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15 Cards in this Set
- Front
- Back
TSIA
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*triple sugar iron agar
*big tube slant red in color *differentiates member of enterobacteriaciae and other gram - rods. |
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TSIA results
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*yellow slant/yellow butt = glucose and lactose and/or sucrose fermentation.
*red slant/yellow butt = glucose fermentation only *red slant/red butt = no ferm. |
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Oxidase
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*Differentiates between organisms that contain cytochrome c oxidase and those that do not.
*Indicator for Neisseria bacteria, and enterobacteriaciae(-) vs. pseudomonas(+) |
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Oxidase results
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a dramatic color change to blue within 20 seconds indicated a positive test.
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MIO
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Motile or non-motile?
Indole - does the org. have tryptophanase? Ornithine - forming alkaline by products. |
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MIO results
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Motile = cloudy, NM - you can see the original stab line.
Indole = is there a red ring layer on top. Ornithine - if bromocresol blue changes from yellow to purple then its (+) |
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Citrate
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*dark green small slant tube
*Indicator is bromothymol blue *Can org. use citrate as sole source of Carbon? *Enterobacteriaciae & gram (-) rods |
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Citrate results
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(+) if even small amount of blue is present or growth is present.
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LIA
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*lysine iron agar, big purple slant
*looks for enteric bacteria's ability to decarboxylase or deaminase lysine *Salmonella and Shigella |
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LIA Results
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*P/P = lysine deaminase (-) and lysine decarboxylase (+)
*P/Y = lysine deaminase (-), lysine decarboxylase (-) and glucose fermentation. *R/Y = lysine deaminase (+), lysine decarboxylase (-) and glucose fermentation. *black precipitate = sulfur reduction |
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Coagulase
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*milky white color
*differentiates staphylococcus aureus from other gram (+) cocci. |
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Coagulase results
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(+) =within 24 hours plasma has coagulated(thickened)
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OF Glucose
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*sealed w/ oil
*indicator = bromo blue; yellow @ 6.0 and green @ 7.1 |
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OF Glucose results
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*if sealed is green or blue and unsealed has any amount of yellow then its oxidation.
*if both sealed and unsealed are all yellow then either oxidation and fermentation or just oxidation took place. *if both are slightly yellow at tops then oxidation and slow fermentation or just slow ferm. took place. *If results are green or blue then no O or F took place. |
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MIO is a semi-solid medium
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Hippurate =
*Sodium hippurate is injected into tubes, and then 5 drops of Ninhydrin are added. incubate for ~20 minutes.(+) = purple color darker than control tubes color. |