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4 Cards in this Set
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C3 nephritic factor |
Add C3b and Factor B to Elisa plate —> C3 convertase Add serum Add anti human IgG |
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Fluid phase C3 |
Add patient serum to healthy control serum. Do WB to detect C3. Compare with normal control serum to work out whether more C3 break down or not. |
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HA |
Sheep RBCs incubated with mix of health and patient serum. Work out percent lysis compared to 0% lysis and 100% lysis |
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How does opsonophagocytosis assay work? |
Add patient cells to staph with patient sera and control sera Add control cells to staph w patient sera and control sera If phagocytosis occurs, oxidising ions are produced which with luminol compound that has been added and diffuses into cells —> produces light |
