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7 Cards in this Set
- Front
- Back
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What is the purpose of making a reserve stock of your unknown?
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The reserve stock serves as a backup in case the working stock culture becomes contaminated.
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After preparing working and reserve stock cultures, what is the next critical step in identifying your unknown bacterium?
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gram stain
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After performing a gram stain on your unknown bacterium, when is it appropriate to also perform a spore stain?
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when organism is a gram-positive rod
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After performing a gram stain on your unknown bacterium, when is it appropriate to also perform an acid-fast stain
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when organism is a gram-positive rod
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What type of medium can be inoculated to study motility of your unknown?
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semisolid or SIM
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What is the purpose of an EMB plate? 2
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The dyes (Eosine and Methylene Blue) inhibit the growth of Gram-positive bacteria
The medium contains amino acids and small peptides that support the growth of all Gram-negative rods |
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What is the purpose of an PEA plate?
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prevents the growth of Gram-negative organisms by disrupting the sturcture of lipids in the Gram-negative membrane.
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