Figure 1: Pepperwood Preserve study system for U. californica for susceptibility of P. ramorum depicted by climate and topography aerial map. No image rights are owned and the map was created by Celeste Dodge. Two red boxes in bottom right of the image indicates the approximate location of the two study sites.
In October of 2016, bay leaves were collected at Pepperwood Preserve, Sonoma County at a study system composed of mixed evergreen forest that interfaces with nearby serpentine grasslands and oak woodlands. The site has a south-west aspect that receives evening sunlight, and has varying degrees of slope. Branches were collected at varying degrees of reachable height below below 3-meters and represent a limited aspect of U. californica …show more content…
ramorum susceptibility, plastic tubs were filled with 500 mL of vermiculite to maintain a representative humidity. Twelve leaves of randomly were placed flat in the tubs and treatment was induced on their underside of either zoospore or water, with an average of 10 drops being placed per leaf. Leaves labeled for zoospore inoculation were treated with 10 drops of zoospores (10ul) and those labeled for control were treated with 10 drops of sterile soil water (10uL) using proper pipette technique. Tubs were then sealed, stored and covered in the dark for 7 days at approximately 25°C. Data measurements were conducted by hand, and included the frequency of necrosis as a measure of number of potential infections and the magnitude (size) of necrosis …show more content…
californica-P. ramorum pathosystem should utilize microscopy to progress the observable record of which physical mechanisms are involved in the infection and spread of necrosis, such as structural elements like the stomata and waxy cuticle and how they are functioning. If specific molecules or macromolecules were detected or suspected of influencing susceptibility, then follow-up radio-active isotopes testing could be used to analyze quantities, concentration and transport of such a substance during pathosystem interactions. To further understand the implications of moisture dynamics on P. ramorum, climate data should be coupled with future research, especially with climate change is having greater influences on field data analysis. In conclusion, U. californica leaves had an increased susceptibility to P. ramorum after their first year of development, while the water content potential within the leaf had no influence on the occurrence or magnitude of necrosis. Our results strongly suggest that bay leaves undergo a physiological change during leaf development which decreases their resistance to P.
In October of 2016, bay leaves were collected at Pepperwood Preserve, Sonoma County at a study system composed of mixed evergreen forest that interfaces with nearby serpentine grasslands and oak woodlands. The site has a south-west aspect that receives evening sunlight, and has varying degrees of slope. Branches were collected at varying degrees of reachable height below below 3-meters and represent a limited aspect of U. californica …show more content…
ramorum susceptibility, plastic tubs were filled with 500 mL of vermiculite to maintain a representative humidity. Twelve leaves of randomly were placed flat in the tubs and treatment was induced on their underside of either zoospore or water, with an average of 10 drops being placed per leaf. Leaves labeled for zoospore inoculation were treated with 10 drops of zoospores (10ul) and those labeled for control were treated with 10 drops of sterile soil water (10uL) using proper pipette technique. Tubs were then sealed, stored and covered in the dark for 7 days at approximately 25°C. Data measurements were conducted by hand, and included the frequency of necrosis as a measure of number of potential infections and the magnitude (size) of necrosis …show more content…
californica-P. ramorum pathosystem should utilize microscopy to progress the observable record of which physical mechanisms are involved in the infection and spread of necrosis, such as structural elements like the stomata and waxy cuticle and how they are functioning. If specific molecules or macromolecules were detected or suspected of influencing susceptibility, then follow-up radio-active isotopes testing could be used to analyze quantities, concentration and transport of such a substance during pathosystem interactions. To further understand the implications of moisture dynamics on P. ramorum, climate data should be coupled with future research, especially with climate change is having greater influences on field data analysis. In conclusion, U. californica leaves had an increased susceptibility to P. ramorum after their first year of development, while the water content potential within the leaf had no influence on the occurrence or magnitude of necrosis. Our results strongly suggest that bay leaves undergo a physiological change during leaf development which decreases their resistance to P.