Micro Lab Practical 2

Front 1

Catalase

Back 1

breaks down peroxide into H2O and O2

Front 2

Catalase reaction with Staph.

Back 2

positive

Front 3

Catalase reaction with Strep.

Back 3

negative

Front 4

What type of plate is manitol salt agar

Back 4

selective (contains
NaCl) and differential
(contains mannitol)

Front 5

What bacteria shows positive reaction to MSA?

Back 5

S. aureus ferments
mannitol-yellow halo

Front 6

What staph infection is coagulase positive?

Back 6

staph aureus

Front 7

What staph infection in coagulase negative?

Back 7

staph epi.

Front 8

blood agar

Back 8

– enriched media
– fastidious organisms

Front 9

Beta hemolysis seen on blood agar

Back 9

– blood cells are
completely destroyed
– S. pyogenes

Front 10

Alpha hemolysis seen on blood agar

Back 10

– red blood cells are
partially destroyed
– Medium turns olive
green to brown
– S. pneumonia

Front 11

Gama hemolysis seen on blood agar

Back 11

no hemolysis visible

Front 12

What is a mitus-salivarius medium?

Back 12

medium used to grow oral cavity strep.

Front 13

What does mitus salivarius medium contain?

Back 13

tellurite

Front 14

What color will the colonies be on a mitus salivarius medium

Back 14

blue

mitis will produce pinpoint colonies

salivarius will produce gumdrop colonies

Front 15

What is chocolate agar?

Back 15

– cooked blood agar
– enriched media (not
selective or
differential)

Front 16

what bacteria is a chocolate agar used on?

Back 16

neisseria

Front 17

What are the major families of enteric bacteria?

Back 17

– Salmonella
• typhoid fever
– Shigella
• shigellosis
– Escherichia
• traveler’s diarrhea

Front 18

Is neisseria oxidase positive or negative?

Back 18

positive

Front 19

What is MacConkeys agar used for ?

Back 19

enteric bacteria

Front 20

What are the characteristics of MacConkeys agar?

Back 20

Selective
– inhibits growth of Gram
(+)
• Bile salts
• Crystal violet
• Differential
– lactose
• Lactose Fermenters
– pink to brick red colonies

Front 21

What are the characteristics of Eosin-methylene Blue agar?

Back 21

• Selective
– dyes inhibit Gram (+)
• Differential
– lactose
• Lactose Fermenters
– pigmented colonies
• Non-Fermenters
– colorless colonies

Front 22

What does EMB agar do to E. coli?

Back 22

Black colonies eith a green metalic sheen

Front 23

What is the effect of Ultra-Violet Light to bacteria?

Back 23

• causes production of thymine dimers
– DNA incapable of base pairing
– prevents transcription of DNA into RNA
• Protein synthesis interrupted = death
• Lethal and non-lethal mutations

Front 24

What is the wavelength of uv light used to mutate bacteria?

Back 24

• Wavelength of 265 nanomenters

Front 25

What type of agar do fungi prefer? And Name

Back 25

• low pH & high sugar concentration

- Sabouraud’s Dextrose Agar

Front 26

Deuteromycota

Back 26

• Penicillum
• Asexual Reproduction
– conidiospores

Front 27

Deuteromycota

Back 27

Aspergillus
– Asexual reproduction
• conidiospores

Front 28

Basidiomycota (Club Fungi)

Back 28

• Mushrooms
• Asexual spores
– Conidiospores
• Coprinus
• Sexual spores
– Basidiospores

Front 29

Zygomycota (bread mold)

Back 29

• FamilyRhizopus
– conjugation fungi
• Asexual reproduction
– sporangiosporess

Front 30

Rhizopus

Back 30

• Asexual reproduction
– sporangiosphores

Front 31

Ascomycota (sac fungi)

Back 31

• Ascospores
– 8 in ascus