Case Study 1:
1.The most likely cause of the ABO typing discrepancy in the reverse type is due to “unexpected antibodies” such as a cold/room temperature reacting alloantibody.
2.Since the auto control didn’t agglutinate, an alloantibody is suspected. With that being said, it needs to be one that reacts at room temperature. Furthermore, based on the antigen typing of the patient P1 is suspected to be interfering with the ABO results. The Lewis antigen was ruled out due to it being present on the patient’s RBCs. Moreover, from the panel results (Jsa), Fya, (S) are also unexpected antibodies that need to be considered when transfusing the patient.
3.To further confirm the presence or absence of the P1 antibody is interfering with ABO typing, …show more content…
If more blood were needed the patient would need blood that is B positive, P1 negative, and Fya negative.
Case Study 2:
1.The patient is a candidate for Rh Immune Globulin since she is at risk of forming antibodies due to her pregnancy since she is Rh negative while her baby is positive for the Rh factor. She need to take medication, RhoGAM, in order to prevent HDN caused by the Rh factor.
2.A positive DAT means there are antibodies attached to the RBCs. If the antibodies attached are part of the Rh factor the baby is at risk of anemia and HDN. However, the eluate of the baby needs to be tested to detect and identify the antibody attached to the baby’s RBCs.
3.Based on the panel results from Baby L, no unexpected antibodies are found especially for the Rh factor, however there were positive results for A1 and B for reverse grouping of cells. This means A,B is the antibody detected to be on the baby’s …show more content…
The positive DAT is owing to IgG antibodies are present on the patient’s RBCs. Although the patient has a history of taking medication and the drug interactions are what could be causing these positive results. Medications sometimes induce the formation of antibodies and may cause autoimmune mechanisms similar to autoimmune disease. These results seem to have come out positive due to a drug-independent mechanism.
2.The patient’s most probable genotype is Ce/ce which is r’r. The patient is D negative, C positive, E negative, c positive and e positive for their phenotype.
3.There is a change in the Rh typings between the slide/tube reagents and the chemically modified and saline reagents because the slide/tube have higher proteins involved compared to the others, as well as this slide/tube still have the IgG attached to the patient’s RBCs. The chemically modified and the saline reagents had the RBCs adsorbed to remove the attached antibodies in order to remove the drug-independent mechanisms attached to the RBCs that were obscuring the antibody screen results.
4.A warm autoadsorption helps as the patient wasn’t recently transfused and the patient red cells are used to remove warm autoantibodies to determine if antibodies are
1.The most likely cause of the ABO typing discrepancy in the reverse type is due to “unexpected antibodies” such as a cold/room temperature reacting alloantibody.
2.Since the auto control didn’t agglutinate, an alloantibody is suspected. With that being said, it needs to be one that reacts at room temperature. Furthermore, based on the antigen typing of the patient P1 is suspected to be interfering with the ABO results. The Lewis antigen was ruled out due to it being present on the patient’s RBCs. Moreover, from the panel results (Jsa), Fya, (S) are also unexpected antibodies that need to be considered when transfusing the patient.
3.To further confirm the presence or absence of the P1 antibody is interfering with ABO typing, …show more content…
If more blood were needed the patient would need blood that is B positive, P1 negative, and Fya negative.
Case Study 2:
1.The patient is a candidate for Rh Immune Globulin since she is at risk of forming antibodies due to her pregnancy since she is Rh negative while her baby is positive for the Rh factor. She need to take medication, RhoGAM, in order to prevent HDN caused by the Rh factor.
2.A positive DAT means there are antibodies attached to the RBCs. If the antibodies attached are part of the Rh factor the baby is at risk of anemia and HDN. However, the eluate of the baby needs to be tested to detect and identify the antibody attached to the baby’s RBCs.
3.Based on the panel results from Baby L, no unexpected antibodies are found especially for the Rh factor, however there were positive results for A1 and B for reverse grouping of cells. This means A,B is the antibody detected to be on the baby’s …show more content…
The positive DAT is owing to IgG antibodies are present on the patient’s RBCs. Although the patient has a history of taking medication and the drug interactions are what could be causing these positive results. Medications sometimes induce the formation of antibodies and may cause autoimmune mechanisms similar to autoimmune disease. These results seem to have come out positive due to a drug-independent mechanism.
2.The patient’s most probable genotype is Ce/ce which is r’r. The patient is D negative, C positive, E negative, c positive and e positive for their phenotype.
3.There is a change in the Rh typings between the slide/tube reagents and the chemically modified and saline reagents because the slide/tube have higher proteins involved compared to the others, as well as this slide/tube still have the IgG attached to the patient’s RBCs. The chemically modified and the saline reagents had the RBCs adsorbed to remove the attached antibodies in order to remove the drug-independent mechanisms attached to the RBCs that were obscuring the antibody screen results.
4.A warm autoadsorption helps as the patient wasn’t recently transfused and the patient red cells are used to remove warm autoantibodies to determine if antibodies are