Ch 20 Part 3

Front 1

As genetic technology makes testing for a wide variety of genotypes possible, which of the following is likely to be an increasingly troublesome issue?
A) use of genotype information to provide positive identification of criminals
B) using technology to identify genes that cause criminal behaviors
C) the need to legislate for the protection of the privacy of genetic information
D) discrimination against certain racial groups because of major genetic differences
E) alteration of human phenotypes to prevent early disease

Back 1

the need to legislate for the protection of the privacy of genetic information

Front 2

Which enzyme was used to produce the molecule in Figure 20.1?
A) ligase
B) transcriptase
C) a restriction enzyme
D) RNA polymerase
E) DNA polymerase

Back 2

a restriction enzyme

Front 3

The segment of DNA shown in Figure 20.2 has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments?
A)

B)

C)

D)

E)

Back 3

B

Front 4

Which of the following statements is consistent with the results?
A) B is the child of A and C.
B) C is the child of A and B.
C) D is the child of B and C.
D) A is the child of B and C.
E) A is the child of C and D.

Back 4

C is the child of A and B.

Front 5

Which of the following statements is most likely true?
A) D is the child of A and C.
B) D is the child of A and B.
C) D is the child of B and C.
D) A is the child of C and D.
E) B is the child of A and C.

Back 5

D is the child of A and B.

Front 6

Which of the following are probably siblings?
A) A and B
B) A and C
C) A and D
D) C and D
E) B and D

Back 6

C and D

Front 7

) Bacteria that contain the plasmid, but not the eukaryotic gene, would grow
A) in the nutrient broth plus ampicillin, but not in the broth containing tetracycline.
B) only in the broth containing both antibiotics.
C) in the broth containing tetracycline, but not in the broth containing ampicillin.
D) in all four types of broth.
E) in the nutrient broth without antibiotics only.

Back 7

in all four types of broth.

Front 8

Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow in
A) the nutrient broth only.
B) the nutrient broth and the tetracycline broth only.
C) the nutrient broth, the ampicillin broth, and the tetracycline broth.
D) all four types of broth.
E) the ampicillin broth and the nutrie broth.

Back 8

the ampicillin broth and the nutrie broth.

Front 9

Bacteria that do not take up any plasmids would grow on which media?
A) the nutrient broth only
B) the nutrient broth and the tetracycline broth
C) the nutrient broth and the ampicillin broth
D) the tetracycline broth and the ampicillin broth
E) all three broths

Back 9

the nutrient broth only

Front 10

Why might they be conducting such an experiment?
A) to find the location of this gene in the human genome
B) to prepare to isolate the chromosome on which the gene of interest is found
C) to find which of the students has which alleles
D) to collect population data that can be used to assess natural selection
E) to collect population data that can be used to study genetic drift

Back 10

to find which of the students has which alleles

Front 11

Their next two steps, in order, should be
A) use of a fluorescent probe for the gene sequence, then electrophoresis.
B) electrophoresis of the fragments followed by autoradiography.
C) electrophoresis of the fragments, followed by the use of a probe.
D) use of a ligase that will anneal the pieces, followed by Southern blotting.
E) use of reverse transcriptase to make cDNA, followed by electrophoresis.

Back 11

electrophoresis of the fragments, followed by the use of a probe.

Front 12

Analysis of the data obtained shows that two students each have two fragments, two students each have three fragments, and two students each have one only. What does this demonstrate?
A) Each pair of students has a different gene for this function.
B) The two students who have two fragments have one restriction site in this region.
C) The two students who have two fragments have two restriction sites within this gene.
D) The students with three fragments are said to have "fragile sites."
E) Each of these students is heterozygous for this gene.

Back 12

The two students who have two fragments have one restriction site in this region.

Front 13

Which of the following would be a reasonably efficient technique for confirming the diagnosis of CML?
A) searching for the number of telomeric sequences on chromosome 22
B) looking for a Ph' chromosome in a peripheral blood smear
C) enzyme assay for thymine kinase activity
D) FISH study to determine the chromosomal location of all chromosome 22 fragments
E) identification of the disease phenotype in review of the patient's records

Back 13

FISH study to determine the chromosomal location of all chromosome 22 fragments

Front 14

Why would Gleevec most probably cause remission of the disease?
A) It reverses the chromosomal translocation.
B) It eliminates the Ph' chromosome.
C) It removes Ph'-containing progenitor cells.
D) The drug inhibits the replication of the affected chromosome.
E) The drug inhibits the specific thymine kinase receptor.

Back 14

The drug inhibits the specific thymine kinase receptor.

Front 15

One possible use of transgenic plants is in the production of human proteins, such as vaccines. Which of the following is a possible hindrance that must be overcome?
A) prevention of transmission of plant allergens to the vaccine recipients
B) prevention of vaccine-containing plants being consumed by insects
C) use of plant cells to translate non-plant-derived mRNA
D) inability of the human digestive system to accept plant-derived protein
E) the need to cook all such plants before consuming them

Back 15

prevention of transmission of plant allergens to the vaccine recipients

Front 16

Patients whose tumors are HER-2 positive respond to herceptin whereas other patients do not. Patients whose tumors have estrogen receptors will be best served if
A) their estrogen receptors are blocked by using RNAi.
B) their estrogen release is activated and/or elevated.
C) the estrogen receptors are blocked by other molecules that can use the same receptors.
D) they are given herceptin as well as estrogen.
E) they are given a complete hysterectomy.

Back 16

the estrogen receptors are blocked by other molecules that can use the same receptors.

Front 17

Breast tumor biopsy specimens can be typed for a number of gene expression patterns. Together, these can provide risk analysis for the likely aggressive growth and metastasis of the tumor. How can this most help the physician and patient?
A) Some patients want to know as much as possible.
B) This can help them to decide whether and what kind of chemotherapy is warranted.
C) This can help them decide whether the tumor should be removed.
D) Some physicians may use the information to decide what to do, but not tell the patient.
E) This can help to aggregate health statistics.

Back 17

This can help them to decide whether and what kind of chemotherapy is warranted.

Front 18

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?
A) restriction enzymeanalysis of RFLPs
B) DNA ligasecutting DNA, creating sticky ends of restriction fragments
C) DNA polymerasepolymerase chain reaction to amplify sections of DNA
D) reverse transcriptaseproduction of cDNA from mRNA
E) electrophoresisseparation of DNA fragments

Back 18

DNA ligasecutting DNA, creating sticky ends of restriction fragments

Front 19

Plants are more readily manipulated by genetic engineering than are animals because
A) plant genes do not contain introns.
B) more vectors are available for transferring recombinant DNA into plant cells.
C) a somatic plant cell can often give rise to a complete plant.
D) genes can be inserted into plant cells by microinjection.
E) plant cells have larger nuclei.

Back 19

a somatic plant cell can often give rise to a complete plant.

Front 20

A paleontologist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird). To compare a specific region of the DNA from the sample with DNA from living birds, which of the following would be most useful for increasing the amount of dodo DNA available for testing?
A) RFLP analysis
B) polymerase chain reaction (PCR)
C) electroporation
D) gel electrophoresis
E) Southern blotting

Back 20

polymerase chain reaction (PCR)

Front 21

DNA technology has many medical applications. Which of the following is not done routinely at present?
A) production of hormones for treating diabetes and dwarfism
B) production of microbes that can metabolize toxins
C) introduction of genetically engineered genes into human gametes
D) prenatal identification of genetic disease alleles
E) genetic testing for carriers of harmful alleles

Back 21

introduction of genetically engineered genes into human gametes

Front 22

In recombinant DNA methods, the term vector can refer to
A) the enzyme that cuts DNA into restriction fragments.
B) the sticky end of a DNA fragment.
C) a SNP marker.
D) a plasmid used to transfer DNA into a living cell.
E) a DNA probe used to identify a particular gene.

Back 22

a plasmid used to transfer DNA into a living cell.

Front 23

Which of the following would not be true of cDNA produced using human brain tissue as the starting material?
A) It could be amplified by the polymerase chain reaction.
B) It could be used to create a complete genomic library.
C) It was produced from mRNA using reverse transcriptase.
D) It could be used as a probe to detect genes expressed in the brain.
E) It lacks the introns of the human genes.

Back 23

It could be used to create a complete genomic library.

Front 24

Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of
A) post-transcriptional processing.
B) electroporation.
C) post-translational processing.
D) nucleic acid hybridization.
E) restriction fragment ligation.

Back 24

post-transcriptional processing.

Front 25

Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme?
A) AAGG
TTCC

B) AGTC
TCAG

C) GGCC
CCGG

D) ACCA
TGGT

E) AAAA
TTTT

Back 25

GGCC
CCGG