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22 Cards in this Set
- Front
- Back
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Name the two multiple test media which can be used to test for production of hydrogen sulfide.
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Kligler’s iron agar
SIM |
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What does Kligker's iron agar predict? 3
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fermemtation of lactose
fermentation of glucosr production of hydrogen sulfide |
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What does SIM test predict?
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production of indole
motility production of hydrogen sulfide |
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What does a positive hydrogen sulfide test look like?
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black precipitate
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Hydrogen sulfide is one of the products formed from the degradation of ____________________. The enzyme involved in this reaction is __________________.
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cysteine
cysteine desulfurase |
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What reagent is necessary to detect the presence of indole?
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Kovacs’ (reagent)
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Litmus is used to test for changes in what property?
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pH
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Skim milk contains what type of sugar?
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lactose
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What are the proteins found in skim milk? 3
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casein
lactalbumin lactogloblin |
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Digestion of milk proteins involves enzymes called ________________________.
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proteases
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Describe the chemical reaction which causes litmus to turn pink. 2
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fermentation of lactose
production of acid |
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Describe the chemical reaction which causes litmus to turn purple or blue.
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proteolysis
production of ammonia |
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What indicator is used i Kliger's iron agar?
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phenol red
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KIA: red slant & yellow butt?
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glucose was utilized
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KIA: yellow slant & yellow butt?
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fermented both lactose & glucose
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KIA: red slant & red butt?
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no fermentation of glucose or lactose
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Indicator used in Litmus milk?
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litmus
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Litmus milk: purple?
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release of ammonia (alkaline)
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Litmus milk: Pink?
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ferments milk sugar (acidic)
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Limus Milk: white?
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litmus milk dye is reduced to a drop in oxygen levels in the culture
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Litmus milk: curd formation?
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coagulation from precipitation and coagulation of proteins
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Litmus milk: translucent?
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peptonization
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