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67 Cards in this Set

  • Front
  • Back
*_________&_________
are still alive in bought milk.
streptococcus & Lactobacillus
*__________ breakdown lactose into lactic acid and cause milk to sour
Lactobacillus
*2 bacterias found in chicken
Salmonella
Campylobacter
* streptococcus is
G+ or G-
shape
color
G+
coccus in chains
tan
*Lactobacillus is
G+ or G-
shape
color
G+
rods in chains
tan
*Pseudomonas
G+ or G-
shape
G-
individual cells
*Campylobacter
G+ or G-
shape
G-

individual rods
*Salmonella
G+ or G-
shape
G-
individual rods
Scientific Method

5 steps
accumulate previous data
formulate hypothesis
conduct experment
report results
conclusion
independent variable
variable who's effects are being measured (Saccharin)
*Dependent Variable
Might be affected by the dependent variable (Bladder Cancer)
*Control group & Experimental group
Same conditions except the CG get no indep. var. and the EG get the indep. var. (Rats)
*Controled Variable
All living conditions except indpen. var. (saccharin) ( age, health, rats per cage)
*Phenol
antiseptic
(throal lozenges)
*Hexa chloro phene
antiseptic
(soap and detergent)
*Cresol
Not antiseptic

(Lysol)
*Alcohol
antiseptic

(scope, skin, & listerine)
*Chlorine
antiseptic in small amounts

(swimming pools)
*Iodine
antiseptic

(skin)
*Hydrogen Peroxide
antiseptic

(cuts)
*Enrlich tried to find a cure for_____, invented _______.
Syphillis

Salvarson
*Salvarson
1st antimicrobial drug, it had some success
*Fleming studied ____ and accidentally discovered ________ _________
Staph Aureus

Penicillin notatum (PCN)
*Florey seperated _________ from ______ and was able to provide the 1st antibiotic to the public.
PCN
mold
*cell wall synthesis (antibio)

ex: APB
prevent cell wall from forming.

ampicillin, pcn, bactitracin
*PCN kill G ? better

why?
positive

disrupt pepidoglycan
*Protein synthesis (antibio)

Ex: GET
prevent the making of protein

Gentamicin, Erythromycin, tetracycline
*Eruthromycin kills G ? well

Tetracycline kill G ? well
positive

positive and negative
*Plasma membrane (antibiotic)

Ex: PNZ
dirupt plasma membrane

Polymyxin, Nystatin, Zyvox
*Nucleic Acid Synthesis (antibio)

Ex: AR
provent formation of nucleic acid (DNA & RNA)

Actinomycin, Rifampin
*Kirby Bauer
susceptibility test
*McFarland Tube
standard concentration of bacteria
*Double streak
2 test on 1 agar plate in kerby bauer testing
*Resistant (results)
don't use

no clear zone
* (results) Susceptible
use

large clear zone
* (results) Indeterminante
not sure

need more testing
* (results) Moderately Susceptible
use in higher doses if can be used safely or concentrate antibiotic in the region of the infection.
* in MIC
tube 1-7 are
expermental group with different doses of antibiotics in each.
*in MIC tube 8 should be
cloudy because it determine that the bacteria was alive to start with
*in MIC tube 9 should be
clear, because it determine if its contaminated
* in tube 1-7 what added
nutrient broth
bacteria
antibiotics
*in tube 8 whats added
nutrient broth
bacteria
*in tube 9 whats added
nutrient broth
antibiotic
*MIC doses concentration should be
X3
*Blood contain __% plasma & __% formed elements
55

45
*Blood plasma contain __% water & __% dissolve substances
90

10
*Leukocytes are ___ X's bigger that red blood cells and they
2-3
fight off infection, have a nucleus and DNA
*Erythrocytes transports
O2 for electron transport and COe from cells to the lungs.

They don't have nucleus or DNA
*Kerb Cycle produce
lots of CO2
*Hemoglobin is a

Hb + 4 O2 ___Hb(O2)4
lungs -----capillaries
red protein found only in red blood cells.
*Hemoglobin carries
4 - O2's
*Red blood cells have ___ molecules of Hb and ___ molecules of O2.
200 million

800 million
*what is total white count?
total number of WBC's in mm3
*Normal white count
slight elevation mean
very elevated mean
low mean
5 to 10 thousand/ mm3
infection
lukemia
effects from med or HIV
*Neutrophil __%
what do they do?
if elevated?
60-70
engulf bacteria
bacterial infection
*Lymphocytes __%
What do the do?
If elevated?
23-30
make up immune system
viral infection
*Monocytes _%
what do they do?
If elevated?
4-10%
become macrophage that engulf bacteria and virsuses
Listeriosis
*Esionphil _%
what do they do?
If elevated?
1-5
engluf antigens antibodies complex and attack parasite
allergic reaction of parasite infection
*Basophiles___%
what do they do?
If elevated?
.5-1
release histamines
leukemia
*Differential white count
5 types of WBC and %'s.
Dr. compares with normal range.
*Exp.Results Bacillus Subtilis:
Lysol
Bleach
Listerine
Scope
L-24mm
B-24mm
Lis-0mm
s-0mm
*Exp. Results Staph Epidermis
Lysol
Bleach
Listerine
Scope
L-36mm
B-40mm
Lis-0mm
S-17mm
*Flaming endospores
1 sec +

5 sec -
*Boiling water
Bacillius Subtilis

E. Coli
150 colonies

0 colonies
*20% NaCl Solution
Staphylococcus epidermidis

E. Coli
250 colonies survives

80 colonies dies
*Exp. Results E. Coli
PCN-
Erthromycin-
Tetracycline
P- 9 mm
E- 9 mm
T-26 mm
*Exp. Results Staph epidermidis
PCN-
Erthromycin-
Tetracycline
P-43 mm
E-28 mm
T-36 mm