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11 Cards in this Set

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Write out the reactions of the glycolytic pathway from glucose to lactate using structural formulas for all intermediates. Learn the names of these intermediates and the enzymes that catalyze the reactions.
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Iodoacetic acid inhibits glycolysis by inactivating glyceraldehyde-3-P dehydrogenase. Fructose 1,6 bis-P accumulates under these conditions. Why does this metabolite accumulate rather than glyceraldehyde-3-P and dihydroxyacetone phosphate, the products immediately preceding GAPDH?
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In which carbon atoms of pyruvate would radioactivity be found if glucose metabolized by the glycolytic pathway were labeled with 14C at (a) C-1 and (b) C-4?
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Fluoride inhibits enolase by reaction with Mg+2 to form the precipitate, MgFPO3. What metabolite intermediate will accumulate when F is added to cell-free extracts capable of glycolysis?
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If [1-13C]-glucose is the substrate for glycolysis in yeast cells the labelled 13C soon appears in C-1 and C-6 of fructose-1,6-bis-P. Explain how this scrambling occurs.
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Describe the reaction mechanisms of aldolase (Schiff base formation, electron sink), triose-P isomerase or P-glucose-isomerase (enediol intermediate) and glyceraldehyde 3-P dehydrogenase (thioester formation, covalent enzyme intermediate in energy coupling).
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In reactions not requiring structures, describe the reactions that allow galactose, fructose and mannose carbons to be metabolized via glycolysis. Specify the organs where these reactions are prevalent.
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Describe 2 anaerobic catabolic fates of pyruvate?
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Why is the the cell's ability to regenerate NAD+ critical to glycolysis?
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What is the net yield of ATP per glucose molecule by the glycolytic pathway?
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Explain what is meant by the preparatory phase and payoff phases of glycoysis?
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