Micro 2-2, 2-3, 2-4, 3-6

Front 1

What 2 things determine Colony morphology/appearance?

Back 1

Environmental factors and genetic makeup

Front 2

What are the 4 genetic makeup factors

Back 2

Color, size, shape, and texture

Front 3

What purpose do agar plates have in bacterial growth morphologies?

Back 3

Agar plates help with the organism identification process

Front 4

How does a colony form?

Back 4

When a single bacterial cell is deposited on a solid nutrient medium, it begins to divide. One cell makes two, two makes four, four make eight. Eventually a visible mass of cells, a colony, appears.

Front 5

In what 4 ways are color, size, shape, and texture of microbial growth determined?

Back 5

1. genetic makeup of the organism 2. nutrient availability 3. temperature 4. incubation

Front 6

The 5 basic categories of colony morphology

Back 6

1. colony shape 2. margin (edge) 3. elevation 4. texture 5. pigmentation (color)

Front 7

6 types of colony shape

Back 7

Punctiform, irregular, circular, rhizoid, spindle, filamentous

Front 8

5 types of margin

Back 8

1. Smooth round/entire (smooth with no irregularities)

2. undulate (wavy)

3. lobate (lobed)

4. filamentous

5. rhizoid (branched like roots)

Front 9

5 elevations of colonies

Back 9

1. flat

2. raised

3. convex

4. pulvinate (very convex)

5. umbonate (raised in the center)

Front 10

colony counter

Back 10

used to view subtle differences in colony shape and size

Front 11

what 2 things allow greater observation of detail in a colony counter?

Back 11

1. transmitted light

2. magnifying glass

Front 12

colony counter is best determined with ______.

Back 12

reflected light

Front 13

The grid in the colony counter background is a ______.

Back 13

counting aid

Front 14

Classify.

Back 14

1. raised

2. raised, spreading edge

3. flat, raised margin

4. growth into medium

Front 15

Classify 2.

Back 15

1. convex 2. umbonate 3. plateau 4. flat

Front 16

Classify.

Back 16

1. Smooth, entire

2. rhizoid

3. irregular (erose)

4. lobate

5. filamentous

Front 17

4 types of descriptions used to describe colony morphology:

Back 17

1. color

2. surface characteristics

3. consistency

4. optical properties

Front 18

2 types of surface characteristics

Back 18

1. shiny

2. dull

Front 19

6 types of consistency

Back 19

1. dry

2. moist

3.mucoid

4 . wrinkled

5. Smooth

6. rough

Front 20

3 types of optical properties

Back 20

1. opaque 2. translucent 3. transparent

Front 21

6 colonies studied in this lab

Back 21

1. micrococcus luteus 2. corynebacterium xerosis 3. lactobacillus plantarum 4. mycobacterium smegmatis 5. bacillus subtilis 6. proteus miabilis

Front 22

Colony elevation and texture are mostly easily observed with?

Back 22

Reflected light: hitting the growth at a low angle

Front 23

Colony opacity is best seen with?

Back 23

Transmitted light not reflected

Front 24

Purpose of an Agar slant

Back 24

•Agar slants are generally used for cultivation & maintenance of stock cultures

1.More difficult to contaminate compared to a plate

2.Do not dry out quickly

3.Require less media than plates

Front 25

The usual shape of Agar slant growth.

Back 25

•Most slant growth = filiform

-Dense,opaque growth with smooth edges

Front 26

3 Agar slant growth patterns

Back 26

1.Spreading edge from motile organisms

2. Friable (crusty) growth –many

3. Translucent / transparent growth

Front 27

3 Growth Patterns in Broth

Back 27

•Pellicle: Organisms float on top of the medium & produce a membrane

•Sediment: Organisms sink to the bottom

•Flocculent growth: Organisms clump together

Front 28

Why do we not shake the test tube of nutrient broth before looking?

Back 28

•Do not agitate nutrient broth prior to looking… sediment becomes uniform fine turbidity!