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20 Cards in this Set
- Front
- Back
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what are the possible tsi test results and interpretations
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1.yellow/yellow= glucose+ lactose or sucrose
2.red/yellow= glucose fermintation by acid production 3.red/red= no fermentation 4.black= H2S sulfer reduction 5. cracks= gas production |
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What does beta hemolysis mean
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a clear area around the bacteria growth that indicates lysis of the red blood cells.
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what is the indole test
what is indicator what does it mean |
1. it is a test to see what if a bacteria is capable of producing indole using the enzyme tryptophanase.
2. add kovaks reagent- if it forms a red ring it is positive. 3. indicates a positive reaction and presence of trypophanase. |
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what is the citrate test?
what is indicator what does it mean |
1. test to see if the organism has the ability to use citrate as its sole source of carbon.
2.bromthymol blue dye is added as an indicator turn blue at a high pH and green at a low pH, blue is citrate positive. 3, means that it can use citrate as its sole source of carbon. |
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what is a condensor
what is it used for how is it controlled |
1.found under the stage used to collect and concentrate light passing upward from the light source into the lens systems.
2. controlled by the condensor height adjustment. |
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what does the lever on the iris do
how does kohlering relate to this |
1.regulates the amount of light entering the condensor ti get good contrast.
2.kohleringi is a process of closing the field diaphram ring on very bottom until its almost closed, through the oculars only a small amount of light will appear. raise and lower condenser until the edges of the circle of light come into sharp focus-- this represents optimal optical adjustment of the condenser. |
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what are the lens called
which is high and dry which is oil immersion which is low |
1. objectives
2, 40x 3, 100x 4, 4x |
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what is the ring called on one of the eyepieces
what is it used for why doesn't the other side have one |
1. called the diopter focusing ring
2, used to focus the eyepiece onside for that eye 3. the other side uses fine focus for adjustment |
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what hold the slide on the stage
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the speciman holder
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where are the x and y axis knobs
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they are hanging down from the stage
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what is the mannitol salt agar test?
what is indicator what does it mean |
1. used to isolate different staph organisms
2. indicator is phenol red in agar turns yellow at low pH and red at high pH, it has salt in it and most other bacteria can't survive in it. So, if growth it means it is salt tolerant. If it turns yellow it means its fermenting mannitol shown by its acid production turning the phenol red indicator yellow. |
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what is the nitate reduction test?
what is indicator what does it mean |
1. testing to see if the organism has the ezyme to ruduce nitrate to nitrite.
2, start off with clear nitrate broth incubate, then add 2 drops of sulfanilic acid and 2 drops of alpha napthylamine check for color change to red. Red on the first step means reduction and pos 1st step test, if no color change add zinc if it turn red then it is a 2nd step negative test, if colorless then positive for 2nd step for reducing nitrate to a more reduced state. |
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what is the instrument called to dispense fluid from pipetts
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pipetman.
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what is the urea hydrolysis test?
what is indicator what does it mean |
1. tests to see if the organism can breakdown urea into ammonia and c02.
2, it has phenol red in it. if amonia is produced is causes the pH to rise turning the media bright pink for a positive urea test. no change is a negative test, |
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On the UV light expirement:
The plate on the right was covered with its lid throughout the experiment. The plate on the left was have covered with a paper towel. Explain what happened. Whaich side of the plate on the left was covered with the paper towel? |
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Fungi- ques 12
These two pictures represent two different views of the same organism. What kind of organism is this? What are the small round structures, labeled "a," in the right picture? What are the clear filamentous structures called? (labeled "b"). |
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Fungi- ques 13
All three of these pictures are of the same organism. What kind of organism is this? What are the structures labeled "a" called? What are the filaments labeled "b" called? What the tiny crosswalls labeled "c" called? Can you identify the species of this speciman? |
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Ques 14
These two images are from different parts of the same slide. Obviously the image on the right has more cells in it. In the image on the left the cells are labeled with a small letter "a." The magnification is 1000x total. What kind of a stain is this? What does the white halo around the bacterial cells represent? What is the function of this structure? What is its role in disease? |
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Ques 15-
This wet mount is magnified 1000x. What is the name of this organism? What are the round, green structures called? what are these structures used for? What are the white cross-walls? What does each rectangular unit represent? |
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Ques 17-
Both of the above pictures are wet mounts of the same organism. Magnification is 450x. What is the name of this organism? What is happening to the cells labeled "A" in either picture? Why is this organism important? |
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