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5 Cards in this Set
- Front
- Back
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Gram stain
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thick peptidoglycan is +. CIAS
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Technique for pure culture isolation
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4-O- Clock single colony isolation
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Selective Media
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Pheny/Ethyl/Alcohol (PEA) agar: inhibits gram neg, promotes gram +
Crystal violet agar: inhibits gram + and promotes gram - 7.5 % NaCl agar: selects halophilic like Staph bacteria. |
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Differential Media
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Many grow but shows different characteristics.
Mannitol NaCl agar: staph that ferments mannitol; acid turns the phenol red into yellow. MacConkey aga: cristal violet and bile inhibit gram +, lactose ad neutral red detect lactose fermentation. EMB Eosin. Methylene inhibits gram +, eosin goes black in lactose fermentation. Coli acid ferment produces metallic green. |
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Enriched media
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For fastidious species
Blood sheep agar: Gamma hemol-media is red not hemol Alpha: partial lysis media green Beta: complete lysis, clearings |
