Micro Lab 2 Exam

Front 1

what test is this?

Back 1

D Acid reacts wit methyl red ph indicator to go from yellow to a red color (positive),while it remains yellow (negative).MR uses d mixed acid pathway

Front 2

identify this test?

Back 2

VP (Voges-Proskauer)test.The reagents used were alpha-naphthol & KOH.The reagents react wit acetoine & butanediol to give a crimson red color(pos),while neg turns copper color.VP uses 2,3-butanediol pathway

Front 3

Which bacteria uses d mixed acid pathway,fermentation pathway(MR)?

Back 3

E.coli & related bacteria.This pathway produces alot of organic acids including lactic acid,acetic acid,formic acid,succinic acids.

Front 4

Which bacteria uses d 2,3-butanediol pathway,fermentation pathway(VP)?

Back 4

Enterobacter aerogenes & related bacteria

Front 5

identify this test

Back 5

Phenylalanine deaminase test.Ferric Chloride(FeCl3) reagent is added to d surface of d medium. phenylpyruvic acid reacts wit ferric ions & turns green at d surface of medium (pos+)Negative result remains clear

Hint 5

deaminase means to remove an amine group from d amino acid phenylalanine to produce ammonia & phenylpyruvic acid,which can b used as a carbon & energy source.

Front 6

what's this pathogen ?

Back 6

Neisseria gonorrhoeae causes gonorrhea.Etiologic agent:Gram neg diplococcusbacterium.MOT:sex,mom➡baby.Sx: painful urinatn,pus disch arge,sterility,ectopic pregnancy

Front 7

what's d etiological agent for Neisseria gonorrhoeae?

Back 7

Gram negative diplococcus bacterium

Front 8

what's this pathogen?

Back 8

taenia solium causes taeniasis or cysticercosis.ethiological agent is cestode (tapeworm)MOT is by eating undercooked pork & fecal-oral

Hint 8

tape worm (eating undercooked pork

Front 9

what's d etiological agent of taenia solium?

Back 9

cestode (tapeworm )transmitted via eating undercooked pork(taeniasis),
fecal-oral(cysticercosis)

Front 10

what pathogen is this?

Back 10

trypanosoma Bruce gambiense causes African trypanosomiasis/sleeping sickness.MOT: (vector)Tse Tse fly.ethiologic agent is protozoa Sx:swollen lymph,confusion(reaches d brain)

Hint 10

swimming stuff

Front 11

identify d pathogen

Back 11

Ascaris lumbricoides causes Ascariasis.Etiologic agent:Nematode (roundworm)MOT:fecaloral.Sx:wheezing,SOB,persistent cough

Hint 11

sympin lungs includes vomiting, persistentcoughing, wheezing
,shortness of breathe . mammillated (nipples),so non mallilated(no nipple)

Front 12

identify d pathogen?

Back 12

Epstein-Barr virus(aka kissing disease) causes infectious mononucleosis.Etiologic agent is a virus.MOT is saliva.Sx:swollen glands in d neck,sore throat,sore muscles

Front 13

identify d pathogen?

Back 13

Bacillus anthracis causes Inhalation al anthrax.Etiologic agent is Gram positive rod.Sx:coughing up blood, shortness of breathe,shock,meningiti

Hint 13

affects lung tissues

Front 14

identify d pathogen?

Back 14

plasmodium falciparum causes cerebral malaria/severe malaria Etiologic agent is protozoa.MOT:Anop
heles mosquito(vector)Sx:neurologica
symptoms,state of consciousness altered

Front 15

identify d pathogen?

Back 15

Schistosomiasis mansoni causes Schistosomiasis.Etiologic agent is Trematodes eggs(fluke).MOT:larvae burrow thru skin.Sx:blood in urine/stool,seizures,cancer of d liver,

Hint 15

look for horns,also a flat worm also causes katayama fever

Front 16

identify d pathogen?

Back 16

Candida albicans causes Candidiasis. Etiologic agent is yeast.MOT: overgrowth of one's own normal flora due to antibiotics.Sx:jointpain

Hint 16

look for budding yeast

Front 17

identify d pathogen?

Back 17

Giardia lamblia causes Giardiasis Etiologic agent is protozoa.MOT: fecal-oral,thru H2O.Sx:flatulence,gre
asy stool,diarrhea,dehydration

Hint 17

looks like tear drop causes gas

Front 18

what lab is dis?

Back 18

oral microbiota lab showing diversity of bacteria in our mouth(gram-&+rods & cocci)

Front 19

what lab is dis?

Back 19

Staphlococcus aureus Agglutination test using blue liquid wit latex beads covered in anti-staph Ab.add unkn Ag:dark beads clumping=pos (health
y carrier ).if no clumping,fluid smear=Neg

Hint 19

Rapid test(passive agglutination test)Direct test looking for Ag.Staph areas test taking samples from cultured large beta hemolytic Colonie
A pos resul+Sx & symp confirm diagnosis for staph aureus infection

Front 20

Rapid tests are

Back 20

immunological assays that use Ag-Ab reactions to identify a specific agent or organism within mins,maybe at a docs office or hospital labs (E.g Staph aureus & streptococcus pyogenes test.

Front 21

identify this test?

Back 21

Amylase test for starch.pos:clearing, bcos starch has been used up. Neg:brown color right up d growth line cos d reagent iodine, reacted wit starch left over .starch agar is d medium used.

Hint 21

think abt metabolic pathways:exoenzymes

Front 22

identify this test?

Back 22

Lipase test tests 4 lipase enzyme,uses trybutyrin(tryglyceride)agar.Pos=clearing cos lipase is used up.Neg=cloudy right up to d growth line

Front 23

identify this test?

Back 23

Gelatinase test.tests 4 d enzyme gelatin(protein derv.from collagen) Gelatin agar plate incubated at 22'C Pos=clearing cos it's been used.Neg
=cloudy up to growth line

Hint 23

Above 25'C,it bcoms liquid & below 25'C will be solid,hence we incubate at 22'C

Front 24

identify this test?

Back 24

Nitrate reduction test.Nitrate broth. N2(denitrification)➡ NO2(nitrate reductase)➡NO3.Pos:N2=bubbles.if no bubbles,test for NO2 prod. use reagents TDR & sulfuric acid,Pos=

Hint 24

purple-brown,neg= yellow.Then NO3 test's done using reagent:Zinc,which reacts with any nitrate to give purple-brown color,neg=yellow meaning it's been reducd 2 anoda prod.like urea.

Front 25

what's a positive result & y?

Back 25

pos= bubbles bcos d enzyme catalase dat breaks down hydrogen peroxide(substrate)to H2O & O2.Neg=no bubbles

Hint 25

Catalase Test tests 4 d enzyme catalase Note,aerobic & facultative organisms ve catalase enzymes dat helps dem turn H2O2 to harmless H2O & O2,however,obligate anaerobe lack catalase,so they can't tolerate living in air

Front 26

what does d oxidase test tests for?

Back 26

cytochrome c oxidase.uses BBL dry slide wit reagent.pos=purple-brown color in 20sec cos cytochrome c oxidase oxidizes cytochrome if present.neg=no color change or light gray

Hint 26

test cannot be read after 20secs cos a negative result can change to purple after d 20sec

Front 27

is this test testing for fermentation of glucose(GLS)Yes

Back 27

starts as green medium,then it be omes yellow after adding bacteria(+ acid A),AG=pos,cos d bacteria ve d enzyme to use d particular sugar. blue=alkaline(using protein instead)

Hint 27

NC=no change . Catabolism of carbohydrates lab

Front 28

what test is this?

Back 28

methyl red(MR)reagent:methyl red indicator.D acid react wit d MR reagent to produce a red color cos ph indicator dat turns red,forming an acid➡MR-VP broth's used.Neg =Yellow.methyl red indicator

Hint 28

Tests for glucose fermentation pathway.MR is mixed acid pathway(E.coli group)

Front 29

identify this test

Back 29

VP(Voges-Proskauer test)tests for 2,3 butanediol pathway producing acetoine.Pos=crimson red bcos of d reactn wit acetoine.Neg=Cu color due to reactn of reactant wit each other.
uses alpha naphtol & KOH reagents

Hint 29

I'd say dark red to me.MR-VB broth Enterobacter aerogenes group uses dis pathway

Front 30

identify this test

Back 30

H2S test for d enzymes cysteine disulfurase using SIM medium,pos= black precipitate (ferrous sulfide)due to H2S reactn with iron salts in d medium.Neg=remains yellow

Hint 30

H2S means hydrogen sulfide(catabolism of protein )

Front 31

identify this test

Back 31

Indole test tests for d enzyme tryptophanase.SIM medium.paper strip containing oxalic acid strip (reagent) reacts wit indole to give a pink color(pos)Neg:strip remains white

Hint 31

catabolism of protein test

Front 32

identify d test

Back 32

motility test.to assess stabbing line for motility(3 D,fuzzy appearance around stab line) in tubes dat remained yellow after H2S testing.

Hint 32

H2S tube that tested black is already positive for motility.wen assessing stab lines in yellow tubes,twisted or straight ribbon re negative

Front 33

identify this test

Back 33

phenylalanine deaminase test tests for phenylalanine deaminase using phenylalanine agar.Ferric chloride reagent added to surface where phenylpyruvic acid reacts wit ferric ion & turns green at surface (pos) neg=clear

Hint 33

catabolism of proteins

Front 34

identify this test

Back 34

urease test.Tests for urease enzyme using urea agar.pos=fuschia color due to ph change in d medium towards d alkaline side.Neg=yellow or orange/yellow (acid)

Hint 34

catabolism of proteins

Front 35

identify this test

Back 35

citrate Test tests for d enzyme citrate permease.Simmon's citrate agar.pos=blue due to⬆ in alkaline
neg:remains green

Hint 35

we used a small inoculate(needle) 4 dis lab, cos a large inoculum(loop)can keep growing on itself