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23 Cards in this Set
- Front
- Back
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where is glucose higher: venous or capillay blood?
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capillary
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oral glucose tolerance pregnancy?
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load 100 grams glucose
diagnosed if fasting > 105 mg/dL 1 hr > 190 mg/dL 2 hr > 165 3 hr > 145 |
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most specific glucose determination enzymatic method
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hexokinase method
(hexokinase catalyzes phosophorylatoin of glucose to G-6-PO4 in presence of NADP) |
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serum should be removed from cells to prevent decrease in glucose level due to glycolysis within....
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1 hour
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what specimen needed for Hemoglobin A1c
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whole blood
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most common method for HbA1c
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affinity chromoatography
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when are ketones present in blood
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breakdown of fatty acids:
- uncontrolled diabetes - starvation - vomiting - low carbohydrate diet |
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reagent used to detect ketones
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Na Nitroprusside
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if blood drawn for cholesterol right away (without pt. steated at least 5 minutes) cholesterol level will be...
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higher
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most common method for cholesterol determination
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cholesterol esterase
and cholesterol oxidase |
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friedwald formula for LDL cholesterol
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LDL cholesterol = Total cholest. - HDL cholest - Triglycerides/5
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largest of lipoproteins?
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chylomicrons
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if nonfasting specimen which lipids will be useful?
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total cholesterol
HDL cholesterol if cholest > 200 mg/dL or HDL < 40 mg/dL fasting specimen needed |
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cream layer on top of plasma after refrigeration
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high level trriglycerides or chylomicrons
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L/S ratio in amniotic fluid > 2.0 indicates...
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mature fetal lung
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most common method for serum proteins
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biuret method (cuprous ions react w/ peptide bonds at alkaline pH to produce colored complex)
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best method for CSF proteins
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trichloracetic acid (precipitates urine in CSF or urine)
biuret method is not sensitive enough |
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if hemolysis the serum protein level will be....
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increased
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dyes for direct analysis of albumin
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bromcresol green or purple (BCG or BCP)
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normal A:G ratio
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1 - 1.8
reversed in monoclonal gammopathies or when globulin increases |
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in the body what charge do most proteins carry
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at pH 7.4 proteins anre negatively charged (anions)
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support media for electrophoresis that
separate proteins by molecular charge |
cellulose acetate or agarose
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support media for electrophoresis that
separate proteins by both charge and size |
starhc and polyacrylamide gel
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