduce CO2 with water?)

Phototroph


Carbon Source: Organic Compounds


Photoheterotroph

Explain nutritional classification of green and purple nonsulfur bacteria




(Energy Source? Carbon Source?)

Chemotroph


Carbon Source: CO2


Chemoautotroph

Explain nutritional classification of hydrogen-, sulfur-, iron-, nitrogen-, and carbon monoxide- oxidizing bacteria




(Energy Source? Carbon Source?)

Chemotroph


Carbon Source: CO2


Final Electron Acceptor: Inorganic


Electron Transport Chain

Explain nutritional classification of Clostridium.




Energy Source? Carbon Source? Final Electron Acceptor? If not O2 than organic or inorganic compound? How does it metabolize?

Chemotroph


Carbon Source: CO2


Final Electron Acceptor: Organic


Fermentation

Explain nutritional classification of Streptococcus.




Energy Source? Carbon Source? Final Electron Acceptor? If not O2 than organic or inorganic compound? How does it metabolize?

Chemotroph


Carbon Source: Organic Compound


Final electron Acceptor: O2

Explain nutritional classification of animals, most fungi, protozoa, bacteria.




Energy Source? Carbon Source? Final Electron Acceptor? If not O2 than organic or inorganic compound? How does it metabolize?