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61 Cards in this Set
- Front
- Back
- 3rd side (hint)
ANALYTICAL REAGENT |
Labels on these reagents either state the actual impurities for each chemical lot or the list maximum allowable impurities |
Labels either have the initials "AR" or "ACS" |
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ULTRAPURE |
Use in specific procedures such as chromatography, AAS, Fluorometry or other techniques that require pure chemicals |
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CHEMICALLY PURE |
Preparation is not uniform. Not recommended for clinical laboratories |
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US PHARMACOPEIA and NATIONAL FORMULARY |
Use of manufacture drugs |
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TECHNICAL/COMMERCIAL GRADE |
Used primarily in manufacturing and should never be used in the clinical laboratory |
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Distilled Water |
Purified by distillation, purified to remove almost all organic materials |
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Deionized Water |
Water purified by ion exchange |
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Reverse-Osmosis |
Water purified by reverse osmosis |
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Type 1 |
Used in clinical chemistry. Has the highest purity |
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Type 2 |
Acceptable for most analytical measurements/tests |
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Type 3 |
Autoclave/wash water; for glassware washing but not for analysis or reagent prep. |
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Polystyrene |
Plastic Ware: Rigid clear, should not be autoclaved, used for test tubes and grad. cylinder |
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Polyethylene |
Plastic Ware: Relatively inert chemically, resistant to most acids, should not be autoclaved |
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Polypropyline |
Plastic Ware: Relatively inert chemically, resistant to most acids, can be autoclaved |
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Teflon |
Plastic Ware: Resistant to wide range of Temperature |
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Polycarbonate |
Plastic Ware: Very clear and ideal for grad. cylinder |
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Polyvinylchloride |
Plastic Ware: Soft and flexible materials used frequently to construct tubing |
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Polyolefin |
Plastic Ware: Noted for their strength and resistance to elevated temp. |
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High Thermal Borosilicate |
Types of Glass: High resistance to thermal shock and chemical attacks, can be autoclaved and heated |
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High Silica |
Types of Glass: Acid and alkali resistant, heat and electrical tolerance. Used for high precision analytic work and optical reflectors and mirrors |
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Low Actinic |
Types of Glass: Red or Amber colored to prevent exposure of photosensitive light |
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Flint Glass |
Types of Glass: Used for disposable glasswares |
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Aluminosilicate Glass |
Types of Glass: 6 times stronger than borosilicate |
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High Thermal |
Types of Glass: Resist high temp only |
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Boron Free |
Types of Glass: Used for highly alkaline sol'n, alkali resistant, poor heat resistance |
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Volumetric Flask |
Laboratory Vessel: Has round flat lower portion and a long, thin neck. Calibrated to hold 1 exact volume |
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Erlenmeyer Flask |
Laboratory Vessel: Has a wide bottom that gradually evolves into a smaller, short neck |
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Griffin Beaker |
Laboratory Vessel: Has a flat bottom, traight sides with a small spout in the lip |
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Graduated Cylinder |
Laboratory Vessel: Long cylindrical usually held upright by an octagonal or circular base |
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are you ready? |
PIPETTES |
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To Contain |
TC or TD: holds a particular volume but does not dispense that exact volume, delivers the amount by washing it and emptying |
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To Deliver |
TC or TD: will dispense the volume indicated, transfer exact amount, do not blow the last drop, rate of flow is gravity |
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Blowout |
w/ etched/colored ring near the mouth A. Blowout B. Self draining |
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Self Draining |
Markings are absent A. Blow-out B. Self-draining |
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Measuring or Graduated |
capable of dispensing several different volumes |
Serologic, Mohr, Micropipette |
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Transfer |
designed to dispense one volume without further subdivisions |
Pasteur, Volumetric Pipette, Ostwald folin, Automatic Pipette |
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Air Displacement |
Tips can only be used once, piston mechanism, air movement |
A. Air Displacement B. Positive Displacement C. Dispenser Pipets |
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Dispenser Pipets |
Obtain from a common reservoir and dispense it repeatedly |
A. Air DisplacementB. Positive DisplacementC. Dispenser Pipets |
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Ready? Go! |
SEPARATION TECHNIQUES |
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Centrifugation |
A process whereby centrifugal force is used to separate solid matter from a liquid suspension |
A. Filtration B. Dialysis C. Centrifugation |
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Dialysis |
Method for separating macromolecules from a solvent or smaller substances |
A. FiltrationB. DialysisC. Centrifugation |
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Filtration |
Used in place of centrifugation |
A. FiltrationB. DialysisC. Centrifugation |
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Fixed Angle Centrifuge |
One Angle (25-40°); 7000 rpm |
A. Ref. Centri B. Swinging Bucket C. Cytocentri D. Fixed Angle centri |
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Swinging bucket centri |
When at rest, tubes are in vertical position During centri, tubes are in horizontal position |
A. Ref. CentriB. Swinging BucketC. CytocentriD. Fixed Angle centri |
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Ref. Centri/Ultracentri |
Used for thermally labile test analytes |
A. Ref. CentriB. Swinging BucketC. CytocentriD. Fixed Angle centri |
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RCF (Relative Centrifugal Force) |
Force acting in the sample |
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RPM (Revolutions Per Minute) |
Actual speed of the centrifuge |
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Tachometer |
Used to calibrate the speed of centrifuge |
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Concentrated |
Large quantity of solute in solution |
A. Supersaturated B. Saturated C. Concentrated D. Dilute |
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Dilute |
One in which there is relatively little solute |
A. SupersaturatedB. SaturatedC. ConcentratedD. Dilute |
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Supersaturated |
Greater concentration of undissolved solute particles. Thermodynamically unstable |
A. SupersaturatedB. SaturatedC. ConcentratedD. Dilute |
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Saturated |
Sol'n in which there is an excess of undissolved solute particles |
A. SupersaturatedB. SaturatedC. ConcentratedD. Dilute |
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Boiling Point |
Temp at which vapor pressure of the solvent reaches 1 atm |
A. Vapor Pressure B. Freezing Point C. Boiling Point D. Osmotic Pressure |
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Vapor Pressure |
Pressure at which the liquid solvent is in equilibrium with the water vapor |
A. Vapor PressureB. Freezing PointC. Boiling PointD. Osmotic Pressure |
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Osmotic Pressure |
Pressure that allows solvent flow through a semipermeable membrane to establish equilibrium bet. compartments of different conc. |
A. Vapor PressureB. Freezing PointC. Boiling PointD. Osmotic Pressure |
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Freezing Point |
Temp. at which the vapor pressure of the solid and liquid phases are the same |
A. Vapor PressureB. Freezing PointC. Boiling PointD. Osmotic Pressure |
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Beer's Law |
Absorption is directly proportional to Conc. and inversely proportional to %T |
A. Charle's Law B. Dalton's Law C. Beer's Law |
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Boyle's Law |
Volume of Gas and Pressure |
A. Charle's Law B. Boyle's Law C. Avogadro's Principle |
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Charle's Law |
Volume of Gas and Temperature |
A. Charle's Law B. Beer's Law C. Dalton's Law |
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Avogadro's Principle |
Gram molecular weight and # of atoms |
A. Charle's Law B. Boyle's Law C. Avogadro's Principle |
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Dalton's Law |
Volume of one Gas to another |
A. Charle's LawB. Beer's LawC. Dalton's Law |