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7 Cards in this Set

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What are the steps in splicing
1 - U1 recognizes and binds to 5' splice donor site
2-U2 binds branch site and activates A
3-U4,5,6 bind and spliceosome complete
4-phosphodiester bond b/w exon and donor G cleaved by activated A
5-5' phosphate of G forms 5'-->2' linkage with 2'OH of branch site A--> lariate
6-phosphodiester bond b/w acceptor G and next exon cleaved, lariat released, exons joined

NEEDS ATP
What does the intron/exon unit look like?
5' splice donor site - GU
3' splice acceptor site - AG
Branch site: PyNPyPyPuAPy
*The A later gets activated
Does splicing require ATP?
YES SPLICING REQUIRES ATP
Euk vs. Prok Transcription
1 - only 1 prok RNA Pol

2- Prok RNA pol sigma subunit binds seq specifically to Pribnow box at -10

3- Euk need GTF

4- Prok mRNA not post trans modified

5- Pro mrnas polycistronic

5-transc and translation coupled in prok

6- ribosomes bind sequence specifically to mrnas - SD sequence
Actinomycin D
Transcription Inhibitor:

-intercalates into DNA - prevents elongation

-inhibits RNA transc in EUK AND PROK
Rifampicin
TRANSCRIPTION INHIBITOR

intereacts in Beta subunit of Prok RNA Pol

cant make first internucleotide linkage

so affects prok only
alpha-amanitin
TRANSCRIPTION INHIBITOR

low conc = inhibit euk RNA Pol II

high conc = inhibits RNA Pol III

NO EFFECT ON RNA POL I