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91 Cards in this Set
- Front
- Back
Crenate |
Hypertonic |
|
80-100 fL |
MCV ref range |
|
26-34 pg |
MCH ref range |
|
Swells and lyse |
Hypotonic solution |
|
32-37 g/dL |
MCHC ref range |
|
High MCHC |
Spherocytes mchc? |
|
11.5-14.5 % |
RDW ref range |
|
Anisocytosis |
RDW is directly proportional with? |
|
Females- 36-46% Males- 41-53% |
Hct ref range |
|
Hct |
%age of rbc in a given volume of whole blood |
|
Spun microhct |
Reference manual method for hct |
|
Mcv × rbc / 10 |
Hct formula |
|
Males- 13.5-17.5 g/dL Females-12-16 g/dL |
Hgb ref range |
|
150-450 x10^9 |
Plt ref range |
|
6.8-10.2 fP |
MPV ref range |
|
Wright's stain |
Most commonly used routine PBS stain |
|
Methylene blue Eosin |
Composition of Wright's stain |
|
Methanol fixative |
Used in the staining process to fix the cells to the slide |
|
Add phosphate buffer (6.4-6.8 pH) |
How to start stain |
|
Prussian blue |
Nonvital monochrome stain |
|
K ferrocyanide HCl Safranin (counter) |
Prussian blue composition |
|
Supravital stain |
New methylene blue (ppt RNA) Neutral red with BCG. (Counter) |
|
Heinz bodies |
Neutral red with brilliant cresyl green visualizes what |
|
G6PD |
Heinz bodies clinical disorder |
|
Mesoblastic (yolk sac) Hepatic Myeloid/medullary phase |
3 stages of intrauterine hematopoiesis |
|
Yolk sac/mesoblastic phase |
Begins at 19 days gestation |
|
Erythroblast |
First cell to be produced |
|
Portland, Gower I, Gower II |
Produced by erythroblast |
|
6 week gestation |
Start of hepatic phase |
|
Hgb F, A and A2 |
Hgb in hepatic phase |
|
Myeloid/medullary phase |
5th month of gestation |
|
Granulocytes |
Produced mainly in myeloid stage |
|
Myeloid Lymphoid |
2 progenitor cells |
|
CFU-MEG |
No IL3 |
|
CFU-Ba |
No GM-CSF |
|
Thrombopoietin, GM-CS Thrombocytes |
CFU-MEG |
|
GM-CSF, G-CSF, IL3 Neutrophils |
CFU GM, CFU-G |
|
GM-CSF, IL-3, M-CSF Monocyte |
CFU-GM CFU-M |
|
Erythropoietin, IL3, GM-CSF Erythrocyte |
CFU-E |
|
GM-CSF, IL3, IL5 Eosinophil |
CFU-Eo |
|
IL3, IL4 Basophil |
CFU-Ba |
|
Primary lymphoid tissue |
Ag-independent lymphopoiesis |
|
Granulocytes, monocytes |
Phagocytes of the leukocytes |
|
Lymphocytes, plasma cells and monocytes |
Immunocytes of leukocyte |
|
4-11 x10^9 |
Ref range of WBC |
|
Basophil Eo Neutro |
Granuloctes |
|
Neutrophils |
First to reach the tissue and phagocytize bacteria (They die in the process) |
|
Monocytes |
Differentiate into macrophage Do not die in the process |
|
T-lymph |
Cellular immunity Produce cytokines and IL |
|
B-lymph |
Develop into plasma cells and produce Ab; humoral immunity |
|
Natural killer cells |
Large granular lymphocytes Destroy tumor cells and viruses |
|
Eosinophils |
Modulate allergic response caused by basophil degranulation |
|
Basophils |
Mediate immediate hypersensitivity rxns (type I, anaphylactic) |
|
CD2, CD3 |
Lymphoid, pan T cells CD marker |
|
CD4 |
Helper/inducer Tcells CD marker |
|
CD8 |
Suppressor/T cytotoxic CD marker |
|
CD13 |
Pan myeloid cd marker |
|
CD11c, CD14 |
Monocytes CD marker |
|
CD19, CD20 |
Lymphoid, pan B cells CD marker |
|
CD 33 |
Pan myeloid cell CD marker |
|
CD34 |
Stem cell maker |
|
CD16, CD56 |
NK cells CD marker |
|
Myeloblast |
Earliest recognizable granulocyte precursor |
|
Promyelocyte |
Primary granules |
|
Myeloperoxidase (azurophilic granules) |
What is contained in 1° granules |
|
Myelocyte |
First stage where granulocyte types can be differentiated into eos, baso and neu |
|
Myelocyte |
Has 2° granules; last stage capable of mitosis |
|
Alkaline phosphatase, lysozyme, lactoferrin |
Contained in 2° granules |
|
Myelocyte |
Has prominent golgi apparatus |
|
Metamyelocyte |
Juvenile cell |
|
<1/2 |
Nuclear indent in metamyelocyte |
|
>1/2 |
Nuclear indent of band neutrophil |
|
Necrosis |
Induced by extracellular forces such as lethal chemical, biological and physical events Blood cell is killed |
|
Apoptosis |
Programmed cell death |
|
Dohle bodies |
Small oval inclusions in cytoplasm stains light blue |
|
Regenerative shift to the left |
Most common type of left shift |
|
Leukemoid |
High LAP |
|
CML |
Low LAP Philadelphia |
|
Pathologic neutrophilia |
With shift to the left Response to tissue damage |
|
Leukoerythroblastic reaction |
Immature leukocytes and immature erythrocytes in blood |
|
Indirectly proportional |
Eo and ACTH |
|
Chronic granulomatous disease |
Males to females 6:1 Oxidative respiratory pathway defect Inability to degranulate and bactericidal function Sex linked and autosomal |
|
Chediak-Higashi syndrome |
Giant cytoplasmic granules Abnormal fusion of 1° and 2° neutrophilic granules autosomal recessive |
|
Pelger huet anomaly |
Autosomal dominant Pince-nez Nucleus dumbbell or peanut shaped |
|
May-Hegglin Anomaly |
Autosomal dominant Dohle-like inclusion Gray blue and cigar shaped Giant plt |
|
Alder-reilly anomaly |
Autosomal recessive Azurophilic granules |
|
Kupffer cells |
Monocytes in liver |
|
Microglial cells |
Monocytes in CNS |
|
Osteoclasts |
Monocyte in bones |
|
Langerhan's cell |
Monocyte in skin |
|
Alveolar cells |
Monocytes in lungs |