Rna Synthesis

Front 1

What are the steps in splicing

Back 1

1 - U1 recognizes and binds to 5' splice donor site
2-U2 binds branch site and activates A
3-U4,5,6 bind and spliceosome complete
4-phosphodiester bond b/w exon and donor G cleaved by activated A
5-5' phosphate of G forms 5'-->2' linkage with 2'OH of branch site A--> lariate
6-phosphodiester bond b/w acceptor G and next exon cleaved, lariat released, exons joined

NEEDS ATP

Front 2

What does the intron/exon unit look like?

Back 2

5' splice donor site - GU
3' splice acceptor site - AG
Branch site: PyNPyPyPuAPy
*The A later gets activated

Front 3

Does splicing require ATP?

Back 3

YES SPLICING REQUIRES ATP

Front 4

Euk vs. Prok Transcription

Back 4

1 - only 1 prok RNA Pol

2- Prok RNA pol sigma subunit binds seq specifically to Pribnow box at -10

3- Euk need GTF

4- Prok mRNA not post trans modified

5- Pro mrnas polycistronic

5-transc and translation coupled in prok

6- ribosomes bind sequence specifically to mrnas - SD sequence

Front 5

Actinomycin D

Back 5

Transcription Inhibitor:

-intercalates into DNA - prevents elongation

-inhibits RNA transc in EUK AND PROK

Front 6

Rifampicin

Back 6

TRANSCRIPTION INHIBITOR

intereacts in Beta subunit of Prok RNA Pol

cant make first internucleotide linkage

so affects prok only

Front 7

alpha-amanitin

Back 7

TRANSCRIPTION INHIBITOR

low conc = inhibit euk RNA Pol II

high conc = inhibits RNA Pol III

NO EFFECT ON RNA POL I