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88 Cards in this Set

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mycelium
collective growth of hyphae
Saprotrough
eat decaying matter
Necrotrough
eat dead matter
biotrough
eat living matter but don't kill it
What do we grow fungi on?
PDA - Potatoe dextrose Agar
Antimicrobials
reduce or kill microbes
Physical methods to kill microbes
high or low temp, pH, irradiation and fitration
Disinfectants
chemical compounds that are used on non-living objects (lysol, detergent and bleach)
Antiseptics
chemicals used on living tissue (hydrogen peroxide, rubbing alcohol, neosporin)
Antimicrobials
selectively kill or inhibit pathogens inside the host (antibiotics and synthetic antibiotics)
disk diffusion method
Kirby bauer
What's measured in Kirby bauer?
the zone of inhibition
What agar is the kirby bauer test?
Mueller-Hinton agar - which doesn't contain PABA that would throw off the test
Name of the turbidity standard
McFarland standard
Blood Agar
differential but not selective. 5% sheeps blood
Catalase test
tests to see if bac can convert H2O2 to H2O +O2.
MSA
S + D. mannitol fermenting shown by phenol red turning yellow. Salt env - growth on plate. Only grows gram+
MacConkey
S+D. Only grows gram -. Will grow all gram -, only lactose fermenters will turn the agar red (neutral red)
Eosin Methylene Blue
S+D. only grows gram -. lactose fermentors will turn black. Strong lactose fermenters will turn it metallic green black.
Salmonella-Shigella
S+D. Only grows salmonella and Shigella. Will show red for lactose fermenters. Will show black for lactose fermenters and H2S gas
TSI slant
uses phenol red. Tests for H2S production, produce gas from fermentation, and fermentation of glucose, lactose and sucrose. Slant/Butt/gas/H2S
Positive for Caseinase
B. subtilis
Positive for starch hydrolysis
Bacillus subtilis
Positive for gelatinase
Staphylococcus aureus
EMB - metallic green results
Escherica coli and Klebsiella pneumoniae
EMB - Colorless colonies/coloring of medium
Salmonella typhimurium and Yersinia enterocolica
SS results
Colorless colonies with black centers. E. coli and K. pneumoniae pink colonies. y. enterocolitca - colorless colonies.
MacConkey results
E. coli and K. pneumoniae - pink colonies. S. typhimurium and Y. enterocolitica - colorless colonies
TSI slant results A/A/v/-
E. coli and K. pneumoniae
TSI slant results K/A/-/+
S. typhimurium
TSI slant results K/A/v/-
Y. enterocolitica
Positive for citrate
S. typhimurium and K. pneumoniae
Non hemolysis in Blood agar
S. epidermis
Alpha hemolysis in Blood agar
Streptococcus pneumoniae
Beta hemolysis in Blood agar
Streptococcus pyogenes and Staphylococcus aureus
Positive for Catalase
Staphylococcus aureus and Staphylococcus epidermis
What grows on MSA?
Staphylococcus aureus - medium turns yellow. Staphylococcus epidermis - growth, no color change
T4r only infects what bac?
E. coli
Dye used to prepare fungi slide
Methylene Blue
Phenol red is used in both ___ and ___ agars
MSA and TSI
Neutral red is used in both ___ and ___ agars
MacConkey and SS
What gives Alpha hemolysis, neg catalase and NaCl intolerant?
Streptococcus pneumoniae
What does a positive result for citrate test look like?
blue
Unknown 1, 2, 3
Y. enterocolitica, S. pyogenes, S. aureus
mycelium
collective growth of hyphae
Saprotrough
eat decaying matter
Necrotrough
eat dead matter
biotrough
eat living matter but don't kill it
What do we grow fungi on?
PDA - Potatoe dextrose Agar
Antimicrobials
reduce or kill microbes
Physical methods to kill microbes
high or low temp, pH, irradiation and fitration
Disinfectants
chemical compounds that are used on non-living objects (lysol, detergent and bleach)
Antiseptics
chemicals used on living tissue (hydrogen peroxide, rubbing alcohol, neosporin)
Antimicrobials
selectively kill or inhibit pathogens inside the host (antibiotics and synthetic antibiotics)
disk diffusion method
Kirby bauer
What's measured in Kirby bauer?
the zone of inhibition
What agar is the kirby bauer test?
Mueller-Hinton agar - which doesn't contain PABA that would throw off the test
Name of the turbidity standard
McFarland standard
Blood Agar
differential but not selective. 5% sheeps blood
Catalase test
tests to see if bac can convert H2O2 to H2O +O2.
MSA
S + D. mannitol fermenting shown by phenol red turning yellow. Salt env - growth on plate. Only grows gram+
MacConkey
S+D. Only grows gram -. Will grow all gram -, only lactose fermenters will turn the agar red (neutral red)
Eosin Methylene Blue
S+D. only grows gram -. lactose fermentors will turn black. Strong lactose fermenters will turn it metallic green black.
Salmonella-Shigella
S+D. Only grows salmonella and Shigella. Will show red for lactose fermenters. Will show black for lactose fermenters and H2S gas
TSI slant
uses phenol red. Tests for H2S production, produce gas from fermentation, and fermentation of glucose, lactose and sucrose. Slant/Butt/gas/H2S
Positive for Caseinase
B. subtilis
Positive for starch hydrolysis
Bacillus subtilis
Positive for gelatinase
Staphylococcus aureus
EMB - metallic green results
Escherica coli and Klebsiella pneumoniae
EMB - Colorless colonies/coloring of medium
Salmonella typhimurium and Yersinia enterocolica
SS results
Colorless colonies with black centers. E. coli and K. pneumoniae pink colonies. y. enterocolitca - colorless colonies.
MacConkey results
E. coli and K. pneumoniae - pink colonies. S. typhimurium and Y. enterocolitica - colorless colonies
TSI slant results A/A/v/-
E. coli and K. pneumoniae
TSI slant results K/A/-/+
S. typhimurium
TSI slant results K/A/v/-
Y. enterocolitica
Positive for citrate
S. typhimurium and K. pneumoniae
Non hemolysis in Blood agar
S. epidermis
Alpha hemolysis in Blood agar
Streptococcus pneumoniae
Beta hemolysis in Blood agar
Streptococcus pyogenes and Staphylococcus aureus
Positive for Catalase
Staphylococcus aureus and Staphylococcus epidermis
What grows on MSA?
Staphylococcus aureus - medium turns yellow. Staphylococcus epidermis - growth, no color change
T4r only infects what bac?
E. coli
Dye used to prepare fungi slide
Methylene Blue
Phenol red is used in both ___ and ___ agars
MSA and TSI
Neutral red is used in both ___ and ___ agars
MacConkey and SS
What gives Alpha hemolysis, neg catalase and NaCl intolerant?
Streptococcus pneumoniae
What does a positive result for citrate test look like?
blue
Unknown 1, 2, 3
Y. enterocolitica, S. pyogenes, S. aureus