Virology Exam 2

Front 1

What structure(s) is (are) found in the poxvirus?

Back 1

lateral bodies

surface tubules

palisade layer

core fibrils

Front 2

Herpesvirus latency: occurs in infected cells where ____________ and can be ________________.

Back 2

1) multiplication cycle is stopped

2) lysogenic

Front 3

Where does the poxvirus acquire an envelope?

Back 3

Golgi apparatus

Front 4

What are the subfamilies of Herpesviridae?

Back 4

Alpha, Beta, Gamma

Front 5

Polyomaviruses adsorb to cell receptors similar to...

Back 5

growth factor receptors

Front 6

poxviruses transcribes ____ of their DNA as early genes (fraction)

Back 6

1/3

Front 7

Varicella-zoster more severe in adults or children

Back 7

adults

Front 8

varicella-zoster is spread by the...

Back 8

respiratory tract

Front 9

varicella zoster has seasonal ______, the infection cycle lasts ______, and its recurrent infection is called _______

Back 9

1) epidemic

2) 2-2.5 weeks

3) shingles

Front 10

Name clinical diseases of papillomavirus.

Back 10

skin warts, neoplasis of genitalia and anal region, anogenital condylomas, flat condylomas of uterine cervix

Front 11

Oral disease, pharyngitis, genital diseases, and whitlow are disease syndromes that occur with

Back 11

HSV-1 and HSV-2

Front 12

What transcript is necessary for subsequent transcription of adenovirus genome to occur?

Back 12

E1A

Front 13

Papilomaviridae DNA synthesis begins with _____ and uses a __________

Back 13

1) an origin of replication

2) an RNA primer

Front 14

Papilloma viral DNA is maintained as a ___________________ and only replicates _______________

Back 14

1) stable multicopy plasmid

2) once per cell cycle

Front 15

DNA gene E1 of papillomavirus codes for

Back 15

plasmid replication

Front 16

papillomavirus transcription uses ______-regulatory elements

Back 16

cis and trans

Front 17

Which poxvirus disease has symptoms of single/multiple benign rounded, dome-shaped, waxy papules that can be sexually transmitted and confined to the penis, pubis and inner thighs?

Back 17

Molluscum Contagiosum

Front 18

What is the main route of transmission for arbovirus infections?

Back 18

Mosquito vector (also aerosols, transplacental, tick, fecal-oral)

Front 19

What is a provirus?

Back 19

a complete DNA copy of the viral genome transmitted in the germ-line DNA from cell parent to offspring.

Front 20

How is adenovirus clinically detected?

Back 20

AD5 tranformed cells used for growing adenovirus

Feulgen stain reveals inclusions

also shows rounding and clustering of infected cells

Front 21

Keratoconjunctivitis is caused by

Back 21

Adenovirus and Herpesvirus (both!)

Front 22

For up to how long can the B19 parvovirus be contagious?

Back 22

one week

Front 23

Herpesvirus envrlopes are first acquired at the

Back 23

inner nuclear membrane

Front 24

Development of HIV-1 vaccine has been hampered because...

Back 24

many subtypes

HIV for one strain will not protect against others

high mutation rate

Front 25

Name a virus that is not pathogenic to insects

Back 25

Herpesvirus

Front 26

What virus has ds linear DNA?

Back 26

Herpesvirus

Front 27

is there a means of prevention or treatment for parvovirus?

Back 27

no

Front 28

the genome of parvoviruses contain

Back 28

inverted complementary sequences

Front 29

What is HAART?

Back 29

Combination HIV therapy involving 2 nuleoside analogs and a protease inhibitor (3 total)

Front 30

The virus that causes chicken pox is

Back 30

Varicella-zoster

Front 31

What is the hallmark feature of an HIV infection?

Back 31

Depletion of CD4+ T lymphocytes

Front 32

In adenovirus DNA replication: can replication be primed from one or both sides?

Back 32

either from one side or both sides

Front 33

in adenovirus DNA replication: what type of replication is there?

Back 33

semi-conservative

Front 34

in adenovirus DNA replication: what is used for dna priming?

Back 34

a protein

Front 35

in adenovirus DNA replication: what allows circulation of the DNA?

Back 35

terminal redundancy

Front 36

Pathogenesis of parvoviruses

Back 36

Erythema infectiosum, rash, anemia in the fetus, arthritis symptom

Front 37

components of genetic engineering of Baculovirus

Back 37

vector transfer, construction of baculovirus expression vectors, site-directed mutagenesis, determination of gene

Front 38

What are genera included in the Parvoviridae family?

Back 38

Denovirus, Parvovirus, Dependovirus, Erythrovirus

Front 39

What is the cellular tropism of Parvovirus B19?

Back 39

Erythroblasts

Front 40

Cowpox can be transmitted to humans from

Back 40

cows and cats

Front 41

up to how long does poxvirus take to complete morphogenesis?

Back 41

25 hours

Front 42

What is the approximate size of the Parvovirus?

Back 42

25nm

Front 43

What are steps in the morphogenesis of Baculovirus?

Back 43

De novo synthesis of envelope in the nucleus, cytoplasmic budding, nuclear budding, polyhedra formation

Front 44

some viruses included in the parvoviridae family integrate into chromosome...

Back 44

19

Front 45

The family poxviridae shares how many major antigens?

Back 45

1

Front 46

Poxvirus replication cycle: uncoating of cores requires the virion RNA polymerase to transcribe...

Back 46

vaccinia DNA

Front 47

poxvirus replication cycle: newly synthesized protein is rewired to uncoat...

Back 47

the virion core

Front 48

poxvirus replication cycle: cytoplasmic factories for virus multiplication are proportional to...

Back 48

MOI

Front 49

Poxvirus structure includes

Back 49

lateral bodies, spheroidin, transcriptase

(not polyhedrin)

Front 50

Herpesvirus: HSV-2 is more often associated with...

Back 50

genital infection

Front 51

Are vaccinia DNA termini covalently linked?

Back 51

yes

Front 52

are vaccinia DNA termini S or F configuration?

Back 52

both

Front 53

Do vaccinia DNA termini have tanden repeats or inverted repeats?

Back 53

both

Front 54

Name the uncoating characteristics of poxvirus:

Back 54

-uncoating of cores requires the virion DNA

-Newly synthesized protein required to uncoat virion

-1/3 DNA transcribed as early genes

-cytoplasmic factories proportional to MOI

Front 55

Polyomaviridae has circular or linear genome?

Back 55

circular (ds)

Front 56

Can herpesviruses be transmitted to children during childbirth?

Back 56

yes

Front 57

When was smallpox first eradicated worldwide?

Back 57

1977

Front 58

Baculoviruses are non-enveloped or enveloped?

Back 58

non-enveloped

Front 59

Cytopathic effects of Adenovirus?

Back 59

intranuclear inclusions, rounding and clustering cells

Front 60

adenoviruses persist in _______ for ________

Back 60

adenoids and tonsils, for years

Front 61

Adenovirus-5 can be used for

Back 61

gene therapy

Front 62

Is an adenovirus vaccine available?

Back 62

an enteric vaccine available for some subtypes, military stopped using it

Front 63

This virus is sexually transmitted and latent infection occurs by viral DNA residing in nerves of lower back.

Back 63

Herpes Simplex 2 (HSV-2)

Front 64

What is the difference between HTLV-1 and HTLV-2?

Back 64

HTLV-1 affects more CD4 cells than CD8 cells

Front 65

Polyomavirus - non-enveloped or enveloped?

Back 65

non-enveloped

Front 66

Polyomavirus: icosahedral or spherical capsid?

Back 66

icosahedral

Front 67

Polyomavirus: type of genome?

Back 67

supercoiled circular ds DNA

Front 68

Berk and sharp first identified splicing in...

Back 68

adenovirus

Front 69

What are routes of infection for the parvovirus?

Back 69

respiratory tract, blood transfusions, transplacentral (not genital)

Front 70

What are some herpesviruses?

Back 70

Roseolovirus, Epstein-Barr, Cytomegalovirus, Varicella-zoster

Front 71

What is the function of AZT in the treatment of an HIV infection?

Back 71

inhibition of viral reverse transcriptase

Front 72

Pathology of polyomavirus?

Back 72

demyelination of nerves

mild respiratory symptoms

BKV persists in urinary tract

JC virus causes professive multifocal leukoencephalopathy

Front 73

Which genes are dispensed throughout Herpesvirus genome during the multiplication cycle?

Back 73

Alpha, Beta & Gamma genes

Front 74

Which virus causes mono?

Back 74

Epstein-Barr

Front 75

What are factors in grouping adenovirus?

Back 75

Serologic

Animal

Molecular

Human

Front 76

Varicella-zosster virus is included in which subfamily?

Back 76

Alphaherpesvirinae

Front 77

DNA or portions of it are present in some tumor cells in which of the following viruses?

Back 77

HSV-2, HPV

Front 78

What are symptoms of parvovirus?

Back 78

pyrexia

headahce

biphasic rash

arthritis

Front 79

Clinical symptoms of HSV-1?

Back 79

cold sores

fever

rarely encephalitis

Front 80

Cytomegalovirus transmitted through

Back 80

milk

saliva

semen

Front 81

Diseases caused by papillomavirus include:

Back 81

anogenital condylomas

skin warts

laryngeal papillomatosis

cervical neoplasia

Front 82

An infection via parvovirus is called

Back 82

Erythema infectiosum

Front 83

Components of Adenovirus

Back 83

hexon

core protein

penton base

fiber

Front 84

Which virus is carried by 95% of people over the age of 40?

Back 84

Epstein-Barr

Front 85

The adenovirus family consists of many serotypes, and these can be distinguished from other families of viruses by

Back 85

their DNA pattern

Front 86

What virus is oncogenic?

Back 86

Papillomavirus

Front 87

What is a major component of the Baculovirus protein matrix?

Back 87

Polyhedrin

Front 88

DNA replication of the poxvirus consists of

Back 88

a nick

polymerize

flop mechanism that generates concatemers

Front 89

Symptoms of parvovirus

Back 89

fever, arthritis, itching, rash

Front 90

Retroviruses are ____ viruses and possess ______ that is responsible for the transcription of proviral DNA.

Back 90

1) positive ssRNA

2) reverse transcriptase

Front 91

E6 genes...

Back 91

transform human keratinocytes to immortality

Front 92

this serotype can persist in the tonsils and adenoids for years

Back 92

Ad1, 2, and 5

Front 93

What virus is associated an Adenovirus and helps its replication?

Back 93

Dependovirus

Front 94

In EBV infection, symptoms include pharyngitis, fever and atypical lymphocytes in...

Back 94

acute infection

Front 95

which subfamily of the herpesvirus has neurotropic tissue tropism?

Back 95

Alphaherpesvirinae

Front 96

This virus is a covalently closed dsDNA

Back 96

Baculovirus

Front 97

What is a provirus?

Back 97

a viral dsDNA that is integrated into cellular DNA

Front 98

what is the genome of parvovirus?

Back 98

linear ssDNA

Front 99

in what location does the viral DNA of HSV-1 reside during latency?

Back 99

sensory ganglia

Front 100

Clinical symptoms of adenovirus:

Back 100

pertussis-like syndrome

pharyngitis

gastroenteritis

pharyngoconjuntival fever

Front 101

This adenovirus subparticle inserts naked DNA into the host cell nucleus.

Back 101

VU3

Front 102

All of the following are used in the determination of infection virus:

Back 102

plaque assay

endpoint dilution

Bioassay

Front 103

Lymphotropic herpesviruses are...

Back 103

Gammaherpesvirinae

Front 104

In adenovirus transcription, the late genes all have ___________promoter.

Back 104

the same

Front 105

This is specific of the virion structure of only members of the Adenoviridae family.

Back 105

Fibers

Front 106

Beside adenovirus, what is the other virus that serves as a helper in the multiplication cycle of certain Parvoviridae genera?

Back 106

Herpesvirus