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67 Cards in this Set
- Front
- Back
*_________&_________
are still alive in bought milk. |
streptococcus & Lactobacillus
|
|
*__________ breakdown lactose into lactic acid and cause milk to sour
|
Lactobacillus
|
|
*2 bacterias found in chicken
|
Salmonella
Campylobacter |
|
* streptococcus is
G+ or G- shape color |
G+
coccus in chains tan |
|
*Lactobacillus is
G+ or G- shape color |
G+
rods in chains tan |
|
*Pseudomonas
G+ or G- shape |
G-
individual cells |
|
*Campylobacter
G+ or G- shape |
G-
individual rods |
|
*Salmonella
G+ or G- shape |
G-
individual rods |
|
Scientific Method
5 steps |
accumulate previous data
formulate hypothesis conduct experment report results conclusion |
|
independent variable
|
variable who's effects are being measured (Saccharin)
|
|
*Dependent Variable
|
Might be affected by the dependent variable (Bladder Cancer)
|
|
*Control group & Experimental group
|
Same conditions except the CG get no indep. var. and the EG get the indep. var. (Rats)
|
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*Controled Variable
|
All living conditions except indpen. var. (saccharin) ( age, health, rats per cage)
|
|
*Phenol
|
antiseptic
(throal lozenges) |
|
*Hexa chloro phene
|
antiseptic
(soap and detergent) |
|
*Cresol
|
Not antiseptic
(Lysol) |
|
*Alcohol
|
antiseptic
(scope, skin, & listerine) |
|
*Chlorine
|
antiseptic in small amounts
(swimming pools) |
|
*Iodine
|
antiseptic
(skin) |
|
*Hydrogen Peroxide
|
antiseptic
(cuts) |
|
*Enrlich tried to find a cure for_____, invented _______.
|
Syphillis
Salvarson |
|
*Salvarson
|
1st antimicrobial drug, it had some success
|
|
*Fleming studied ____ and accidentally discovered ________ _________
|
Staph Aureus
Penicillin notatum (PCN) |
|
*Florey seperated _________ from ______ and was able to provide the 1st antibiotic to the public.
|
PCN
mold |
|
*cell wall synthesis (antibio)
ex: APB |
prevent cell wall from forming.
ampicillin, pcn, bactitracin |
|
*PCN kill G ? better
why? |
positive
disrupt pepidoglycan |
|
*Protein synthesis (antibio)
Ex: GET |
prevent the making of protein
Gentamicin, Erythromycin, tetracycline |
|
*Eruthromycin kills G ? well
Tetracycline kill G ? well |
positive
positive and negative |
|
*Plasma membrane (antibiotic)
Ex: PNZ |
dirupt plasma membrane
Polymyxin, Nystatin, Zyvox |
|
*Nucleic Acid Synthesis (antibio)
Ex: AR |
provent formation of nucleic acid (DNA & RNA)
Actinomycin, Rifampin |
|
*Kirby Bauer
|
susceptibility test
|
|
*McFarland Tube
|
standard concentration of bacteria
|
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*Double streak
|
2 test on 1 agar plate in kerby bauer testing
|
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*Resistant (results)
|
don't use
no clear zone |
|
* (results) Susceptible
|
use
large clear zone |
|
* (results) Indeterminante
|
not sure
need more testing |
|
* (results) Moderately Susceptible
|
use in higher doses if can be used safely or concentrate antibiotic in the region of the infection.
|
|
* in MIC
tube 1-7 are |
expermental group with different doses of antibiotics in each.
|
|
*in MIC tube 8 should be
|
cloudy because it determine that the bacteria was alive to start with
|
|
*in MIC tube 9 should be
|
clear, because it determine if its contaminated
|
|
* in tube 1-7 what added
|
nutrient broth
bacteria antibiotics |
|
*in tube 8 whats added
|
nutrient broth
bacteria |
|
*in tube 9 whats added
|
nutrient broth
antibiotic |
|
*MIC doses concentration should be
|
X3
|
|
*Blood contain __% plasma & __% formed elements
|
55
45 |
|
*Blood plasma contain __% water & __% dissolve substances
|
90
10 |
|
*Leukocytes are ___ X's bigger that red blood cells and they
|
2-3
fight off infection, have a nucleus and DNA |
|
*Erythrocytes transports
|
O2 for electron transport and COe from cells to the lungs.
They don't have nucleus or DNA |
|
*Kerb Cycle produce
|
lots of CO2
|
|
*Hemoglobin is a
Hb + 4 O2 ___Hb(O2)4 lungs -----capillaries |
red protein found only in red blood cells.
|
|
*Hemoglobin carries
|
4 - O2's
|
|
*Red blood cells have ___ molecules of Hb and ___ molecules of O2.
|
200 million
800 million |
|
*what is total white count?
|
total number of WBC's in mm3
|
|
*Normal white count
slight elevation mean very elevated mean low mean |
5 to 10 thousand/ mm3
infection lukemia effects from med or HIV |
|
*Neutrophil __%
what do they do? if elevated? |
60-70
engulf bacteria bacterial infection |
|
*Lymphocytes __%
What do the do? If elevated? |
23-30
make up immune system viral infection |
|
*Monocytes _%
what do they do? If elevated? |
4-10%
become macrophage that engulf bacteria and virsuses Listeriosis |
|
*Esionphil _%
what do they do? If elevated? |
1-5
engluf antigens antibodies complex and attack parasite allergic reaction of parasite infection |
|
*Basophiles___%
what do they do? If elevated? |
.5-1
release histamines leukemia |
|
*Differential white count
|
5 types of WBC and %'s.
Dr. compares with normal range. |
|
*Exp.Results Bacillus Subtilis:
Lysol Bleach Listerine Scope |
L-24mm
B-24mm Lis-0mm s-0mm |
|
*Exp. Results Staph Epidermis
Lysol Bleach Listerine Scope |
L-36mm
B-40mm Lis-0mm S-17mm |
|
*Flaming endospores
|
1 sec +
5 sec - |
|
*Boiling water
Bacillius Subtilis E. Coli |
150 colonies
0 colonies |
|
*20% NaCl Solution
Staphylococcus epidermidis E. Coli |
250 colonies survives
80 colonies dies |
|
*Exp. Results E. Coli
PCN- Erthromycin- Tetracycline |
P- 9 mm
E- 9 mm T-26 mm |
|
*Exp. Results Staph epidermidis
PCN- Erthromycin- Tetracycline |
P-43 mm
E-28 mm T-36 mm |