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10 Cards in this Set
- Front
- Back
In the Schaeffer-Fulton spore stain, which dye is used to stain the endospore?
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Malachite green
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In the Schaeffer-Fulton spore stain, which stain is used to stain the sporangium?
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Safranin
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Name the two bacterial genera that form endospores.
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Bacillus and Clostridia
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Describe evolutionary advantages associated with formation of an endospore. 2
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Allows a cell to survive harsh environmental conditions and become resistant to heat, radiation, and/or chemicals.
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What is the name of the protective coat that surrounds an endospore?
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Exosporium
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What type of biological molecule makes up the exosporium?
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protein
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In a Schaeffer-Fulton spore stain, what must you do in order to get the primary stain to penetrate the endospore?
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Heat the slide while applying stain
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What is the heat resistance of enspores dependent on?
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content of water
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How can endospores be destroyed by heat?
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be exposed to heat 15-20 minutes with steam under pressure (autoclave)
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How does the slide look when a Dorner Method is done?
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red spores
colorless sporangium |