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40 Cards in this Set
- Front
- Back
What is a positive result for an Urea test
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Red/Cerise
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What is a negative result for an Urea test
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yellow
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What is the theory of the Urea test?
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Does organism have urease enzyme
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What is the Urea test good for?
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-Proteus
-Providencia -Morganella |
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What is a positive result of Gelatin Hydrolysis test?
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Agar becomes liquid when cold
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What do you add to the urea test for results to be viewed?
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nothing
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What is a negative result of the Gelatin Hydrolysis test?
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Agar remains solid when cold
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What is the theory in the Gelatin Hydrolysis test?
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Looking for the enzyme GELATINASE
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What do you need to add for results of the starch hydrolysis(starch agar)?
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Gram's Iodine
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What is a positive result of the Starch Hydrolysis (starch agar)?
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clear zone in agar around colony
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What is a negative result of the Starch Hydrolysis (starch agar)?
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No clear zone in agar
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What is the theory of the Starch Hydrolysis(starch agar) test?
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Does organism have enzyme amylase
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What do you need to add for results of the Casein Hydrolysis(skim milk agar)
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nothing
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What is a positive result in Casein Hydrolysis(skim milk agar)
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Clear zone in agar around colony
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What is a negative result in Casein Hydrolysis(skim milk agar)
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No clear zone
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What is the theory of the Casein Hydrolysis(skim milk agar)test?
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Does organism have casenase
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What do you need to add to read the Citrate Use(Simmons citrate agar slant)?
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Nothing
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What is a positive result in the Citrate Use(Simmons citrate agar slant)test?
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Prussian blue
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What is a negative result of the Citrate Use(Simmons citrate agar slant)test?
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no color change (so green)
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What is the theory behind the Citrate Use(Simmons citrate agar slant)test?
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Can organism use citrate
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What is Citrate Use(Simmons citrate agar slant) test good for?
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Enterbacter aergenes
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What are the contents of the litmus milk reactions?
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-10% skim milk(protien source
-Litmus(pH indicator) |
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What is a positive reaction in Litmus milk test for ACID RXN(fermentation)
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pink broth
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What is a positive reaction in Litmus milk test for ALKALINE RXN?
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blue broth
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What is a positive reaction in Litmus milk test for LITMUS REDUCTION (e reduce litmus)
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White media
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What is a positive reaction in Litmus milk test for COAGULATION?
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solid media
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What is a positive reaction in Litmus milk test for PEPTONIZATION(protein digestion)
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translucent brown media
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What is a positive reaction in Litmus milk test for ROPINESS?
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thick slime on bottom of tube
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What do you need to add to Trytophan hydrolysis (SIM AGAR TUBE).......INDOLE test?
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Kovac reagent
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What is a positive result for Trytophan hydrolysis (SIM AGAR TUBE).......INDOLE test?
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Cherry Red ring
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What is a negative result for Trytophan hydrolysis (SIM AGAR TUBE).......INDOLE test?
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NO red ring
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What is the theory for Trytophan hydrolysis (SIM AGAR TUBE).......INDOLE test?
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looking for indole
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What is Trytophan hydrolysis (SIM AGAR TUBE).......INDOLE test good for?
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E. coli
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What do you need to add to the Hydrogen sulfide producion(SIM AGAR TUBE) ....H2S test?
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nothing
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What is a positive result for Hydrogen sulfide producion(SIM AGAR TUBE) ....H2S test?
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Black precipitate
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What is a negative result for Hydrogen sulfide producion(SIM AGAR TUBE) ....H2S test?
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NO black precipitate
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What is the theory for Hydrogen sulfide producion(SIM AGAR TUBE) ....H2S test?
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Looking for H2S
cysteine desulfurese enzyme |
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What is test for Hydrogen sulfide producion(SIM AGAR TUBE) ....H2S good for?
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Proteus vulgaris
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What does the Urea hydrolysis contain?
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Urea
Phenol Red pH indicator |
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What is the rxn that happen in the casein hydrolysis?
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protein breakdown
proteolysis/peptonization |