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10 Cards in this Set
- Front
- Back
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Which formula correctly describes the relationship between absorbance and %T ? A. A = 2 – log %T B. A = log 1/T C. A = –log T D. All of these options |
D. All of these options |
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A solution that has a transmittance of 1.0 %T would have an absorbance of: A. 1.0 B. 2.0 C. 1% D. 99% |
B. 2.0 |
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In absorption spectrophotometry: A. Absorbance is directly proportional to transmittance B. Percent transmittance is directly proportional to concentration C. Percent transmittance is directly proportional to the light path length D. Absorbance is directly proportional to concentration |
D. Absorbance is directly proportional to concentration |
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Which wavelength would be absorbed strongly by a red-colored solution?
A. 450 nm B. 585 nm C. 600 nm D. 650 nm |
A. 450 nm |
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A green-colored solution would show highest transmittance at: A. 475 nm B. 525 nm C. 585 nm D. 620 nm |
B. 525 nm |
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SITUATION: A technologist is performing an enzyme assay at 340 nm using a visible-range spectrophotometer. After setting the wavelength and adjusting the readout to zero %T with the light path blocked, a cuvette with deionized water is inserted. With the light path fully open and the 100%T control at maximum, the instrument readout will not rise above 90%T. What is the most appropriate first course of action? A. Replace the source lamp B. Insert a wider cuvette into the light path C. Measure the voltage across the lamp terminals D. Replace the instrument fuse |
A. Replace the source lamp |
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Which type of monochromator produces the purest monochromatic light in the UV range? A. A diffraction grating and a fixed exit slit B. A sharp cutoff filter and a variable exit slit C. Interference filters and a variable exit slit D. A prism and a variable exit slit |
D. A prism and a variable exit slit |
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Which monochromator specification is required in order to measure the true absorbance of a compound having a natural absorption bandwidth of 30 nm? A. 50-nm bandpass B. 25-nm bandpass C. 15-nm bandpass D. 5-nm bandpass |
D. 5-nm bandpass |
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Which photodetector is most sensitive to low levels of light? A. Barrier layer cell B. Photodiode C. Diode array D. Photomultiplier tube |
D. Photomultiplier tube |
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Which condition is a common cause of stray light? A. Unstable source lamp voltage B. Improper wavelength calibration C. Dispersion from second-order spectra D. Misaligned source lamp |
C. Dispersion from second-order spectra |
