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47 Cards in this Set
- Front
- Back
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Pasteurella+ mannheimia- speciment
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Portion of pneumonic lung, septicaemia: liver spleen kidney lymph nodes, pus, exudates, nasal swab, purulent nasal discharge, mastitis milk
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Pasteurella+ mannheimia- direct exam
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G- rods/coccobacilli
Fowl cholerae: bipolar staining in Giemsa stain |
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Pasteurella+ mannheimia- culture tech.
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Media- Blood agar + McC
Selective medium w. clindamycin for P. multocida Blood agar w. neomycin, bacitracin, actidione Incubation- 24-48 h, 37 degrees, aerobically |
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Pasteurella+ mannheimia- identification
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Colony morphology
Growth on McC Oxidase + Biochemical profile |
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P. multocida colony morphology
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Evident after 24h
medium, round, shiny, greyish, NON-haemolytic Type A- large mucoid colonies Smell- sweet NO growth on McC Indole + |
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M. haemolytica colony morphology
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Pinpoint, red colonies- on McC
No odor indole - |
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Haemophilus- speciment
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Swabs- nasal, throat, cervical
Cavity fluids Cerebrospinal fluid synovial fluid tissue fragments |
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Haemophilus- direct exam
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G- rods
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Haemophilus- isolation
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X (hemin) + V (NAD) factors must be supplied
Chocolate agar Staphylococcus on blood agar releases V factor into medium |
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Haemophilus and histophilus- identification
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Requires X + V factors
Growth enhancement of CO2 Catalase + oxidase reactions Carbohydrate utilization |
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Actinobacillus- speciment
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Pus, exudates from lesions, tissue biopsies, pneumonic lesions, granulomatous biopsies
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Actinobacillus- direct exam
for A. lingiersii infections |
Pus/exudates washed with dist. water in petri dish- small grey-white granules.
Granules are places in a drop of KOH on a slide, crushed with coverslip. Structure can be seen under low-power objective. Stained HP secretions also demonstrate characteristic clubs and club colonies |
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Actinobacillus- isolation
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Sheep blood agar
Chocolate agar for A. pleuropneumoniae (req. V) 37 degrees, 24-72h, improved growth by 5-20 % CO2 |
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Actinobacillus- identification
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Colony morphology
Biochemical tests CAMP test: A. pleu. enhance the haemolysis caused by beta-haem. of Staph. aureus |
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Enterobacteriaceae- speciment
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Depend on clinical signs
Intestinal- faeces, rectal swab, fragment of intestine Extraintestinal- blood, tissue, milk, urine, discharges, various exudates, food, carcases |
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Enterobacteriaceae- identification
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Colony morphology
Reaction to indicator/selective media Biochemical tests- OF-test, oxidase, triple sugar iron, lysine decarboxylation, IMViC, API ZOE Serotyping for detection of antigen Antibiotic sensitivity |
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Enterobacteriaceae- colony morphology
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Large, NON-haemolytic, shiny, greyish
Proteus spp.- swarm on blood and nutrient agar Klebsiella pneumoniae- mucoid colonies Serratia rubidea + marcescens- proc. red pigment |
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Escherichia coli- strains
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ETEC, EPEC, EHEC, NTEC, EIEC
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Escherichia coli- speciment
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faeces, urine, blood, tissue
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Escherichia coli- culture tech.
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BA, McC, McC with sorbitol
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Escherichia coli- identification
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Growth on McC (Lact +)
Presence of haemolysis Sorbitol hydrolysis Pure culture isolation- antibiotic susceptibility biochemical tests: Lact+, indo+, H2S +, Oxi- serotyping, PCR |
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Escherichia coli O157: H7- identification
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colorless colonies, pink colonies on McC with sorbitol
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Salmonella- speciment
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Post mortem- spleen, kidney, lymph nodes, liver with gall bladder, clinically changed tissue
Post slaughter exam: muscles, lymphnodes Clinical: blood, urine, bile, faeces, vomit Suspected animal feed food from animal origin |
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Yersinia- speciement
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lymphnodes, faeces, blood, biopsy of tissues
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Yersinia- isolation
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difficult, requires selective media
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Yersinia- culture tech.
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Blood agar, McC, XLD
irgasan, tricarcillin, chlorate broth incub.- 22 or 37 degrees Result: Biochemical identification antibiotic susceptibility |
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Water purity test
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Tested by looking for coliform bacteria- members of Esch. usually ferment lactose and produce acid + gas; E.coli, klebsiella, enterobacter
Methods: Multiple-tube dermentation membrane filter ONPG + MUG test |
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Staphylococcus- speciment
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pus, exudates, milk
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Staphylococcus- culture tech.
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Blood agar, selective agar w. colistin, nalidixic acid, McC
Incub.- anaerobically, 37 deg. 24-48 h |
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Staphylococcus- Identification
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Colony morph.- white, opaque, large
Haemolysis- S. aureus/pseudointermedius: alpha + beta, S. hyicus- no Absence of growth on McC Cat +, Oxi -, Coa +-(slidetubetest) Biochemical profile- purple agar, phage typing Antibiotic susceptibility OF test, API |
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Staphylococcus- direct exam
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G+ irregular clustered cocci
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Purple agar
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Contains 1% maltose + pH indicator (bromocresol purple)
Differentiates S. aureus and S. pseudointermedius: S. aureus- utilizes maltose + acidifies medium- change in color (yellow) S. pseudoi.- does not utilize maltose- no change |
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Slide tube test- coagulase test
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Staphylococcus mixed with rabbit plasma on slide/test tube
Fibrinogen in plasma converts to fibrin by coagulase Slide: detects BOUND coagulase,clumping factor (bound coagulase) on bacterial surface 1-2 min tube: detects FREE coagulase, clot forms after 24h 37 degree incub. |
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Streptococcus- speciment
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Pus, milk, exudates
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Streptococcus- culture techniques
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Blood agar, selective media
anaerobically, 37 degrees, 24-48h Sensitive to desication- place in transport medium directly |
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Streptococcus- identification
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colony morph.- translucent, small, may be mucoid
hamolysis Cat -, no McC growth Lancefield grouping, biochemical profile |
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Streptococcus- direct exam
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G + cocci in chains
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Streptococcus- differentiation
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haemolysis
lancefield grouping- serological latex agglutination- latex w. antibodies- aggl.=+ biochemical tests |
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Listeria- speciment
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meningoencephalitits- CSF, tissue from medulla+pons
abortion- foetal abomasal content, cotyledons, uterine discharge septicaemia- liver, spleen, blood |
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Listeria- direct exam
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G + rods/coccobacilli
IF technique Histopathological exam of brain tissue- microabscesses |
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Listeria- culture tech.
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Blood agar, McC, selective medium w. potassium tellurite
aerobic 37 d, 24-48 h Cold enrichment- brain tissue |
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Listeria- cold enrichment
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Brain tissue
Homogenization of medullary tissue 10 % supression- nutrient broth Incub. 4 degrees 12 weeks Subcultured onto blood agar |
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Listeria- identification
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Colony morph.- small, smooth, flat, blue-green (illum.)
Haemolysis- beta Cat +, Aesculin + Motility at 25 not at 37 induces keratoconjunctivitis- test on rabbits CAMP: + S. aureus - Rhodococcus equi used to identify L. monocytogenes |
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Erysipelothrix- speciment
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Blood
Necropsy: liver, spleen, kidney, heart, synovial tissue, skin |
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Erysipelothrix- direct exam
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Acute: slender G+ rods
Chronic: G + filaments |
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Erysipelothrix- culture tech.
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Blood agar
McC Selective media w. crystal violet, sodium azide aerobic, 37 d, 24-48h |
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Erysipelothrix- identification
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Colony morph.
24 h- non-haem, pinpoint 48 h- narrow zone alpha haem. No McC growth Cat -, Oxi-, H2S + Biochem tests Serotyping Virulence testing |
